... spectra of SF-Venus-iGluSnFR.A184V, iGluSnFR3 v82, and iGluSnFR3 v857. Image adapted from Aggarwal et...summarized quickly as: iGluSnFR3 v82: increased affinity and slower kinetics iGluSnFR3 v857: decreased affinity...imaging or in new preps. Using iGluSnFR3 in your lab The new iGluSnFR3 variants should enable scientists...SF-iGluSnFR), and yellow (SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging...iGluSnFR), Kaspar Podgorski’s lab have recently made iGluSnFR3 variants that are more sensitive and better localized...the new variants is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, the researchers...photophysical properties The improved variants of iGluSnFR3 have higher expression levels and produce stronger...

...reporter (iGluSnFR3) to study glutamate, a signaling molecule in the brain. “We hope that iGluSnFR3 will enable...and Tim Hanson, are characterizing variants of iGluSnFR3 in vivo and have used it to detect glutamate release...these biosensors.   Figure 1: Schematic of iGluSnFR3 (left). GluSnFR3 in pMinDisplay (PDGFR anchoring...

.../s41592-022-01597-x. New glutamate indicator iGluSnFR3 Improving on the glutamate sensor iGluSnFR from...Kaspar Podgorski and colleagues have now released iGluSnFR3 (Aggarwal et al. 2022). This new version has higher...from the authors in this blog post.   Figure 2: iGluSnFR3 displays enhanced brightness, expression, and ...SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3 plasmids here: https://www.addgene.org/browse/...In addition to the viral preps noted above for iGluSnFR3 and JEDI-2P, several biosensors already in our...article/28220233/ Find viral prep here: pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt https://www.addgene.org...

...sharing the data on the mAb3D atlas website.  The iGluSnFR3 Glutamate Sensor: Guest blogger Abhi Aggarwal ...

...Constitutive 5 Khakh 175180 pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt Syn iGluSnFr3 v857 none Cre dependent...dependent 1 Podgorski 175181 pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt Syn iGluSnFr3 v857 none Cre dependent...dependent 1 Podgorski 178329 pAAV.hSyn.iGluSnFR3.v857.PDGFR Syn iGluSnFr3 v857 none Constitutive 1 Podgorski...Podgorski 178331 pAAV.hSyn.iGluSnFR3.v857.GPI Syn iGluSnFr3 v857 none Constitutive 1 Podgorski Glutamate Sensor...

...Glutamate Third generation glutamate sensors (iGluSnFR3) for improved imaging of synaptic transmission...