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We narrowed to 14 results for: TagRFP

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Showing: 1 - 14 of 14 results

  1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...#49004) to the spectra of both mCherry and TagRFP.  Here, the TagRFP spectrum is shown in the darker colors...peak of the TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission ... emission. For this filter set, we would expect TagRFP to give a brighter signal than mCherry. In general...shorter wavelength red fluorescent proteins like TagRFP or mRuby2 than longer wavelength proteins like ... or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent protein such as ...

  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ... containing several types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags,...NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP, and T2A-TurboGFP-PEST), and small epitope tags...

  5. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...EEA1 EGFP Silvia Corvera 42635 TagRFP-T-EEA1 Endosomes (early) EEA1 TagRFP-T Silvia Corvera 38770 pEF.myc.ER-E2...pTag-RFP-C-h-Rab5a-c-Myc Endosomes (early) Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Endosomes...pTag-RFP-C-h-Rab11a-c-Myc Endosomes (recycling) Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-c-Myc Endosomes...pTag-RFP-C-h-Rab4a-c-Myc Endosomes (recycling) Rab4a TagRFP James Johnson 79799 pTag-BFP-C-h-Rab4a-c-Myc Endosomes...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...can fold in <10min at 37°C, mCherry takes ~15min, TagRFP ~100min and DsRed ~10hours. Temperature: FPs maturation...is set as 1. Some proteins are very dim (e.g. TagRFP657, which has a brightness of 0.1) and this should...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...ipUSEPR-TR657 ATM TagRFP CMV Ataxia telangiectasia Sika Zheng 228918 sg_Atm_i5-ipUSEPR-TR657 ATM TagRFP CMV Ataxia...Huntington's Paul Herman 226380 pcDNA3.1(-).TagRFP.T-2A-Tau2N4R-WPRE MAPT TagRFP, T2A CMV, T7 Parkinson's, FTD Bradley...Bradley Hyman 226381 pcDNA3.1(-).TagRFP.T-2A-V5-Tau2N4R-WPRE MAPT TagRFP, T2A, V5 CMV, T7 Parkinson's, FTD...Bradley Hyman 226382 pcDNA3.1(-).TagRFP.T-2A-V5-8D2Q.Tau2N4R-WPRE MAPT TagRFP, T2A, V5 CMV, T7 Parkinson's...Bradley Hyman 226383 pcDNA3.1(-).TagRFP.T-2A-V5-20D3Q.Tau2N4R-WPRE MAPT TagRFP, T2A, V5 CMV, T7 Parkinson's...Bradley Hyman 226384 pcDNA3.1(-).TagRFP.T-2A-V5-8A2R.Tau2N4R-WPRE MAPT TagRFP, T2A, V5 CMV, T7 Parkinson's...Bradley Hyman 226385 pcDNA3.1(-).TagRFP.T-2A-V5-20A3R.Tau2N4R-WPRE MAPT TagRFP, T2A, V5 CMV, T7 Parkinson's...

  12. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...AAVS1-mTagRFPT-CAAX AICSDP-42 mTagRFPT CAAX domain of K-Ras Plasma Membrane 114403 LMNB1-mTagRFP-T AICSDP...AICSDP-35 mEGFP NA Cytoplasm 101781 CETN2-mTagRFP-T AICSDP-22 mTagRFP-T Centrin-2 Centrioles 101782 LAMP1-mEGFP...protein PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T AICSDP-28 mTagRFP-T Alpha-tubulin Microtubules 101786 ST6GAL1...Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP-69 mTagRFP-T Nucleophosmin Nucleolus (granular component...AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36 mEGFP NA Cytoplasm 114405...

  13. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...dimerization prsTagRFP-N1 - Mammalian Expression prsTagRFP-C1 - Mammalian Expression pBAD/HisD-rsTagRFP - Bacterial... - Mammalian Expression TagRFP675 598 675 4 5 Prone to dimerization pTagRFP675-N1 - Mammalian Expression...Bacterial Expression TagRFP657 611 657 0.34 5.3 27 min Prone to dimerization pTagRFP657-N1 - Mammalian Expression...Expression pTagRFP657-C1 - Mammalian Expression pFA6a-link-yoTagRFP657-Kan - Yeast Expression smURFP 642...Yeast Expression PATagRFP 562 595 405 25 5.3 75 min Prone to dimerization pBAD/HisD-PATagRFP - Bacterial Expression...Expression pBAD/HisD-TagRFP675 - Bacterial Expression mNeptune 600 650 13 5.4 35 min Prone to dimerization mNeptune-pBAD...Mammalian Expression mGeos-M-C1 - Mammalian Expression rsTagRFP 567 585 440 (Off to On) 567 (On to Off) 4.0 6.6...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...emission spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors...
Showing: 1 - 14 of 14 results