Performing In Vivo CRISPR Screens Using the FITS Approach
Type
Blog Post
Published
May 2, 2024, 1:15 p.m.
...includes pLX_305_Ovalbumin, a plasmid that enables introduction of the model antigen, Ovalbumin, into cell... of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables efficient knockout ...model in vivo. In this screen, we used the pRDA_526 vector to knock out 30 genes putatively involved in CD8...robustness of the system and utility of the pRDA_526 vector. Moreover, the screen identified putative novel...