...selective small molecule FKBP12F36V degraders (dTAG molecule) and expression of FKBP12F36V-tagged proteins. The... C-terminally FKBP12F36V-tagged fusion chimeras or locus-specific knock-in of FKBP12F36V. Detailed protocols...induces ternary complex formation between the FKBP12F36V-fusion and the ubiquitin proteasome machinery...conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867) via various chemical... determine which terminus can accommodate the FKBP12F36V tag without disrupting protein function. A literature...cells expressing a control -- for example, an FKBP12F36V-tagged LACZ) with dTAG molecules in your biological...the doses used to evaluate degradation of the FKBP12F36V-fusion chimera of interest. In the cell lines...

...power of a novel degrader of the 12 kDa protein FKBP12F36V. By fusing FKPB12F36V to your protein of interest...Fig 1. dTag molecules induce dimerization of an FKBP12F36V fused target protein-of-interest and the CRBN...