Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
Type
Blog Post
Published
April 25, 2017, 2:30 p.m.
...Aequorea victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine (T203H) (1). wtGFP normally contains ...chromophore which contribute to the 2 peaks of wtGFP’s excitation spectrum- a major 397 nm peak and minor...minor 475 nm peak, respectively. When wtGFP is illuminated with UV-violet light (~400 nm), the chromophore...