... cost to make a plasmid? Doench also calculated the cost of consumable reagents used to make a plasmid...after ordering, making it a toss-up as to whether it’s faster to order or try to make it in your lab (...works for your experiment. Make your work “high-throughput” Instead of making one plasmid at a time, try...Leeson. Have you ever wondered how long it takes to make a plasmid? Or how much time you have to spend cloning...we wrote a post on the time and cost required to make a plasmid. In 2023 (and at closer to 2 million plasmids...updated this post. How much time does it take to make a plasmid? Figure 2: Survey results from a...Flame depositor John Doench calculated the time to make a plasmid. Doench’s lab in the Broad Institute has...

... the heavy lifting out of CRISPR vector design, making it easier to get the vectors that are just right...six or seven components — in most cases, that's making two or more plasmids — it may be more efficient...Fragmid doesn’t have the components I want? You can make your own Fragmid components! Ensure your backbone...

...should be on their website or the program booklet. Make sure you’re familiar with the policies before posting... tips not covered here, head over to the “How to Make Friends and Meet People at a Scientific Conference...

...needed to perform GCE are essentially those needed to make site-directed mutagenesis, many researchers new ...for the use of GCE. In short, they’re working to make GCE available for all biomedical researchers. If...

... events will generate these types of mutations, making it an ‘easy’ editing job to get done. However, ...repair and genome engineering The easiest way to make a knock-in cell line is to utilize the built-in ...observed when inserts are within 10 bps of the break, making the cut off essentially as part of the templated... distance. Homology arms and types of donors To make sure your donor molecule is used as the template...knock-in site. The length of that sequence and the makeup (single vs. double stranded) all depends on the...If your Cas9 cuts in the coding region of a gene, make sure that the PAM edit you introduce is a silent... and after Cas9 introduction increases HDR, just make sure to remove the inhibitors within a day or two...

... when making decisions, assignments and polices. Sometimes the boss has to be the boss and make difficult...obstacles.” – Scott Adams, Dilbert cartoonist What makes a good manager? If that is all it takes, then how... aside from a focus on removing obstacles, what makes a good manager? First, it takes an open mind to ... from being included in organizational decision-making. It is imperative that employees are thanked for...a good performer if they are enough of a jerk to make the rest of the team miserable.   A good manager...

...a good idea. Make contact with other attendees in advance. Join a LinkedIn group (or make one!) or find...is essentially no better place for a scientist to make new relationships than at scientific conferences...you see enough of them).  Before the conference Make sure you have a business card – even students and...will attend. Research the speakers in advance and make a note of those you would like to try and meet. ...members that have moved on to see if they will attend. Make a date or two for beer, coffee or meals with colleagues...memorable meal is a great way to build relationships. Make a list of experimental problems or questions you...poster roll, say hello. Keep your cards handy and make sure your name tag is always visible. Some people...

...use in your own lab. How to make your own competent cells It’s easy to make your own competent cells. If...need to be cost and time efficient. We do this by making our own competent cells and using a little-known...will work just fine.  A number of protocols for making competent cells are available online. A few examples...Transformation Kit and Buffer Set  from Zymo Research to make competent cells because cells prepared using this...instructions that came with them. If you decided to make your own competent cells using the protocols provided...control (plate your cells without adding plasmid) to make sure you don’t have contamination.  Also conduct...

... I found Vectr to be fairly intuitive and I was making figures quickly. Inkscape Inkscape is the most...🔬! We built this so you could stop struggling to make figures in PPT 🔬 Also, the base version is free...

...scientists ask me about mentoring. Here they are: What makes a good Mentor? How do I choose an Advisor/PI who... benefits of taking on my own Mentee? Q1. What makes a good science mentor? I started a conversation ...their experience. This led to the topic of “What makes a good Mentor?” Some of the Tweeted comments: “...good Jedi does not necessarily an excellent mentor make. Are your current mentors/advisors like Yoda? If...for you) Helps identify a Mentee’s strengths and make the most of them Is not threatened by the Mentee...

...individual features that make each tool unique. The CRISPR Software Matchmaker is composed of these features... the end of 2014, I began developing software to make sgRNA design accessible to all. At the time I thought...over another. On the other hand some software tools make use of empirically derived and/or prior knowledge... and parameters. This tapestry of ideas is what makes choosing the right tool confusing and it means that...at the glossary section of the CRISPR Software Matchmaker, it provides a list of terms I think are important...going shopping for one. Use the CRISPR Software Matchmaker to select the best tool based on your needs. ... and cherry pick the features that will actually make a difference in our experiments and lives. To do...

...studied PIPs. The straightforward nature of BiFC also makes it easier to generate a large number of constructs...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection. Cellular expression Whether...localization may differ from those of your BiFC construct, making it difficult to interpret your results. Unfortunately...cells. The ease with which BiFC can be visualized makes it amenable to studying PPIs under relatively physiological...

...the Victoria Makerspace in Canada, which I belong to. The team at the Victoria Makerspace is composed ...known mutants at $7.00 a strain. Makerspaces like ours can also make accounts with  Addgene. We now have...of the aspects of this project that makes it ideal for makerspaces is the data is freely available, and...  Funding Makerspaces One of the main challenges when starting a new project at a makerspace is finding...following post was contributed by Derek Jacoby from Makerspace Victoria, CA Over the decades science has become...manifested itself in the creation of Hackerspaces and Makerspaces back in 2007, which function as centres for peer...lectures. There are currently around 1,000 active makerspaces around the world. Hackerspaces.org maintains ...

...experience and make sure your next team works better. In fact, don’t wait to be invited onto a team. Make one yourself...Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d spent my senior year ... For example, when it came time to update how we make and distribute kits, I helped assemble a crack team...Is the goal of your project to launch a product? Make an informed decision about something? Fix a problem...indeed. Mission team A Mission Team is assembled to make progress towards open-ended goals whose metrics ...Team, a like-minded group of co-workers who want to make our organization as environmentally friendly as ...There’s also no end-game here, unless we manage to make Addgene the most environmentally friendly organization...

...they don’t make in wages, they make up for in training. Unfortunately, these low wages also make it so it... trainees pay may also make them more attuned to the bang for their buck - make them demand more services...answered about the makeup and welfare of the many researchers out there who are making great progress on...academic research community to develop a plan to make postdoctoral pay/benefits commensurate with work...how many post-docs there are in the U.S., their makeup (in terms of nationality, gender, race, etc), or... One of the Saturday Hack Day projects sought to make an interactive game that highlights just this point...will help you in your academic publishing goals and make you a better communicator overall. Don’t Write...

...reasoning out the window when making decisions. We think scientists make choices based on logic and reason...’s actually something you CAN choose and it will make a huge difference for your future career and life...Which route should I take to get to work?”; we often make important life decisions without much logic - even...your own style of work and communication before making this important decision. I’ll highlight some of...matter to me?”  and hone in on the factors that will make the most difference for your success. Gather data...of written questions for the PI and lab members.  Make coffee or beer dates to talk to as many lab alumni...energy level in the lab? Does a frenetic atmosphere make you edgy or energized?   Are you an only child and...

...online). Mentee: Make a list of topics/skills you are interested in exploring. Mentor:  Make a list of topics...your career development as your mentor. How do you make the most of this new relationship? Consider adding...take this past the chatting stage you will rarely make any change in your skill sets or activate the helpful...that need work or growth. Here are some ideas for making your meetings productive: Discuss logistics if...communicate between meetings (email, phone, etc.)? Make agendas for your meetings, take informal minutes...goals with a mentor: The secret of accountability To make a mentoring relationship really productive, the ...many mentoring meetings to hone the goals and to make them “SMART.” SMART goals are Specific, Measurable...

...Read this Catalyst Report “Making Mentoring Work” to learn about what makes a good mentoring program. ...guidance. Second, I will offer some advice on how to “make the ask” once you have found someone you admire ...professional presence, approach them for advice on what makes them that way. Is there a senior person whose career...Choose 1-2 tactics to try. One of my favorites is making the pledge to have lunch or coffee with someone...able to: Help identify the skills you will need to make a change Provide perspective on core values at another...mentoring program, consider tapping into it and helping make it work by being an educated mentee. Other opportunities...and time. Consider this option but be cautious and make sure to choose one that comes highly recommended...

...BioBrick parts to make more materials: to make more fuel, to make more chemicals, to make more medicine. ... of DNA, transcription of DNA to make RNA, translation of RNA to make proteins. That's central to everything...systems, what do you want to make? So, tell us readers, what do you want to make?   References: Jerome Bonnet...BioBricks Foundation is seeking to accomplish is to make the engineering of biology much easier. So you could...much fun and so incredibly useful that we wanted to make them free to use by everybody. (For more on these...really plugging into the "stuff sector" if you will, making chemicals and materials, and we anticipate plugging...imagination. The question is: what can you use biology to make? If you think about the BIOFAB collection and really...

...let’s chat about giving science talks and how to make them serve you well. The happy byproduct might just...results in the order they were performed does not make for a memorable presentation anywhere but in a lab...knowing your audience is that it can help you avoid making mistakes. I once made a joke about social science... story. Using stories is one of the best ways to make you and your presentations engaging and memorable...you are more likely to remember the point.   What makes a story? A story is a narrative connecting cause...all the time, you just need to figure out how to make it suspenseful. 4. Start preparing your talk early...them. Of course, if some of your work does relate, make sure to include that material, but don’t force it...

...off your work at a conference, you need to first make your poster. While a poster generally contains the...participants can identify you. You may also wish to make creative use of a footer in your poster. This is...sections, so you can include a lot more. Font size: Making your poster readable While we are talking about...This strategy helps your sections stand out, and makes the entire poster more navigable.  Choose your content...that it will display your data most prominently, making it the star of your presentation. Portrait. This...potentially more catchy, but it can be trickier to make figures fit nicely into circular shapes rather than...color you select for the background, you’ll want to make sure that the color of your font is compatible. ...

...volunteer to lead a charitable cause.  While I can’t make it “all better” for you, I can offer some tips and...that end, here are my personal 10 commandments for making it all work – most of the time. #1 Thou shalt find... so will your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead...management tools and tactics to plan your work and make this happen. Be realistic and: Your co-workers ...done your way. Be patient with yourself; you will make progress over time. #6 Thou shalt throw money at...and deliver your groceries. Do anything you can to make your life easier and to have more time to relax ...enthusiastically foster independence Your partner can make his or her own dinner if you are going away to a...

...doi.org/10.1038/nrmicro3569 Makarova, K. S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S.,...Moya-Beltrán, A., Makarova, K. S., Acuña, L. G., Wolf, Y. I., Covarrubias, P. C., Shmakov, S. A., Silva, ...type III, and type IV. Type I Type I CRISPR systems make up the majority of Class 1, and are the most common..., F. E., Moeller, L., Kocalar, S., Oshiro, R., Makarova, K. S., Macrae, R. K., Koonin, E. V., & Zhang,...doi.org/10.1126/science.adi1910 Chylinski, K., Makarova, K. S., Charpentier, E., & Koonin, E. V. (2014...doi.org/10.1134/s0006297921100114 Koonin, E. V., & Makarova, K. S. (2019). Origins and evolution of CRISPR-Cas.../doi.org/10.1098/rstb.2018.0087 Koonin, E. V., Makarova, K. S., & Zhang, F. (2017). Diversity, classification...

...community: Diversity makes the difference  Association for Psychological Science: Diversity Makes Better Science...formally, governments are the organizations that make and enforce the rules of the lands we live on. Rules...cannot declare that gravity change direction and make things fly up when we drop them. Scientific realities...to an earlier analogy, no amount of shouting will make an apple fall up. As scientist and politician Rush...will fall up instead of down when dropped will not make it happen. In a time when a top official can be ... the job of having evidence”, scientists want to make sure having evidence is the job of everyone in government...Immigration Pollution Healthcare Science education To make scientists relatable (17, 20). This is an effort...

... information makes your manuscript more valuable to the research community. It also makes it more likely...Materials and methods section of their manuscript to make resources easier to identify, find back, and share...enough information to replicate your experiments, making research more reproducible and your results more...reviewers and other researchers to access the data and make up their own minds on whether the data analysis ...reliability of science publications. Peer reviewing To make sure your manuscript is perceived as a reliable ...ts when publishing a scientific manuscript Dos: Make sure your paper fits the scope of the journal you...Guidelines of the journal you are submitting to and make sure your manuscript fits the house style and prerequisites...

...organizations have been working to provide tools that make it easier to share in-depth information about reagents...FPbase are working hard to enable researchers by making it easier to find information about research tools... more descriptive and interactive protocols that make it much easier to learn new experimental techniques...Electronic lab notebooks also play a role here by making it easier to retain searchable, thorough notes ...techniques, an important component of reproducibility is making sure that others can understand your analyses and...data analysis while other organizations like Dryad make it easier for researchers to share data thereby ...published data if you need to. Similarly, FigShare also makes it easier to share data through their curation of...

... to produce constructs and edit genomes so as to make plants, and agricultural crops in particular, resistant...general. We are funded by a charity and since we are making all the tools for plant research here and validating...like for people to use them. The MoClo Plant Kit makes it really easy to clone genes for expression in ... the time. Now there are many, so if you want to make a 10-gene construct, you don’t have to worry about...plants, including many things people already use. It makes the switch to Golden Gate Cloning easier because...pathogens. It’s also easy to express genes in, which makes it a good laboratory workhorse. Tips & tricks Addgene... that you need from the kit and getting started making your own constructs with Golden Gate MoClo. The...