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We narrowed to 812 results for: APP

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  1. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ... FREE science lab apps that have been educational and great for lab life. These apps prevent you from ...spectrometry app. I digress. When I started learning proper cell culture technique, I tried to find an app that...I came across the Gibco Cell Culture app. Gibco organizes its app into four categories: tools, analytics... Tools app comes in handy when I use protocols that require restriction enzymes or PCR. The app contains...Hence, I’m including this MSDS app. The Chemical Safety Data Sheets app has a search function that allows...of the many apps available to help researchers in the lab. If you have a favorite lab app, please share...by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article are those of the guest ...

  2. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...previous nosy question! The Application Process Broadly speaking, the PhD application process tends to go something...steps in the application process and usually occurs in the few months leading up to applications opening. ...program. Most applications will recommend that you name two professors in your application that you intend...to continue the application process through a campus visit, a weekend where applications can get to know...strengthen your application and help you decide if a program is a good fit for you. The application process looks...if you’re anything like me, the process of even applying to one of these programs is almost as daunting...be, at times, very overwhelming. As someone who applied to PhD programs in Biology around the United States...

  3. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...technique published every week! One of the newest applications is a colorful system that uses fluorescently...Thoru Pederson’s lab, brings us one step closer to mapping the 4D nucleome, the organization of the nucleus...activity, catalytically dead Cas9 (dCas9) has many applications, the most well-known being transcriptional activation... 9 and 13. They next turned their attention to mapping pairs of intrachromosomal loci. The technique successfully...authors’ knowledge, this work represents the first mapping of intrachromosomal loci, a major benchmark in ...characterizing the 4D nucleome. Future modifications and applications Ma et al. developed this technique using standard...for this technique. The NIH Common Fund has made mapping the 4D nucleome a specific priority, as researchers...

  4. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...exhaustive list of all the apps out there, we encourage you to mention any apps you’ve found useful in the... other posts that deserve a second look. 5 Great Apps for Lab Life This very useful post was contributed...provides a brief rundown of Sean’s top five smartphone apps you can use in the lab. Whether you’re tired of ...that chemical in your obscure growth medium, these apps can help make your lab life a bit easier. Since ...

  5. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...sure I missed some of your favorite apps. If you know of a software app that I missed, let me know in the... The software can be used with the ZappyLabs Bench Tools mobile app (available for iOS and Android). A...system does not appear to have a way to share your protocols with other groups. What apps do you use to ...publishers, and small start-ups are turning to software apps to solve our need for quicker, more effective updating...more to fit every lab's needs. Plus there are the apps - many to help you access your protocols at the ...comments below and leave your thoughts on how the app works for your lab’s needs. At Addgene, we’ll continue... some of our basic protocols to the protocols.io app so that all of our researchers can easily take those...

  6. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...their use in other clinical applications. In the last decade, viral applications in cancer treatment and ...s unpack the myriad number of emerging viral applications and give you some recommendations for putting...putting viruses to work. Viral applications Viruses work by infecting a host cell (the target of viral infection...expressed. It’s important to note that for all the applications we discuss, there is more than one type of virus...retrovirus, AAV, etc.). When you’ve decided on an application, our Viruses as Biological Tools blog can help...viruses and pick one for your experiment. Research applications Gene expression control Researchers are always...becoming increasingly popular tools with many applications. These libraries include sgRNAs targeting the...

  7. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...have been developed for different scientific applications. These PCR methods all use the same basic PCR...quantified by qPCR. qPCR is used in a variety of applications including gene expression profiling, studying...that fractions the PCR reaction sample into approximately 20,000 droplets. Each droplet contains the material...

  8. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...function (Wood, et al). In the microbial world, approximately 50% of predicted genes have unidentified roles...endogenous gene. Each trap uses a slightly different approach (described in detail below), but fundamentally...redundant genes – multiple genes which function in overlapping roles can mask the depletion of one another. ...trap systems lack a promoter and thus require the trapping of an endogenous promoter. The system is composed...enhancer traps lack enhancer elements and require the trapping of endogenous enhancers to stimulate the expression...many more! Check out Addgene’s catalog to find trapping tools for your next experiment! References and...

  9. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...and applications.    Plant applications We outlined many synthetic biology MoClo applications, but...for synthetic biology applications in bacteria or cell-free systems. The approach is particularly useful...- and multi-gene constructs for a variety of applications. MoClo tools can be used in bacterial and cell-free...and the kits Addgene offers to kickstart these applications, no matter what your model organism is. MoClo...this blog, we will highlight some common MoClo applications and kits. Keep in mind that this technology ... it, MoClo might help you achieve it! MoClo applications and specialized tools Synthetic biology in bacteria...effectiveness. Perhaps the most intuitive CRISPR MoClo application is to assemble multiple unique sgRNAs into a ...

  10. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...options, you’re likely to find an application that matches your needs. Happy quantifying!  References and...blog post, we're going to focus on PCR-based applications (qPCR and ddPCR.) qPCR Quantitative/real-time...range of RQ values as noted above.   In both applications, results can be affected by DNA quality and ...as positive or negative for each reaction, math happens (this time, Poisson statistics), and the readout... triplicates. ddPCR is an extremely accurate application, even in areas of the genome that are difficult...experiments, a number of factors will determine which approach is right for your experiment. You’ll have to consider...QuantificationPolymerase Chain Reaction Overview and Applications Resources on Addgene.org Protocol: Polymerase...

  11. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...particular application can be found here.  Why CRISPRa? Although cDNA overexpression approaches may still... viral vectors, and high-throughput screening approaches. Here, we’ll highlight these recent advancements...advancements and offer commentary on their application to neuroscience research. What is CRISPR-based activation... a larger packaging capacity than AAV. Such an approach was described recently by the Day Lab, who developed...accessible to neuroscientists, not all neuroscience applications require such optimized tools. This is elegantly... developed a subgenome-scale CRISPRa screening approach in embryonic stem (ES) cells to determine which...study neuronal differentiation, it could also be applied to the differentiation of other CNS cell types,...

  13. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...The Framework for In vivo T cell Screens (FITS) approach (Milling and Markson et al., 2024), developed ...compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker to facilitate... et al., 2024.   Proof of concept The FITS approach was used to optimize conditions for a screen that...

  14. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...protein for your application. Selecting the best fluorescent protein for a specific application can seem like...under conditions that best mimic the intended application. The three key properties for live cell imaging...theoretical brightness. The theoretical brightness often appears in tables with fluorescent protein properties and...experimental set up. The practical brightness takes all application specific parameters into account, including the... picture of what can be expected in a 'real' application than the theoretical brightness. Consequently...brightness in a system closely mimicking the future application. In figure 2 we provide an example of the comparison...

  15. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...explore alternate approaches. Being a “lazy” geneticist, I developed a co-selection approach relying on repair...with unprecedented ease. These new C. elegans approaches rapidly enrich for editing events without the...phenotypes (unc-119) (Dickinson et al., 2013). These approaches allowed effective recovery of knock-ins, but ...Including Cre-mediated marker excision, it takes approximately 3-4 weeks to obtain a homozygous, outcrossed...several manuscripts published in Genetics detailed approaches in which selection for an editing event that ...and knock-ins at other genomic loci. The first approach – co-CRISPR from Craig Mello’s lab – used inactivation...animals with particular visible phenotypes. These approaches may become even more powerful following a report...

  16. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...cas9 gene and single guide RNAs (sgRNAs) with appropriate flanking homology into a germline cell can create...RNA-guided gene drive. In principle, this event could happen even if there isn't any homology available. Consequently...local biosafety officer should be familiar with appropriate measures. Barriers should be a component of all...eventual release should consider building an appropriate immunizing reversal drive at the same time to...species in question), why not use them whenever applicable?Against this, consider the cost of an accidental...An accidental release could delay real-world applications against scourges such as malaria and dengue .... Disclaimer: Recommendations concerning the appropriate degree of confinement are those of the author...

  17. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ... the app. Technical difficulties? Attending a conference virtually means getting to know the app or virtual...conferences with multiple sessions happening at once, virtual conferences may have apps or interactive calendars...was able to get in touch with the author. “In the app, you can write a message to the author of the poster...there may be a “place” for networking within the app. For example, the NeuroSymposium had virtual tables... puts butterflies in your stomach, the virtual approach might be less stressful. For both conferences,...Having an on-site conference canceled may be disappointing, but there are many creative ways to adapt and...

  18. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells...based affinity purification and mass spectrometry approaches. Recently however, scientists have developed ...miniturbo Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-... PMC3308701. Rhee, Hyun-Woo, et al. "Proteomic mapping of mitochondria in living cells via spatially restricted..., et al. "Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally...proximity-based labeling methods for interactome mapping. F1000Research, 8.  ...

  19. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...for your needs 5 great apps for lab life  Sharing your lab protocols using apps to save you time and track...sign-in required.)  DailyCalcs Science Calculator app for iPhone Reagent and Data Repositories These links...paste into other applications Primer design tools Many of the above-listed plasmid mapping tools have built-in...Primer design. Plasmid mapping. DNA sequence analysis. We all have our favorite tools for tackling these...molecular biology tools all in one place. Plasmid mapping These tools are for viewing, editing or making ...the paid version provides more tools for plasmid mapping and design (Figure 1). Benchling: While you might... desktop-based software for plasmid design and mapping. ApE (A plasmid Editor): A free, donation-based...

  20. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...starting a writing group. Loop Habit Tracker app for Android  This app lets you track habits, like writing. It...reinforcement for a habit. The best part of this app is it turns your writing habit into beautiful graphs...

  21. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...neurodegeneration research: Anti-MAPT [AT8] and Anti-APP [6E10-A5]. Both are recombinant mouse monoclonal ...Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant antibody in human postmortem... antibodies here! Wu, Z., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene...in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots. But Jae-Yean...efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024). Their optimized strategy uses... wild type (WT), two weeks after herbicide was applied to both plants. Adapted from Vu et al., 2024 bioRxiv...CC-BY-NC-ND 4.0 International license. With this approach, the team was able to drive prime editing in both...

  22. The Twelve Days of Antibodies

    Type
    Blog Post
    Published
    Jan. 6, 2025, 5:17 p.m.
    ...Antibody, Addgene gave to me…  A recombinant Anti-APP!   🎶On the eleventh day of Antibody, Addgene gave...

  23. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...plan out your agenda. Some conferences even have apps you can use to build a schedule. If there is a list...meet. Some conferences may also have a dedicated app with a social media component, but if it doesn’t ...up, create one yourself. Pick a time (ex: lunch, happy hour) and use the conference hashtag to set one ... this, you probably notice a trend: networking happens everywhere! Even when you aren’t “trying to network...

  24. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...Save Money with the Baby Gel Box Find 5 Smart Phone Apps for Lab Life Learn About the Evolution of Lab Techniques...

  25. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...other tools they use in research. This includes chat apps like Slack, Microsoft Teams, and Google Hangouts...and science-specific tools, e.g. sample management apps, colony management software, and method repositories... take a look at the RSpace ELN.  The connected approach in action: how the RSpace ELN supports reproducibility...

  26. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...Finally, my main content organizer is the amazing app Evernote. I have a paid account which gives me all...for Scientists Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments Additional...to find a way to keep doing science to be happy. The happy path is not the academic one for the majority...say that doing a Reddit “Ask Me Anything” was appealing, but also scary. I do a lot of public speaking...leaving out the term “implicit bias” which is apparently a troll trigger phrase these days). Redditors...database. Still have questions? I am easy to reach and happy to help. Additional Resources on the Addgene Blog...

  28. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ... carry a notebook at all times (or use the notes app on your phone) to capture fleeting thoughts and connections... your intellectual journey. Practical approaches One approach to achieve this goal could involve participating...often happen when we allow ourselves moments of release and mental freedom. Practical approaches Engaging...predefined path, opening doors to novel ideas and approaches. Moreover, the ability to embrace serendipity....” This quote encourages us to contemplate our approach to life and how our viewpoints influence our encounters...

  29. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...something else to focus on. Use collaborative cloud apps It is a good idea to use collaborative cloud tools...miscommunication, a lack of leadership, and inappropriate tools or writing approaches can lead to frustration, delay...hands or signal the approach of a brain break, where everyone stands up, disappears from the video, and...members talk no science. No data presentation will appear here, and only general chatter will be allowed ...social distancing. The take home message is the approaches/tools we have to foster creative collaboration...

  30. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ... you are interested in.     STEAMid is an app that helps you find internships and scholarships ...student from applying to the opportunities of their dream.  To help students focus on the application process...internships you are applying to, keep your resume under two pages unless you are applying for a Federal Government...your application a whole week before the deadline. The day of submission, review your application with...the resource, and apply as a cohort. This may sound counterintuitive since that approach seems to create...Scrutinize every opportunity prior to starting an application to assess your eligibility, interest, and career...February represent peak season for internship applications. Thus these noble individuals are inundated ...

  31. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...invitation. Don’t try to connect from the LinkedIn phone app either as this also results in a generic invitation...out right. Also, many people on LinkedIn don’t appreciate random requests without a personal message and...as spam or “I Don’t Know This Person.” If that happens a few times, LinkedIn may revoke your ability to...post and occasionally comment on their posts as appropriate, possibly giving you a way to connect with them...updates, or click bait of any kind. For more on inappropriate content see this article. However, do comment...

  32. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...Notebook for Bioinformatic Analyses Use Software Apps to Save Time and Track Your Experiments Additional...Find the appropriate glycerol stock within our freezers Pick the sample into the appropriate stab culture...corresponding entry in your database. Enjoy your happy lab staff. Barcoded materials make everyone's life... is almost always worth it. Because labels are applied to containers and not the mushy, gooey contents...be!) bear in mind that your barcodes are being applied to the containers, not to the contents. Tightly...contents of a tube with the tube itself. If something happens to the worm that requires a change of tube, there...

  33. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...section.   Additional Resources on the Addgene Blog Apps for Lab Life Developing Lab Management Software ...

  34. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...titles and abstracts are available online or in an app prior to the session, so bookmark which posters are...background without an introduction. If someone approaches your poster, introduce yourself and ask about... a related topic, and let them know while you appreciate the thought-provoking question, you are unable... enjoy hearing the highlights so that you can appreciate the impact of the project as a whole. In addition...

  35. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ... scientists––CrispyCrunch! CrispyCrunch is a web app that helps scientists design and analyze batches ...score is less than 0.03, it stops mutating. (This happens often because the HDR inserted sequence splits ...Psychology at McMaster University in Canada. He's happy to be contributing again to science.   Acknowledgements...

  36. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...letters to be very important. On the other hand, our app/dev team rarely, if ever, sees cover letters submitted...usually yes! A good cover letter won’t hurt your application, but it can certainly help you stand out.  In...consider cover letters an important part of the application. Even within an organization, there can be variance...good cover letter!) As with any part of the job application process, it's a good idea to talk to people in...do the job as described. Let’s imagine you’re applying to a job that requires ddPCR skills. Your resume... to proactively address questions about your application. These could be logistical, like briefly explaining...  Finally, it’s a good idea to state why you’re applying to the specific company or lab. Take some time...

  37. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...Learn about more tools for reproducibility 5 great apps for lab life Developing Lab Management Software ...sciNote. Today, every lab has its own habits and approaches to record keeping. Top priorities in most cases...Project: Cancer Biology have shown similarly disappointing rates of reproducibility. These alarming results...familiar paper-based lab notebook is still a standard approach to record-keeping in many labs. In a way, it will...digital data generated in labs. While many labs approach the situation differently, some adopting simple...might be available as well. In most cases this applies to universities. How are you currently exchanging... Besides this being of crucial importance when applying for a patent for example, it also comes in handy...

  38. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...mutations in genes associated with dementia, such as APP and PSEN1, in most cases the intended HDR events ...with blocking mutations, we still weren’t entirely happy with our editing outcomes and in many cases still...

  39. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...tools as some kind of a meta/Frankenstein app? Or perhaps there is a clear winner, is there something that...the one hand we have software tools that apply some approximate or sensible rules to determine the value... more efficient.  These insights are hard to appreciate without computational support. Indeed, rational... solutions that facilitate most experimental applications in CRISPR engineering. When compared to that...easy to fault the lack of features in a single application. The question is then, should we use all tools...2013) used a combination of both empirical and approximate methods. The details of their scoring function...and it means that there is no one-size-fits-all approach. You will have to select a tool based on your ...

  40. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...knockout approaches. In these cases, we would recommend a knockdown approach instead. In this approach, you...using one or more of the approaches outlined below. Knockout cell line approach Genetic knockout-based...Knockdown approach For cell types where knockout studies are not feasible, an alternative approach is RNA... the target of interest. This approach maps onto the orthogonal approach within the five pillars and is...knockout/ or knockdown approach should be preferred. The overexpression approach is therefore best suited... use of orthogonal approaches, cell treatments, and independent antibody approaches in different assay...target-of-interest in your assay and sample-of-interest. The approaches available broadly map onto the five pillars of...

  41. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...assume that their advisor alone applies for these competitive grant applications; however, in some cases, your...the grant application process. When my PhD advisor first asked me to write a grant application due the ...mind that universities require application submission in-house approximately 2 weeks before the RFA due date...the next round of applications. Step 5: Keep writing! Each written grant application builds upon the ... project grant application" by the NIH Still want more information on the application process? What types...writing, volunteered to take the lead on various applications throughout my graduate career, and helped receive...opportunities to convince others why a project is the best approach to a given problem, Empowers the writer, And...

  42. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...intended application - Remember, just because your antibody performs as expected in one application does ...Strategies This approach compares target expression of antibody-independent approaches to your antibody-dependent...Genetic Strategies This approach uses a gene knock-down or knock-out approach to show that an antibody...protein. This approach is ideal for validating antibodies for immunocapture applications and is amenable...pause and make sure that your antibody has been appropriately validated so that you can have confidence in...in your results. But what is appropriately validated? This post will go over some of the general considerations...strategies for antibody validation in different applications. Here, I want to highlight the general suggestions...

  43. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...allow you to approach that problem in a novel way. Finally, engineering new applications to solve problems...good quality application. Even following this advice, only about 50% of my grant applications have been ...do need to be able to research and write your application, then give it to someone else to read and / or...it). It’s easy for reviewers to spot when an application is rushed (whether the idea isn’t properly researched...researched / developed or the application is full of typos) and these are unlikely to get past the initial...someone else in the department) to read over your application and give you feedback. They’ve most likely been...alterations before submitting - handing them your application 3 days before the deadline isn’t going to be ...

  44. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...of four split AAV vector approaches. 1. Overlapping  Overview: The overlapping strategy has a region of...directed repairs (HDR). Split AAV vector approaches Split vector approaches increase the size of the gene delivered...  3. Hybrid  Overview: The hybrid approach combines the overlapping and trans-splicing methods. The AAV...has a slight advantage over the overlapping and trans-splicing approaches since there are two ways for a... genomes is the basis of several split vector approaches developed in the early 2000’s which allow researchers...is not yet known. Drawbacks:  Generally an overlapping split vector has equal or higher transgene expression... on the rate of transgene expression with an overlapping AAV since both of these variables influence whether...

  45. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...is this application - the creation of sequence-specific knockins - for which the AAV approach is most ...for a given experimental approach. First, unlike CRISPR, the AAV-based approach is only able to target ...simultaneously in the same cell, are more appropriate for a CRISPR-based approach. Second, the AAV preps generally...limitations, the AAV approach is best suited for tissue culture based approaches that require modification...researchers could apply this technique to efficiently deliver large repair templates for applications that require...working to develop high efficiency gene targeting approaches improved on this finding with the addition of...challenging to develop high efficiency CRISPR-based approaches for the generation of knockins via homologous...

  46. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    .... This is the direct approach to immunoimaging assays. But if the direct approach isn’t giving you enough...   Figure 1: The direct approach (left) versus the indirect approach (right). Primary antibodies ...varies across applications. Western blots, for instance, almost always use the indirect approach, while flow...immunocytochemistry may all use either the direct approach or indirect approach, depending on the requirements of the...interest, it may be time to look at the indirect approach - which means it’s time to talk about secondary...binds to the primary antibody. In the indirect approach, the signaling molecule is conjugated to the secondary...antibodies can bind to a single primary antibody, this approach can greatly increase the sensitivity of an assay...

  47. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ..." are summarized below. Check Out Erik Snapp's Full "Applying for a Faculty Position" eBook Here 1. Have...  Erik Snapp just released version 3.4 of his eBook, How to Get a Faculty Position. This edition has ...along with new thoughts on research statements. Snapp, who is always looking for ways to improve his eBook...suggestions, please do not hesitate to email me at snappe@janelia.hhmi.org." You can find his original blog...years ago, I decided to write a "how to" manual on applying for faculty positions in biomedical science. My...talents and ideas. Some key points from my ebook, "Applying for a Faculty Position: the View from Both Sides...experts in your field. Not true. Even if you are applying to a department in your specialty area, it's a...

  48. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...safe for clinical applications. Excisable Cre-lox lentiviral vectors: This approach is the same as using...of excision, this approach is still not considered safe for therapeutic applications. piggyBac (PB) transposon...This is considered a safer approach for developing therapeutic applications, but it can take a long time...time, scientists have used almost all available approaches for generating iPSCs. The generation of iPSCs...Retroviruses are not considered safe for clinical applications.     Lentiviral: Lentiviral vectors infect dividing...dilution via cell division. A drawback to this approach is it has lower levels of efficiency at generating...express reprogramming factors for therapeutic applications. Sendai viral vectors: Sendai virus is a single...

  49. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...relationships. However, most job seekers will and should apply for jobs posted online as one part of their job ...Utilizing existing relationships as part of the job application process can dramatically increase the chances... of a resume can be crucial for a successful application. It takes time to prepare an excellent resume... Many job postings will result in hundreds of applicants the day they are posted.  If your resume is not... here for more on timely submission of a job application. 3) Read job descriptions carefully Be open minded...have to have all of the listed qualifications to apply for a position.  A job description is usually a ...wish list and the hiring manager knows that no applicant is likely to meet all the criteria. Note the criteria...

  50. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...skipping approach in mice, DMD researchers are very excited. This approach may also be applicable to a number...success in basic research, researchers are eager to apply it in a clinical setting. CRISPR is often used for...), an X-linked recessive disorder affecting approximately 1 in 5000 males. DMD is caused primarily by ...very large (79 exons), but much of the sequence appears to be nonessential. Within the mutational hotspot...so researchers have set their sights on another approach. Since shorter forms of dystrophin can still be...researchers have begun to explore genome editing approaches for exon skipping. Addgene depositor Charles ... removal rate of exon 51, which resulted in appropriately localized dystrophin. In a subsequent study,...
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