Empty Backbones - Choosing Your Perfect Plasmid Backbone
Type
Collection
...- N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector for Myc tag pETcon(-)...AdEasier®-1 cells
(strain) - Bacterial strain that contains
AdEasy®-1 plasmid...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...expression under a CMV
promoter pAdEasy®-1 - Recombine
plasmids from the shuttle... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 ...lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning and...