We narrowed to 58 results for: mcherry

...CRISPR genomic target region into the N-terminus of mCherry. To prevent alternative start site initiation, ...GEmCherry1, due to the “genomic fragment inserted into mCherry”. However this one mutated methionine was not enough...genomic region inserted into the N-terminus of mCherry can be targeted by a sgRNA guided Cas9 causing ...or deleted, can result in an in-frame shift of mCherry and thus expression. As a proof of concept the ...this experiment they saw a clear difference in mCherry expression using FACs sorting, confirming yet again...

...pAAV-Ef1a-fDIO mCherry Karl Deisseroth 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP Brandon...pAAV-hSyn-DIO-mCherry Bryan Roth 50457 AAV1 pAAV-hSyn-DIO-EGFP Bryan Roth 114472 AAVrg pAAV-hSyn-mCherry Karl...pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry Connie Cepko 83898 AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 Gordon Fishell...Flp-dependent expression of ChR2(H134R) bound to an mCherry reporter. We now also offer AAVrg for plasmid 83898... Depositor 44361 AAV1 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Bryan Roth   Recombinases Recombinase systems...Serotype Name 44362 AAV1 pAAV-hSyn-DIO-hM4D(Gi)-mCherry 83896 AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell...Plasmid Serotype Name 114471 AAV2 pAAV-Ef1a-fDIO mCherry 83900 AAVrg pAAV-mDlx-GFP-Fishell-1 Biosensors...

...anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in ...gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely...these viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor...receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control of the human synapsin promoter. Purpose... Purpose of this AAV pAAV-hSyn-DIO-hM4D(Gi)-mCherry can be used to induce neuronal silencing in vitro ...Aschauer et al., 2013). Expression of hM4D(Gi)-mCherry from this virus is cre-dependent, meaning that ...2011). Viruses expressing pAAV-hSyn-DIO-hM4D(Gi)-mCherry are typically injected into brain regions of mice...

...pAAV-CaMKIIa-hChR2(H134R)-mCherry 26976 AAV5 pAAV-hSyn-hChR2(H134R)-mCherry 26973 AAVrg pAAV-hSyn-hChR2...pAAV-hSyn-DIO-hM3D(Gq)-mCherry    44362  AAV1 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Controls Plasmid ...pAAV-hSyn-DIO-mCherry 27056 AAVrg pAAV-Ef1a-DIO EYFP 114471 AAV5 pAAV-Ef1a-fDIO mCherry 112677 AAV2...pCAG-FLEX-EGFP-WPRE 114471 AAV1, AAV5 pAAV-Ef1a-fDIO mCherry 99130 AAVrg pAAV-mDlx-NLS-mRuby2 112677 AAV1...AAV1, AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 27056 AAVrg pAAV-Ef1a-DIO EYFP 50457 AAV2 pAAV-hSyn-DIO-EGFP...AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 50457 AAV2 pAAV-hSyn-DIO-EGFP Recombinases Plasmid...

... with mCherry and you already have a transgenic mouse line that contains a Cre-dependent mCherry cassette...with mCherry controlled by a neuron specific promoter or you could breed your Cre-dependent mCherry line...incredible growth this year. pAAV-hSyn-DIO-hM4D(Gi)-mCherry was the top requested AAV prep for the 2nd year...you need would be to inject your Cre-dependent mCherry mouse with the pmSyn-EBFP-Cre AAV. Because Cre ...controlled by the synapsin promoter, it and therefore mCherry will only be expressed in neurons. Indeed, this...

...these experiments.  AsLOV2 Inertion Site mCherry #1 mCherry #2 GFP Loop 1 dark-induced dark-induced...Toettcher Lab monitored how AsLOV2 insertion into mCherry or GFP nanobodies affected binding of their target... its co-localization with a membrane-localized mCherry or GFP protein. With this system, if the OptoNB...sites in all eight surface-exposed loops of an mCherry nanobody. Only insertion into loops 1 and 6 resulted...scheme to generate two more OptoNBs, one against mCherry and one against GFP. Insertion of AsLOV2 into loop...which revealed a blue light-dependent shift in mCherry fluorescent to the surface of the bead, suggesting... a membrane localized GTPase, the team fused a mCherry OptoNB to the catalytic domain of the Sons of Sevenless...

...pAAV-FLEX-tdTomato pAAV-GFAP104-mCherry pAAV-mDlx-NLS-mRuby2 CAG-NLS-GFP pAAV-hSyn-mCherry Read on to learn more...pAAV-FLEX-tdTomato, pAAV-GFAP104-mCherry, pAAV-mDlx-NLS-mRuby2, CAG-NLS-GFP,and pAAV-hSyn-mCherry at Addgene. Viral ...114472  AAV5, AAV8  pAAV-hSyn-mCherry 50459  AAVrg  pAAV-hSyn-DIO-mCherry 59462  AAV2  pAAV-CAG-tdTomato...Serotype Name 75033  AAV1  pAAV CD68-hM4D(Gi)-mCherry (CD68 is a  microglia/macrophage promoter) 121539...pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1  pAAV-EF1a-Flpo Calcium...

...interest. The green fluorescent protein (GFP) and mCherry, two of the most common tags currently used, are...used a construct in which the hM4Di was fused to mCherry. To investigate the localization of the hM4Di, ..., we used primary antibodies against mCherry, and secondary antibodies coupled to nanogold particles, ...proteins Given the limited expression of hM4Di‐mCherry at the plasma membrane in monkey neurons, we next...small change made a big difference. Replacing the mCherry with the HA tag resulted in abundant expression... of the transgene of interest. In our studies, mCherry interfered with the transport to the membrane of...technique was used to reveal the hM4Di coupled to mCherry (top) or HA tag (bottom). The images show examples...

... mutated mCherry and a downstream constitutively active eGFP. To create the inactive mCherry, the lab ...downstream of wild-type mCherry and a T2A site and expressed in cells. mCherry is constitutively expressed...dividing the number of eGFP and mCherry positive cells by the number of only mCherry positive cells (Martin et... (5’-TCA-to-TUA) found within the 43 base-pair mCherry insertion. Editing of these motifs generates lesions...two DSBs that can be repaired by NHEJ restoring mCherry expression. Cells can then be sorted using Fluorescence-activated...only eGFP expression to cells expressing eGFP and mCherry. ACE was used to identify cells that have been ... relies on the restoration of a frame-shift in mCherry that restores fluorescence to monitor APOBEC-Cas...

...VVD and the DNA-binding domain of AraC using a mCherry reporter downstream of a PBAD promoter. While none...created two constructs, among others: pBLADE-mCherry: This construct, built from the pBAD33 backbone...backbone, contains BLADE, the PBAD promoter, and a mCherry reporter downstream of the PBAD promoter. To use ... with your gene of interest, you would replace mCherry with your gene of interest. pBLADEONLY: This plasmid...with pBLADE expressing superfolder GFP instead of mCherry under the PBAD promoter. They created a bacterial...