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We narrowed to 320 results for: POR C

Showing: 1 - 10 of 320 results

  1. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...response gene c-fos. For example, using Chr2 stimulation should result in robust increases in c-fos expression...after stimulation compared to controls without Chr2. c-Fos is a reliable and widely used molecular marker...proceed with your behavioral experiments. Comparison of c-fos amongst animals that receive different types of...neuronal activation, but it may still be possible to use c-fos expression to get the evidence you need. For example...activation in the novel context as determined by reduced c-fos expression, thus showing successful inhibition... as non-human primates [7, 8], zebrafish [9], and c. elegans [10]. If used properly, optogenetics is sure...but, in today’s post, we’ll discuss some of the important considerations you should think about when developing...

  2. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...future rare disease research funding opportunities. References 1. Jones, C., et al. "PP09. 1–2352: Systematic...disease are unclear. Dr. Di Schiavi plans on using C. elegans as a model to identify SMN1 interacting partners...effects of smn1 disruption. As part of his work in C. elegans, Dr. Di Schiavi will use Addgene plasmids...these genes, CRISPR to disrupt their function, and C. elegans overexpression plasmids to upregulate their... from rare diseases by connecting patients to important resources and promoting research for rare disease...Cancer Alliance Website.  We are proud to provide support to Drs Di Schiavi and Nabavi and will continue ...Blog Previous BeHeard Awardees Other Efforts to Support Rare Disease Research: IFOPA Donating Plasmids ...

  3. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...Y (T or C), X (any base), or C, respectively. gGBE, gTBE, gCBE Glycosylase-based G, T, or C base editors...transversion editors: CGBEs, or C → G Base Editors. CGBEs first convert a cytosine (C) to uracil (U), just like... a more specific A → C base editor (ACBE), they inserted the deaminase into the C-terminal domain of Cas9...pyrimidines (C ↔ T).   Base transversion Base change from purine (A or G) to pyrimidine (C or T) or vice-versa...type of base editor invented. CGBE C → G Base Editor Converts C → G by fusing a uracil DNA glycosylase..., or C, respectively, to be replaced with another base. References Chen, L., Hong, M., Luan, C., Gao... Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa, A. A., Qu, C., Zhu, Y., Guan, Y., Wang,...

  4. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...transversions C → TG → A A → GT → C C → G                   C → AG → C                   G → T A → C                   ...Rosser, S. J., Bi, C., & Zhang, X. (2021). Glycosylase base editors enable C-to-A and C-to-G base changes...deaminase could also perform cytidine (C) deamination, converting the C base to uracil (U). This is the same...given the pithier name CGBE1, for “C → G Base Editor 1,” and had a C → G editing efficiency of up to 68%...Beyond UNG: Optimizing C → G editors Now that several labs had shown that preferential C → G editing was possible...pyrimidines (C ↔ T).   Base transversion  Base changes from purine (A or G) to pyrimidine (C or T) or vice-versa...type of base editor invented. CGBE C → G Base Editor Converts C → G; also called a glycosylase base ...

  5. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ... 22nd International C. elegans meeting, I was again reminded of the extent that C. elegans researchers...and toolkits available at Addgene.  C. elegans open resources The C. elegans Neuronal Gene Expression Map...database specifically for C. elegans. This database provides detailed information about C. elegans 3’UTRs structures...UTRome The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans. To study the role...The C. elegans community has always emphasized the need for open science and collaboration. The field...view the anatomy of worm layer by layer, the NeuroML C. elegans connectome, a model of the C.elegans neural...be used to simulate muscle tissue as it applies to C. elegans locomotion. To see the other resources included...

  6. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...for use in C. elegans. In 2017, the Korswagen lab developed Cas12a (Cpf1) for editing in C. elegans (Ebbing...editing from more sites of the C. elegans genome. Toolkits for editing in C. elegans The development of ...SapTrap-SEC kit for C. elegans genome engineering have also facilitated CRISPR editing in C. elegans. These...2018). To see more CRISPR tools for C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future...Ruvkun for their seminal work on micro RNAs in C. elegans. The C. elegans work on small RNAs informed and drove... countless other systems, whereas import of CRISPR technology into C. elegans has been a transformative...possible in a wide range of organisms and cell lines. In C. elegans, one of the model organisms which I use to...

  7. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...bound to the C-terminal peptide. One of these C-terminal antibodies was Myc1-9E10 (aka anti-c-Myc [9E10]...sequences within the middle of the human c-Myc protein or the C-terminus, Evan, et al. isolated six different...that Myc1-9E10 and the other C-terminal antibodies only recognized the human c-Myc protein and did not recognize...experiments? Why Myc? When did the commonly used anti-c-Myc [9E10] antibody come into play? We’ll dive into...This protein had a highly specific antibody to the C-terminal five amino acids, meaning the tag could be...However, substance P has an amide group added to its C-terminus post-translationally, which turned out to.... As such, the peptide could only be added to the C-terminus of proteins of interest and proteins tagged...

  8. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...genome engineering methods for C. elegans were released, signaling a new era in C. elegans research. Homology...of custom genomic modifications, is very robust in C. elegans, and the methods for HDR-mediated modification...the University of North Carolina has made CRISPR in C. elegans even easier - now, one can generate a fluorescent...-based gene tagging approach Multiple features of C. elegans have made CRISPR/Cas9 so successful in this...previously used a transgene selection strategy for their C. elegans/CRISPR experiments, but this strategy required... make it possible fluorescently label proteins in C. elegans, they inserted the SEC into a synthetic intron...system is both simple to use and highly efficient for C. elegans genome modification. Figure 2: Schematic...

  9. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ... and C-C’) that give the feature its characteristic T-shape. The arrangement of the B-B’ and C-C’ palindromic... Tian, W., Liu, C., Lian, Z., Dong, X., & Wu, X. (2017). Deletion of the B-B’ and C-C’ regions of inverted...contains three palindromic sequences (A-A’, B-B’, and C-C’ regions) and forms a T-shaped structure. The Rep... et al. 2017). For example, deleting the B-B' or C-C' arms can reduce the packaging efficiency but increase...most common variant is an 11 bp deletion in the B or C domain of one ITR. These small deletions are not usually... Conatser, L. M., Lue, V. M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated...AAV plasmids contain several important elements, but among the most important are the inverted terminal ...

  10. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...the C-terminal region of the human proto-oncogene c-Myc. Since the generation of the first anti-c-Myc ... on the Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody...potassium channelrhodopsins Myc-tag antibody anti-c-Myc [9E10] now available! Improved voltage indicator...and tdTomato expressed layer 2/3 neurons in mouse. C) Action potential frequencies with (magenta) and without...10.1101/2021.09.17.460684.)   Myc-tag antibody anti-c-Myc [9E10] now available! by: Ashley Waldron The majority...pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a) antibody is currently recommended...Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. Dec;5(12...
Showing: 1 - 10 of 320 results