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We narrowed to 8 results for: bifc

Showing: 1 - 8 of 8 results

  1. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ... a single BiFC pair. Find BiFC plasmids for your experiment at Addgene! How to Design a BiFC Experiment...supraphysiological expression levels. BiFC quantification This is where BiFC outshines FRET — BiFC experiments only require...Much like FRET, BiFC experiments must be carefully optimized to be successful. A BiFC experiment generally...mutants to identify those that inhibit BiFC in your cellular system. BiFC competition analysis can be used ... Kerppola (2006, 2013). BiFC vs. FRET: When to choose one over the other BiFC is a convenient, fluorescence-based...PPIs): Bimolecular Fluorescence Complementation (BiFC). What is bimolecular fluorescence complementation...potential protein interaction partners. Unlike FRET, BiFC only uses a single fluorescent protein to visualize...

  2. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...for FRET and ratiometric BiFC from Christopher Grefen’s lab or explore the MoBiFC modular bimolecular fluorescence...known as Bimolecular Fluorescence Complementation (BiFC). It can provide a qualitative or quantitative readout.... Biotechniques. 2010 Chang-Deng Hu mVenus pET-BiFC contains both fragments of split mVenus (aa 155)...formation (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein ...exceptions to the usual split-FP options. While the BiFC interaction is typically irreversible and requires... You can read our previous blog post for more on BiFC principles and experimental design. We hope you ...B. PLoS One. 2020 Bo Huang Yellow Venus pBiFC-VN173, pBiFC-VC155 Identification of new fluorescent protein...

  3. Bacterial Expression Systems

    Type
    Collection
    ...acceptor) FRET Wolf Frommer 87856 pET-BiFC mVenus (reconstructed) BiFC Dan Mulvihill 39866 pWA PAS-E BAD ...reconstructed) BiFC Lynne Regan 168257 168472 pMRBad-C-wtBlc pET11a-N-DiB2 DiB2 (reconstructed) BiFC Jens Meiler...FRET) and bimolecular fluorescence complementation (BiFC) are techniques that have been widely used to study...acceptor fluorophore by only exciting the donor. In BiFC, proteins of interest are fused to each part of ... your proteins of interest in bacterial FRET and BiFC studies. ID Plasmid Fluorescent Protein Application...BAD K-GAFm iRFP (reconstructed) BiFC Vladislav Verkhusha 52732 52733 pET11a-link-NGFP pMRBAD-link-CGFP GFP...FRET biosensors for ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222) is a collection of...

  6. Zebrafish Plasmid Collection

    Type
    Collection
    ... vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate model to...
Showing: 1 - 8 of 8 results