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We narrowed to 148 results for: plasmid repository

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  1. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...through a repository, they remain accessible during these times. Centralized reagent repositories, also known... Figure 1: Biological repositories that distribute mouse strains, plasmids, cell lines, and much more...these plasmids from Addgene. This publication then resulted in the development of a new plasmid, pCMV14...continue having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for COVID-19 research...dedicated facilities and manpower that a repository provides. Repositories around the world facilitate the rapid...to be deposited into repositories, and financial support to maintain repositories’ capacity for storing... the scientific community can support repositories. Repositories are essential for the advancement of ...

  2. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...18,359 viral items, and 166,247 plasmids. We welcomed 18,899 plasmids, deposited from 1,106 labs in 504... countries, into our repository, while expanding global access to our repository through our distributor...vectors. At 3500 plasmids, this represents the largest single deposit in our repository and was a three–year...announce that we are now able to ship rabies plasmids in our repository to international requestors.    I would...14 deposits, that became available through our repository in 2023.  In June, we announced a new partnership...September, we released the fourth edition of our Plasmids 101 eBook. This updated version of one of our ... launch our Antibody Data Hub, an open-access repository for antibody validation data. We’ll continue ...

  3. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ... in one place. Plasmid mapping These tools are for viewing, editing or making plasmid maps, but can also...make plasmid maps. Free for academic users. Serial Cloner: Free desktop-based software for plasmid design...design and mapping. ApE (A plasmid Editor): A free, donation-based plasmid analysis tool including editing...Utah.   Figure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA.   DNA / ...data repository for validation data on Addgene/CLOVER viral vectors.  Caspedia: A data repository for ...Primer design. Plasmid mapping. DNA sequence analysis. We all have our favorite tools for tackling these...The free SnapGene Viewer is great for looking at plasmid maps and viewing sequencing traces, while the paid...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...sharing of plasmids between scientists. The service would allow people to add a gene to a repository others... years to reach our first major repository milestone — 10,000 plasmids deposited and available — and nearly...the 500,000 plasmids distributed milestone! We were thrilled to see the impact our repository was having...20th anniversary and the year our repository grew to over 150,000 plasmids, shipped out our two millionth...frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan, and Benjie...meant we could grow accessibility alongside our repository.  “I’m sure you hear it all the time, but Addgene...Addgene before we were a “labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP...

  5. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...you out. What is the Open Repository of CRISPR Screens? The Open Repository of CRISPR Screens (ORCS) database...CRISPR guide Learn more about plasmid pooled libraries Find CRISPR plasmids for your research ...This post was contributed by the Open Repository of CRISPR Screens. Imagine you’ve just discovered that...research. Fortunately, there’s a growing open repository of CRISPR screen data that might be able to help... resource developed by the Biological General Repository for Interaction Datasets (BioGRID) in order to...biologists. Figure 1: The BioGRID Open Repository of CRISPR Screens (ORCS) homepage. Screenshot...Screenshot updated 2024.   Using the Open Repository of CRISPR Screens BioGRID ORCS currently houses over ...

  6. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ....  When we started in 2004, we were the plasmid repository. In 2016, we added viral vectors, and in ...ready-to-use antibodies. Now, we think of ourselves as a repository for DNA-based materials, including our popular... kits and libraries. This DNA-based materials repository contains 139,868 reagents from 5,821 labs in ... system for scientists using materials in our repository, from our customer and science support to our...addgene.org! Fun fact! Did you know you can search for plasmids from a publication by pasting the paper's title...

  7. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...,000 CRISPR plasmids in our repository, and we have shared almost 300,000 CRISPR plasmids and 12,000 CRISPR... (A) Cumulative CRISPR plasmids shipped by year. (B) Cumulative CRISPR plasmids deposited with Addgene...these CRISPR plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing a...once made available through the repository. And the number of plasmids and libraries deposited and requested...CRISPR plasmids requested each year by year of deposit (Figure 3), we saw that once CRISPR plasmids become... CRISPR plasmids seem to never truly go out of style. Instead, an almost even mix of plasmids (by age)...Total CRISPR plasmids deposited by country; square size is proportional to number of plasmids deposited ...

  8. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    Published
    March 10, 2016, 3:30 p.m.
    ...curating plasmid collections. Addgene's "How to cite this plasmid" section Anytime you use a plasmid from ...variety of plasmids in the repository. And last, but certainly not least, we often feature new plasmid technologies...Special Collections Pages Suggest Plasmids for the Repository Find Plasmids for Your Research  ...process, we explain how to cite plasmids from Addgene right on our plasmid pages and provide a citation ...comparing two plasmids, we recommend reviewing the cited papers to determine whether the plasmid has been ...easier for you to browse through the many plasmids in the repository, our scientists have curated a number...collection or if you think a particular plasmid or type of plasmid is missing from our collections pages...

  9. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...maximize the use of both when making plasmids. Use repositories Repositories such as Addgene help share materials...pages to more easily find plasmids Search for a plasmid in Addgene’s repository ...cost required to make a plasmid. In 2023 (and at closer to 2 million plasmids shared than not!), we think...great Plasmid Debate of ’18, Blue Flame depositor John Doench calculated the time to make a plasmid. Doench... make a plasmid? Doench also calculated the cost of consumable reagents used to make a plasmid, which ... to make plasmids that already exist, and we significantly reduce costs of producing plasmids with high-throughput...lot of plasmids, it’s almost always faster to order from us!) Ask the PI If you want a plasmid for free...

  10. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...source data repositories Over the past decade or so a number of general purpose repositories have been ...value the repository integrations, because when RSpace documents are deposited into the repositories, links...research; and (3) connectivity to open source data repositories. To illustrate this, let’s take a look at the...data sources, tools used in research, and data repositories. Below I describe how each of these kinds of...apps, colony management software, and method repositories like protocols.io. These tools are essential...integration with protocols.io, the protocol sharing repository. As illustrated in this video the integration...one of five general purpose open source data repositories: Dataverse, Zenodo, Open Science Framework, ...

  11. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...bacteria with the plasmid can grow. As the bacteria divide, they replicate and retain the plasmid. This allows...Learn all about Plasmids in our Plasmids 101 series! ...Every plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the...about Developing Lab Management Software    The plasmid's journey begins First, each package is opened and... to hundreds of samples, with an average of 10 plasmids per deposit. Each week an average of 16 deposits... prepares the sample for entry into Addgene’s repository. For long-term storage in our facilities, every...allow us to store multiple types of tube for each plasmid we have at Addgene. Each sample at Addgene has ...

  12. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ... here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids sent to happy scientists around... you all the plasmids you need. We're focused on our mission – documenting your plasmids, storing them...500,000 plasmids go? Far and wide – that's where! Check out the map below which depicts where plasmids have...Sidney Street. Then the plasmids travel to labs around the world. We've shipped plasmids to more than 90,000...scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside of the... being part of our growing community! Our plasmid repository succeeds because of our amazing community...providing quality control and customer support, making plasmids easy for you to find and request in our online...

  13. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...the 879 viral vectors in our repository are produced from only 413 plasmids containing a genetic payload...conditions. Sharing our experiences Addgene’s plasmid repository gave us the experience to launch a high-quality...been finding and sharing stories unique to our repository and resources. As editor of Addgene’s eBooks ...realized we could apply the lessons learned from our plasmid lab to develop a reliable and robust high quality...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...order high quality viral vectors from the Addgene repository can save researchers significant time and removes...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...

  14. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...deposit their plasmids to the repository. These protocols show up on the related plasmid pages and can...consistently been one of the repository’s most frequently requested plasmids. I wondered how many scientists...you’re working with plasmids and you decide to share them using Addgene’s repository, you can also share... your protocols onto your plasmid page, scientists who request your plasmid will have easy access to your...restriction digest of plasmid DNA, and bacterial transformation. And more plasmid-related educational resources...be found in our Plasmids 101 blog series. Want to learn more about sharing your plasmids via Addgene? Check... resources, such as protocols, Plasmids 101 articles on various plasmid features, and reference tables...

  15. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...creating a repository that made it easy to share plasmids, both for people depositing plasmids and for people... materials in our repository. We started to increase accessibility to our repository by making it easier... Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression...Expression Systems AAV Plasmids Blogs Origin of Replication What is a Plasmid? How to Design Your... Verify Your Plasmid Using a Restriction Digest Analysis Videos What is a Plasmid? How to Design...video, however, is “What is a Plasmid?”, An animated explainer of a plasmid and its parts that helps the...incremental changes. It was only after creating a plasmid repository and shipping internationally, after all, ...

  16. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    Published
    March 16, 2016, 5:30 p.m.
    ...asking you for plasmid suggestions. Can’t find a plasmid you’re looking for in the repository? Let us know... with #suggestaplasmid, the name of the plasmid and a publication where we can find the plasmid. While...Oh! This plasmid would be perfect for my next experiment!” but were unable to find the plasmid at Addgene...here to help with our Suggest a Plasmid Campaign. How to suggest a plasmid The scientists at Addgene work... our "Suggest a Plasmid" form Fill out the form with the name of the specific plasmids you’d like to suggest...work hard to seek out plasmids to add to our collections pages, to comb through the literature for potential...depositing, we hope this campaign will expand the repository in ways that will be most useful to you, the ...

  17. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ... dug into the data from our repository to find our most requested plasmid in 2015. In line with all of...excited to announce that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR...fantastic research tool has been requested from the repository nearly 1,000 times in 2015! If you haven’t already...put to such great use. It’s just one of the many plasmids being distributed to and accelerating research...Knockouts Read Some Common Ways to Deliver CRISPR Plasmids to Mammalian Cells Resources on the Addgene Website...Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries ...

  18. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene and we distribute...opportunity for Addgene to present our own data on plasmid deposits and distirbution for these fast moving...distribute over 130,000 plasmids in 2014. Thus, we are in a unique position to observe and quantify how...scientific community. Data on CRISPR and synBio plasmid sharing In a recent review on CRISPR technology...Zhang acknowledge the role of data and materials repositories in aiding the dramatic spread of the Cas9 technology...trends for Zinc Finger, TALE, CRISPR, and SynBio plasmids over time. You can see these data in the slide...molecular biology technology? Have you deposited your plasmids yet?      ...

  19. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five different... Addgene repository. Read on to learn more.  What will be available?  Over 3000 AAV plasmids in the Addgene...Addgene repository will be available for Packaged on Request service. A small subset of AAV plasmids will...both our depositors and requestors.  Requesting Plasmids available for Packaged on Request consideration...recommend you check the original paper of your AAV plasmid of choice for more information on its performance...performance.   Titers We expect titers for plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013...

  20. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    Published
    March 10, 2015, 2:25 p.m.
    ... Bank (DSHB), directed by David R. Soll, is a repository for monoclonal antibodies, distributing over ...against new cancer biomarkers. We believe that repositories, such as Addgene and the DSHB, play an important... to ensuring affordable access to materials, repositories provide standardized quality-control and technical...about Scientific Sharing: The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years Data...

  21. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...not a party (signatory) to the MTAs for plasmids in our repository. Instead, we facilitate the signing of...organizations choose to share their plasmids under an OpenMTA, which allows plasmids to be used for both research...If you’ve deposited or requested plasmids from Addgene, then you’ve been involved in the material transfer...Even though this process is essential to sharing plasmids around the globe, it can be a bit of a mystery...use agreements. The UBMTA, which is the MTA most plasmids at Addgene are shared under, is well-understood...with the number of requests and deposits in our repository. From 2004 to 2023, we facilitated 784,795 MTAs...MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually own the majority of...

  22. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    Published
    Nov. 8, 2018, 3:47 p.m.
    ...over 65,000 plasmids in the repository, each verified by sequencing, which makes the repository a convenient...synthetic biology page Search for a plasmid Find Addgene's most popular plasmids ...been coming to the Addgene website in search of plasmids. Now, scientists are beginning to see Addgene ...most common expression system within the Addgene repository is mammalian, but the largest source of unique... recently published a tool (trained on Addgene plasmid datasets) to predict the lab-of-origin of engineered...

  23. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization... international plasmid distribution!) Chapter 8 - Opportunities to create repositories with other materials...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...

  24. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ... have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene ...cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO plasmids you'll note that those are...Actually, these two plasmids from Didier Trono's lab are our two most popular plasmids of the past 10 years...influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids from Shinya...our stride. We shipped our 100,000th plasmid. A variety of plasmids continued to be popular years after...with all the new plasmids. So far, we have distributed over 10,000 CRISPR/Cas9 plasmids to more than 3,...10 years of plasmid sharing  Interested in more facts about Addgene's 10 years of plasmid sharing? Check...

  25. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...two options: “Search Repository” and “Deposit Materials”. The “Search Repository” function is our same...! You can use a manuscript title to search for plasmids from a specific paper. If you’re ready to start...everyone’s favorite section: Tools of the Day. Our plasmid and viral vector of the day have a fresh new look...new friend: Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While those...

  26. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...in the specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories will ensure that...depositing plasmids pre-publication Find molecular biology protocols Find eBooks on plasmids, CRISPR, viral...the data can be deposited in the public on-line repository (like GitHub, Zenodo, Figshare, Triad) while ...

  27. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...have deposited their plasmids into the Addgene repository. More than 4,500 plasmids have already been deposited...Addgene, plasmids, and depositing (all while our US counterparts are still sleeping). Plasmid sharing ...in research, I developed several new plasmids, including: plasmids to study the dimerization of HLA B27... Years of Plasmid Sharing: Interested in more statistics from Addgene's 10 years of plasmid sharing? ...Check out our Top 10 Plasmid Technologies blog post Or look at our "10 Years of Plasmid Sharing" Infographic...emailed. And Addgene has been there to answer your plasmid questions. But until now, you’ve always had to ...Since 2004, Addgene has shipped more than 75,000 plasmids to more than 1,500 different institutions in Europe...

  28. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...validation carried out by the commercial repository. Repositories generally ensure cell line identity and...The oldest, largest, and most diverse of these repositories is the American Type Culture Collection aka ... between materials available from commercial repositories and the same cell line from a colleague?”. The...cells lines. Even if you get your cells from a repository like the ATCC, there are times when it is important...Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...invariably much older than those available from biorepositories leading to genotypic and phenotypic artefacts...easier to conduct high quality cell culture. Biorepositories around the world ensure that the cell lines...

  29. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...organizations in 41 countries enriched our repository with 15,020 new plasmids — a testament to the scientific community's...vector options. Almost any AAV transfer plasmid in our repository can now be packaged on demand in five ...accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs...next-generation sequencing, setting new standards for plasmid quality control. Through our partnership with the...toward giving you more choice in how you receive plasmids — as DNA or bacterial stabs — ‌and reinventing...sequences and characterization data for over 150,000 plasmids, empowering bioinformaticians, data scientists...developers to unlock the potential of our extensive plasmid database — because we believe that innovation flourishes...

  30. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...this field, we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource... the Addgene repository (like these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier... of researchers who weren’t aware of Addgene’s plasmid sharing services, as well as #happydepositors who..., it looks like there are plenty more exciting plasmid tools on the horizon for microbiology research ... distribution service! Did you see a must-have plasmid during ASM? Let us know what’s missing in the collection... collection by filling out our Suggest a Plasmid Form. We look forward to helping microbiologists from...Microbiology Resource Page Deposit Your Microbiology Plasmids with Addgene ...

  31. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...Engineering While the vast majority of CRISPR plasmids in our repository use Cas9 to cut DNA, Addgene Science ...CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help thinking about the types ...Scientists eBook Download the Plasmids 101 3rd Ed. eBook Read the Most Recent Plasmids 101, CRISPR 101, and Career... two eBooks (Management for Scientists and the Plasmids 101 2nd ed, the 3rd edition has now been released...top of the many developments in science and the plasmids, technologies, and researchers behind them. As...most popular posts continue to be those from our Plasmids 101 and CRISPR 101 series. These posts aim to ... the background they need to get started using plasmids and the revolutionary CRISPR technology in their...

  32. Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!

    Type
    Blog Post
    Published
    April 8, 2021, 7:25 p.m.
    ...100,000 different plasmids available from the repository (currently at 98,276 plasmids as of April 8, 2021...re now sharing over 100,000 reagents including plasmids, ready-to-use viral preps, pooled libraries, kits... 2021) 1.5 million plasmids shared in total (currently at 1,484,507 as of April 8, 2021) 5,000 depositing...addgene.org homepage! You can track the number of plasmids right below the search bar and more stats further...

  33. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles... CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...EasyTag workflow combines thoughtfully designed plasmid components with Fluorescence Activated Cell Sorting...available from Addgene, head over to our CRISPR Plasmids and Resources page. Here are some highlights from the...Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use viral preps....

  34. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles... CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell populations...populations without end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter...available from Addgene, head over to our CRISPR Plasmids and Resources page. Here are some highlights from the...Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use viral preps....

  35. Hot Plasmids Podcast Episode 3: Optogenetics Tools, Improved CRISPR Base Editors, & More!

    Type
    Blog Post
    Published
    Oct. 25, 2017, 1 p.m.
    ...additional hot plasmids in our newsletter or on our hot plasmids webpage.   Hot plasmids covered in this...Segmennt of Improved CRISPR Base Editors Hot Plasmid 4: pPSU Plasmids for generating DNA molecular weight markers...Check Out All of Our Hot Plasmids Articles Browse the Repository  ...In the third episode of our Hot Plasmids podcast series, you'll learn about optogenetics tools for controlling...this segment Hot Plasmid 1: Photocleaveable PhoCl protein for controlling molecular processes with light.... Listen to Our Segement on PhoCl Segment  Hot Plasmid 2: Ouabain selection for increased CRISPR editing...Listen to the Segement on Ouabain Selection  Hot Plasmid 3: CRISPR base editors with enhanced range and ...

  36. Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

    Type
    Blog Post
    Published
    Dec. 11, 2017, 2:02 p.m.
    ... this Hot Plasmids episode! Hot plasmids covered in this segment Hot Plasmid 1: B. subtilis knock out ... find additional hot plasmids in our quarterly newsletter or on our hot plasmids webpage. Listen to this...Check Out All of Our Hot Plasmids Articles Browse the Repository  ...In the fourth episode of our Hot Plasmids podcast series, you'll learn about new libraries for studying...studying B. subtilis biology, plasmids for enhancing iPSC production, and CRISPR tools for plants. You can... Our segment on the B. subtilis libraries. Hot Plasmid 2: Transcription factor fusions modulate iPSC reprogramming... segment on modulating iPSC reprogramming  Hot Plasmid 3: Regulating telomere length with TZAP. Listen...

  37. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    Published
    Sept. 8, 2017, 7:52 p.m.
    ...hot plasmids in our newsletter or on our hot plasmids webpage. Listen to the entire Hot Plasmids podcast...podcast! Hot plasmids covered in this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly...Newsletter Check Out All of Our Hot Plasmids Articles Browse the Repository  ...In the second episode of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins...Segement on the Tol 2 Gateway-Compatible Toolbox  Hot Plasmid 2: AAV Vectors for tageting GABA-ergic interneurons...Listen to Our Segment on these new AAV Vectors Hot Plasmid 3: mCyRFP1 - a new, monomeric, and cyan-excitable... protein. Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent...

  38. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... sharing plasmids (with 16 labs currently sharing viral preps) through the Addgene repository. Without...relevant plasmid depositors and their institutions to distribute viral preps derived from plasmids in the...largest new initiative since we started distributing plasmids in 2004. We’ve already distributed over 500 viral... virus. Addgene is a 501(c)3 nonprofit and our plasmid service was started based on donations. That seed...positive response to the option of Addgene providing plasmids in a ready-to-use viral format. Having identified.... With their expertise in shipping hundreds of plasmids a day, the Lab and Office Teams develop shipping...the repository. The Outreach Team has been making sure scientists know about the new initiative and serving...

  39. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...receive transfer plasmid DNA that you can use along with lentiviral packaging plasmids to create your own...rough approximations of the total number of CRISPR plasmids for that particular organism available at Addgene...helped distribute many of these technologies in plasmid form and are proud to have facilitated their fast...fast adoption. However, in many cases the plasmids themselves are only the starting point for the production...lentiviral preps of select lentiviral vectors from the repository for you. We perform test infections with all ...lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA libraries that can be ... use; after receiving a pooled gRNA library in plasmid format, a researcher must amplify the library, ...

  40. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...money producing virus from select vectors in the repository yourself, you can now order ready-to-use virus...accelerate your research.   Why distribute virus? Plasmid distribution is booming, but, over the years, many...list of what’s available or suggest additional plasmids with the Viral Service Suggestion Form. Lentivirus...of the most popular lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting...
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