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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 23 results for: Psp

Showing: 1 - 20 of 23 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...lentiGuide-Puro. pSpCas9n(BB)-2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n(BB)-2A-GFP ...The top 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid... sequence (pSpCas9(BB)). The 2A-GFP allows for screening of transfected cells. Find pSpCas9(BB)-2A-GFP...nick, rather than cleave DNA. Find pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(BB)-2A-Puro (PX459) - This mammalian...so it may be best to go with pSpCas9(BB)-2A-Puro (PX459) V2.0. Find pSpCas9(BB)-2A-Puro (PX459). Thank...most requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2...the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from the Zhang...

  2. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...initial tests, Cas13b from Prevotella sep. P5-125 (PspCas13b) yielded 62.9% average knockdown, and they chose...chose this enzyme for further studies. PspCas13b does not require a PFS, and it is sensitive to mismatches...They fused ADAR deaminase domains (ADARDD) to dPspCas13b, but observed low RNA editing. To increase A-...template has a cytosine mismatch at that position. PspCas13b-ADAR2DD(E488Q) displayed robust editing with various...showed that editing is mediated by ADAR2DD, not PspCas13b. Find the plasmids for the RESCUE RNA editors ...

  3. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...further ado, the most request plasmid in 2016 was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last... information). V2.0 This designation separates pSpCas9(BB)-2A-Puro (PX459) V2.0 from it's predecessor,...This point mutation has been corrected in V2.0. pSpCas9(BB)-2A-Puro (PX459) V2.0: What's not in the name...

  4. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...postsynaptic potentials (mEPSPs) (Hao and Lee et al., 2024). While studying mEPSPs has been possible with...difficult with GEVIs. Not only was ASAP5 able to detect mEPSPs, it also enabled imaging of their propagation along...

  5. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ... and PE5 prime editing strategies) based on a DeepSpCas9 score ranking (Kim et al., 2019). PRIDICT saves..., H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based model with high generalization...

  9. Viral Production at Addgene

    Type
    Blog Post
    ...produced in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono...

  11. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... HEK293T cells with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260). HeLa cells were infected with...

  12. Immunology Research Plasmids and Resources

    Type
    Collection
    ...islet-derived 1 alpha ICRF, MGC12447, P19, PSP, PSPS, PSPS1, PTP, REG RETN resistin ADSF, FIZZ3, MGC126603...PROK2 prokineticin 2 BV8, KAL4, MIT1, PK2 PSPN persephin PSP PTAFR platelet-activating factor receptor...

  13. CRISPR References and Information

    Type
    Collection
    ...2024) (Link opens in a new window) . gRNA Design DeepSpCas9 (Link opens in a new window) A webtool that uses... for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) Protocol at Addgene Parrott NEBuilder...cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang ...and lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are...

  14. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...meningitidis Gersbach pSPneogRNAH 63556 Other/Leishmania none S. pyogenes Matlashewski pSPneoHHgRNAH 63557 Other...MLM3636 43860 Mammalian BsmBI none S. pyogenes Joung pSPgRNA 47108 Mammalian BbsI none S. pyogenes Gersbach ...backbone 61424 Mammalian BbsI none S. pyogenes Zhang pSpCas9n(BB)-2A-Puro (PX462) V2.0 62987 Mammalian BbsI ...BbsI yes, nick S. pyogenes Puro Zhang pSpCas9(BB)-2A-Puro (PX459) V2.0 62988 Mammalian BbsI yes, cut S. pyogenes...

  15. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral particles...Recommended Materials Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells... transfection: 1 μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid...

  16. Lentivirus Plasmids

    Type
    Collection
    ...with additional packaging components Trono 63586 psPAX2-D64V 2nd Packaging For making Integrase deficient...plasmids along with a plasmid encoding Rev Trono 12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; ...

  17. Lentivirus Production

    Type
    Protocol
    ...Reagent Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol...

  18. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...guanosine, the result is an A->G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence...

  20. Viral Production

    Type
    Collection
    ...produced in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono...
Showing: 1 - 20 of 23 results