...stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems...fluorescent CRISPR reporter methods, SRIRACCHA and GEMCherry2. We will cover single base-pair editing CRISPR...genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein for...monomeric red fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate Cas9...and activity (Knudsen et al., 2018).  To create GEmCherry2, the Denham lab first incorporated an out-of-...the methionines at position 14 and 21 to create GEmCherry2. The Denham lab made several other small changes...creating 2 other versions but these did not surpass GEmCherry2 in background fluorescence and in-frame fluorescence...

...Yeast Expression mCherry2 587 610 Monomer mCherry2-N1 - Mammalian Expression mCherry2-C1 - Mammalian Expression...HisB-PAmCherry1 - Bacterial Expression PAmCherry2 570 596 13 6.2 18 min Monomer pPAmCherry2-C1 - Mammalian Expression...

...addition, some versions of the mAID vectors come with mCherry2 or mClover fluorescent proteins allowing you to...