Plasmids 101: Protein tags
Type
Blog Post
Published
Dec. 11, 2014, 4:26 p.m.
...best optimized is the tobacco etch virus (TEV) protease. A TEV protease cleavage site is frequently placed... 2: Protease recognition sites commonly used with tags
Protease
Recognition site
Notes
TEV
ENLYFQS... of a calmodulin binding peptide (CBP), a TEV cleavage site (more on that in a moment), and 2 ProtA IgG-binding...specific proteases without requiring the addition of an independent cleavage recognition site. In fact...purification, with the cleavage reaction taking place between column runs. The TEV protease itself, with various...antibody-based purification; has inherent enterokinase cleavage site
GST
Large Protein
26
Purification and Stability...efficiency of this cleavage depends on the identity of X.
A number of other proteases are available, but...