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Showing: 1 - 20 of 415 results

  1. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...re using a PO number. Follow these steps to place an order online while using a PO number: Log in to your...invoice will reference your Addgene order number and PO number that you entered during checkout. At this...does a customer place an Addgene order using a PO number? Why do I have to order directly from your website... website, and you will be able to enter your PO number as payment during checkout. Placing your order ...addresses provided. How do I place an order using a PO number? Placing an order on Addgene’s website is quite...option to “Pay by Purchase Order.” Enter the PO number you generated through your organization in this...Center article How do I pay by purchase order (PO) number? You can find answers to other ordering questions...

  2. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...normal' sample with known copy number set as the expression baseline. Copy number is then determined by comparing...calculations so that your assay output number (RQ value) matches the copy number of your sample. In that case, ...comparing the number of positive droplets for the gene of interest and reference gene, copy number can be determined...run, as the method is dependent on number of samples rather than number of cycles.  Choosing a method  As...exact copy number and it may not be able to identify trends in cases where the copy number variation is... to quantify said copies. They do so using copy number variation (CNV) assays. There are several varieties...Quantitative/real-time PCR (qPCR) has been used for copy number assays for years. qPCR works like regular PCR, ...

  3. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...plasmid copy number in a cell. One can attempt to eliminate fluctuations due to copy number by introducing...plasmid copy number is the input and gene expression is the output. The input, plasmid copy number, directly...expression due to copy number is kept in check. In such an iFFL, as plasmid copy number increases, the amount...expression and plasmid number. In a suite of plasmids modified from pSC101 with copy numbers ranging from 3-100...growth stage can both affect gene copy number. Moreover, gene copy number is affected differently by growth... plasmid copy number. During the shift from exponential to stationary phase, the number of plasmids per...specific purposes. Unfortunately, plasmid copy numbers vary within cell populations and over time resulting...

  4. Antibody Guide

    Type
    Collection
    ...valency, or number of binding sites available per antibody unit, which is typically the number of antibodies...while the number of proteins that you can detect in a single assay is limited by the number of signaling...therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used in the lab. Check for and...antibody, which becomes the limiting factor for the number of proteins you can detect in a single assay. Overview...

  5. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...Michael J. Fox Foundation Plasmids MJFF has created a number of plasmids for expression of variants of LRRK2...Labs affiliated with MJFF have also generated a number of Shared Research Tools (Link opens in a new window...

  6. CRISPR References and Information

    Type
    Collection
    ...for designing optimal gRNAs. Provides feedback on number of potential off-targets, target's genomic location...in an input sequence, which can be an accession number, genomic location, pasted nucleotide sequence, ...

  7. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...therefore, may not be suitable for use when the number of cells is limited (e.g. primary cells). Most ... to knockout, activate or repress target genes. Number of gRNAs - A given CRISPR library may target the...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...responsible for the bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic ...within the luciferase gene allow for increased copy number in E. coli and expression of luciferase in the ...

  9. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...Looking For? At this time, Addgene has made a limited number of plasmids available as cloning grade DNA that...

  11. TALEN Engineering

    Type
    Collection
    ...REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to additional...

  13. TALEN Guide

    Type
    Collection
    ...Figure 1A ). Scientists have identified a large number of zinc fingers that recognize various nucleotide...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...Kit. These modified pFUS vectors can reduce the number of module plasmids and improve the success rate...

  15. Lentivirus Plasmids

    Type
    Collection
    ...lentiviral transfer plasmid are size (i.e., a limited number of basepairs of nucleic acid can fit into a viral...

  17. Rett Syndrome

    Type
    Collection
    ...further downstream in the NID and C-terminus. A large number of mutations are known to cause Rett syndrome, ...

  19. Plan Your Experiment

    Type
    Collection
    ... target DNA may result in reduced cleavage. The number of alleles for each gene may vary depending on ...

  20. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Plasmids In addition to EYFP and ChR2-EYFP, a large number of additional, validated molecular payloads in ...
Showing: 1 - 20 of 415 results