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We narrowed to 610 results for: STO;

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  1. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...tend to have a story structure: beginning, end; cause, effect; lesson, learning. Storytelling is a crucial...even tell short stories during weekly lab meetings. When scientists tell good stories, they engage their...appreciation for their research. Long story short, telling a good story is something that can be learned, ... your career. Story Telling at work Don’t believe me? Here’s an example of storytelling to answer an informational...conclusion. Advice for Telling a Good Story Start at the start Any good story needs a solid point to kick off...of versions of your story and find the one that fits your audience. Enjoy the story you’re telling There... form of a story, and you’ll very quickly get better at telling it. End at the end A story needs a good...

  2. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...with Tim Miles: While not technically a research story, I couldn't resist adding in this excellent Q&A ...try in your own work!) If you've got a research story you want to share on our blog, please reach out ...

  3. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...are doing is telling a (nonfiction) story. For there to be a story, you need to have conflict. There are...complete your story: an introduction, and a resolution.   Fig. 2: The structure of a story - not just...unrelated stories that they then tuck the science into. There are almost limitless storytelling options,...pursuit of achieving knowledge. Or, as we in the storytelling business like to say, science is all about navigating...inherent tension, is interesting. It’s what drives a story forward - and this drive is so powerful that it ... communications, but they’re firmly part of the story to the scientists doing the work.  And while these...illustrates the value of the work and then build a story around them. A good rule of thumb is to find the...

  4. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...Pre-CRISPR  Having visualized and understood the DNA, the next milestone for the molecular biologist was...Ridley Scott directed Blade Runner (1982). It is a dystopian film that revolves around synthetic humans called...year after the discovery of meganucleases. The storyline involves the resurrection of dinosaurs by extracting...more blog posts about CRISPR Learn more about the history of CRISPR Read about other genome editing methods...200304010-00015 Wood AJ, Lo T-W, Zeitler B, Pickle CS, Ralston EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X...

  5. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ....  Storage   Once you get your exciting new antibody, the second thing you’ll want to do is store it ...what temperature to store your antibodies at, but it also matters what they’re stored in - their buffer....Resources References Boston University Flow Core. Fluorochromes Brightness Chart. Boston University Flow Core...as well. In this post, we’ll touch on antibody storage, buffer considerations, and give just the lightest...the way, you can put your antibody in short term storage (think days to weeks) in the 4°C. If you’ll only...primarily used in Western blots and ELISAs, cannot be stored in sodium azide at all, as it inhibits HRP. In ...sensitive, so antibodies conjugated to them need to be stored either in a dark, opaque vial or wrapped in aluminum...

  6. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...Addgene is announcing we have chosen to stop all activity on X, formerly Twitter. If you are interested...posting on November 20, 2023, and as of today, will stop posting, monitoring, and resharing content on X....

  7. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...futureofresearch.org/boston/. The Future of Research Symposium A year ago, trainees from around Boston met to discuss...BEST programs, including local schools in Boston: the Boston University School of Medicine and the University...The second Boston Symposium on the Future of Research will be held from 22-24 October. This blog has ... to be held at the Future of Research Symposium Boston 2015 – Academic Data and the Labor Market. For ...week, postdocs and students will again gather in Boston - this time with the aim of finding solutions to...great panels at the Future of Research Symposium Boston 2015 where we hope to begin developing concrete...participate in the Future or Research Symposium Boston 2015 you can register here or live stream the event...

  8. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...science stories. Here are some of our favorite spooky science stories. Six spooky science stories Zombie...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways ...

  9. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...efforts is the Golden Stopwatch Award to celebrate exemplary TTOs. Our new Golden Stopwatch Award recognizes...certain rights and protections for depositor and requestor alike. That’s why Addgene works closely with TTOs...agreement (usually) must be executed. The Golden Stopwatch Award highlights TTOs that accelerate science ...agreements quickly! If your TTO receives a Golden Stopwatch, consider sending an email to thank them--after...examples if possible Tell them about the Golden Stopwatch! Any organization that deposits with and/or [email protected] Congratulations to the Golden Stopwatch awardees! Congratulations to all of the winners...College of Medicine  Baylor College of Medicine Boston Children's Hospital  Brandeis University  Broad...

  10. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...a Lox-Stop cassette, resulting in the neurological deficits observed in Rett Syndrome. Restoring the MECP2...to Rett Syndrome, this blog post describes the history of Rett Syndrome research, the role of the MECP2... MECP2 gene by excision of the Stop cassette using tamoxifen-inducible Cre not only halted the neurological...precise amount of protein to each brain cell to restore physiological levels. Much research is now focused...

  12. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...is a new software tool used to design gRNAs for custom CRISPR screens targeting a (long or short) list...Glance!  CRISPR knockout screens: Full genome vs. custom screens Full genome CRISPR knockout screens have...hypothesis driven research question. Examples of custom CRISPR screens Differentially expressed genes  ...massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target differentially...microbe or a cytokine is added to a cell). With a custom CRISPR screen and the appropriate phenotypic readout...surface proteins. One of the first challenges with a custom screen approach is to identify an appropriate list...the Output_Short file directly. We have used CustomArray, Inc for this purpose.  The gRNA oligos are...

  13. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally participated as a ... Research Symposium On the FOR website, the FOR Boston 2015 organizers indicate that the main question...information gathered by Postdoctoral Associations around Boston - From Panel Session 2: Defining the Postdoc: An... Career Path Panel Two of the panels at the FOR Boston 2015 symposium, the Career Path Panel and the Early...type of failure. Final thoughts The FOR Symposium Boston 2015 shined a light on some of the many problems... Resources at Addgene  Read David Riglar's FOR Boston 2015 Blog Post Read Our Career Blog Posts Read ...

  15. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...These are stormy times. It feels like we’re being buffeted by wind and waves and sometimes wonder if ...would be fully open and available to industry requestors as well. This is a time when we need as much ...Initiative was able to step in to be our “port in the storm.”  "Addgene is a unique resource for scientists ...whatever lies ahead this year. We can see signs of the storm passing. We’ve recently piloted Addgene’s new cloning...

  16. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...Addgene’s customer service philosophy Customer service can be a hassle. You finally make it through a...of our core values here is to provide unmatched customer support, as this is vital to ensuring that our...technical, or quality control issues. Improving customer support with self service We strive to make our...your busy schedule, you may not have the chance to stop what you’re doing and make a phone call or write...waiting for an answer. Luckily, there’s a trend in customer support to address this exact problem, and it’... customer support...

  17. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...Learn more about the history of plasmids and cloning: CSHL Meeting: Plasmids: History & Biology The Joshua...different experiments. Addgene was founded in order to store, QC, curate, and distribute them all in the name...eBook! In keeping with our goal of creating a one-stop reference guide for plasmids we've updated and added... history of plasmids...

  18. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...'s been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings...course of the day. His talk, which focused on the history of genome engineering from ZFNs through TALENs ...synthetic biology. I've been answering biology customer service questions for nearly 7 years, and my colleagues...represented engineering first and foremost, from the custom software used to design complex genetic circuitry... of the book on my way to the meeting, and its history of code making and code breaking is wonderfully...increasingly important field.  Interested in Addgene's Keystone Poster?  We'll be sharing the poster with our...

  19. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...sell at farmers markets), and serious ideas (how to stop rhino horn poaching using biotechnology) were thrown... we decided to make education and outreach a cornerstone of our business, because we are building this...excited to see what the next few months have in store for us. If you’re thinking about making the switch...

  20. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...during storage over prolonged periods of time, especially at room temperature or at 4°C. Storing DNA: temperature...way to store and elute the DNA. References Murakami M (2013) Evaluation of DNA Plasmid Storage Conditions...and it will impact the time you will be able to store your sample without having substantial degradation...things when you elute your DNA: where you plan to store it and for how long. The most common solution is...

  25. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...Therapy. Existing methods for AAV quality control Historically, AAV vector QC has been sparse including only...contaminating AAV DNA. In addition, we wrote an open-source custom Python script that uses the VGS data to determine...data in minutes and the scripts  can be easily customized to detect other sequences of interest.   While...

  26. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...lung, the heart, and the abdomen. Long story short, when asbestos is breathed in (or less frequently, ingested...small, hook-like shape of asbestos fibers. Over time these embedded asbestos fibers cause inflammation...known safe level of exposure to asbestos – i.e., any exposure to asbestos is potentially capable of causing...exposed to asbestos but have not developed the cancer yet Those who have not been exposed to asbestos Tests...greatly decreased through a ban on asbestos. Nonprofits like the Asbestos Disease Awareness Organization ...bill passes, it will put an immediate stop to the use of asbestos, thus ensuring that future generations...completely preventable. Mainly caused by exposure to asbestos, mesothelioma is a form of cancer that develops...

  27. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...Having spent that long in customer service, I've daydreamed about the ideal customer service interaction -...to any biology-related customer support interaction. Before contacting customer service In an age of Tweeting...answered hundreds of phone calls and thousands of customer service emails in my six years as a senior scientist... - one that gives our customers the most utility in the least amount of time. Though I now spend far less...my years of accumulated wisdom so that you, the customer, can get the most out of your email or phone call... bad product (in which case, you should probably stop using that company). Frequently asked question pages...section are really, truly frequently asked by customers (and I expect this is the case at most companies...

  28. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...pH. We compared the dry ice-stored samples to controls that were only stored in a -80°C freezer and not...of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results showed that...mitigating the pH effects of dry ice storage. To do this, we stored samples on dry ice for 72 hours and...reading a publication describing how short term storage on dry ice can shift sample pH, we wondered whether...freezer and then moved them to a closed dry ice storage container for 48 hours (to replicate how our viruses...from the dry ice). After 48 hours of CO2/dry ice storage, we then thawed the samples at room temperature...thawed, there was no effect on the pH of the samples stored in our lentivirus tubes (Fig. 1). However, once...

  29. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...then inoculated for storage on Thursday. The lab prepares bacterial glycerol stocks on Fridays. From each...used for long term storage (one is kept at Addgene and another is sent to a storage facility). The third...courier and then the journey of transformation and storage begins. Some samples are submitted as bacterial...intact. Some deposits are sent from labs nearby in Boston and Cambridge. Many deposits arrive from labs around...sample can be tracked easily at each step in the storage process. After the original sample is barcoded,...entry into Addgene’s repository. For long-term storage in our facilities, every sample is transferred ... - DNA sent from depositors, bacterial glycerol stocks, prepped DNA ready for our quality control processes...

  30. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...support through the customer’s perspective is good. If you’ve never participated in a customer support conversation...the types of questions customers ask, and there are also differences in customers’ personalities and their...reviewing customer data sets, troubleshooting protocols, analyzing information, and solving customer problems... product issue in the lab. The issue was with a custom-made reagent to manipulate cells (manufactured ...multiple languages - that makes communication with customers from around the world easier. You should also ...expectations. If you are willing to listen to a customer’s concerns and are patient enough to find the real...are the most popular ways to communicate with customers. Online live chat is a convenient alternative ...

  31. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...amber (UAG) stop codon by glutamine insertion supF (tyrT)   Suppression of the amber (UAG) stop codon by...or protein expression. If you were to include customized strains, the number is probably in the thousands... your plasmid or carrying out your experiment. History of E. coli strains E. coli are gram-negative, rod... DH5alpha and DH10b (also known as TOP10). The history of B strain is a bit more convoluted due to researchers...researchers sharing and renaming samples throughout history. It was likely isolated in 1918 but was first referred...Streptomycin MC1061 derivative. General cloning and storage, blue/white screening, leucine auxotroph. F- endA1...mrr-hsdRMS-mcrBC) λ- DH5alpha   General cloning and storage of common plasmids, blue/white screening. F- endA1...

  32. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...grow this initial stock and create a set of vials of ‘Master’ stock, which can be stored in liquid nitrogen...some of these vials will be stored in a separate backup liquid nitrogen store to prevent loss from equipment...used to produce a set of ‘working’ stocks of cells. Working stocks should be frozen in multiple vials ...plenty of maintained freezer stocks. It is vital that the Master and Working stocks of cell lines have the ... monitor the stock levels you have in liquid nitrogen for your Working and Master stocks of each cell ...and money but cells lose provenance (this is the history of the cell line consisting of records of the origin...used up. This ensures you can retain the maximum stocks and use of the original Cell line you purchased...

  33. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...dot plot, looking at a histogram vs antibody plot would be better. The histogram shows peaks of high signal...gates, just based on a histogram plot instead of a dot plot. However, the histogram plots look a bit different...lower signal, or downregulation. Figure 3 shows a histogram for a yes-no dot plot, like the one shown in Figure... Figure 1.  Figure 3: An example of a histogram plot for a yes/no flow cytometry readout. Created...Created with biorender.com.   Using histogram plots to quantify smeary plots For smeary expressions, ...for a signal peak that shifts to the right on a histogram plot and lower for a signal peak that shifts to...for yes-no readouts.      Figure 4: A histogram plot for a smeary antibody readout. The orange...

  34. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...point.   What makes a story? A story is a narrative connecting cause and effect. Stories can and should be... or notes. Listen to their stories and narratives (see below “Tell a story”) and note how they build suspense...hear material delivered in the form of a story. Using stories is one of the best ways to make you and ... a lab meeting (maybe not there either). Think Keystone Keynote presentation or Ted Talk. You want to ...me and wished I had been more careful. 3. Tell a story We are biologically programmed to listen and remember...presentations engaging and memorable. If you read my story above about the presentation faux pas, you are more... striking images, creative ideas, and engaging stories as you encounter or experience them. Drop content...

  35. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...substrate (the histone-derived peptide) which can localise the construct specifically to the histone protein ...target the K9 (pcDNA3-K9 histone methylation reporter) and K27 (pcDNA3-K27 histone methylation reporter) ... remodeling. Chromatin is a complex of DNA and histone proteins that compacts cellular DNA into chromosomes...dangerous DNA damage. If the way DNA is wrapped around histones changes, then gene expression can change as well...The more frequently and tightly bound DNA is to histones, the more compacted it becomes. This prevents ...can occur by two main mechanisms, either through histone modifications, which can influence the transcription...plasmid-based biosensor to visualise changes in histone methylation in living cells. Essentially this reporter...

  36. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...clear that it’s also a story of community, cooperation, and sharing. Sharing this story is a truly special...celebrate twenty years at Addgene, we are sharing stories of our repository’s positive impact on science ...science and scientists around the globe. Our first story focuses on a technology that has changed virtually ...libraries, and they remain the most popular with our requestors. However, we can again see quick adaptation and...better. We therefore looked at depositors and requestors by country, and graphed the top ten countries...surprise that the USA is the largest depositor and requestor of CRISPR plasmids. But many other countries, ... to Australia, are significant depositors and requestors. CRISPR tools are developed and used all over...

  37. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...into small, well defined chunks, called stories, and work on stories during short periods of time called ...clarifications lead to the story being scrapped or broken up into smaller stories. Since this occurs before... sprints. The team is mindful of the number of stories we commit to for each sprint, to ensure we will...process for working on a new feature. We start with a story that describes the feature and what the criteria...accepting the feature as done correctly. These initial stories are usually a result of collaborations between ...stakeholders (other Addgenies). Once we begin work on a story, we continue to collaborate closely with each other...the new feature as done and we’re onto the next story. Figure 1: Addgene’s Agile Approach Sharing...

  38. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...for drafting my stories. In total, I prepare 5-6 stories for an interview, since one story usually has more...Instead, I preferred to prepare stories to answer interview questions. Story telling shares many of the same...of the theme of my interview stories as the characteristic which the story demonstrates I possess.   Bullet...helpful for my interviews in industry. Prepare stories to tell in your interview The STAR interview method...answer: a setting, a conflict, and a resolution. Stories usually have a theme too, which is something not...of questions. Writing a rough outline of these stories helped me remember them for the interview.  Prepare...problem, or making a key decision. Even deciding to stop pursuing a particular line of research is a deliverable...

  39. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ... researchers prefer to store freshly harvested virus at 4 °C instead of storing it briefly at -80 °C. ... in a given viral stock will give you a sense of the infectivity of your viral stock, save you time, and... compare it to some reliable controls. 2. Viral stocks may be sensitive to freeze thaw cycles. Depending...these can be increased by concentrating a viral stock. Different titering methods can reveal differences... your virus by ultracentrifugation of the viral stock, followed by resuspension of the collected particles...highly sensitive to freeze-thaw cycles and should be stored in single-use aliquots. Never use a polybrene solution...increase transduction efficiency by 1.5-fold (Stockschläder et al., 1999). 5. Check your packaging cells...

  40. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... them, and store all but one in liquid nitrogen. The frozen vials make up your master stock, while you...be used as your working stock. This way you can go through your working stock vial by vial before thawing...another vial of the master stock. This ensures that you have many cells stored in their original condition...Keep the hood clean Hoods should not be used as storage cabinets. Clutter not only makes it difficult to... make things more manageable, but it also keeps stock solutions from getting contaminated. Make sure cells...like their space. If they reach confluence, they stop growing (contact inhibition) and it takes them time...condition and can replace your working stock if it gets too old or contaminated. This also ensures that anyone...

  41. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...glycerol stock production and storage, we grow a little extra and transfer 100 µl of glycerol stock to each...that we are sequencing from the exact same stock that we store and distribute from. Of course, your culture...software such as Primer3 to help you design your custom primers. Remember, when designing a sequencing ... of our Sanger sequencing is done from glycerol stocks (overnight cultures with 15% glycerol), as opposed... culture. Sequencing directly from the glycerol stock saves us the step of purifying the DNA and gives...96-well PCR plates are prepared with the primer stocks for forward and reverse reactions, respectively...equipped to perform Sanger sequencing from glycerol stocks, so make sure to select the appropriate service...

  42. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...field in which covalent modifications to DNA or histones, the proteins that help package DNA, were associated...modifications, researchers used blunt tools like histone deacetylases, but targeted epigenetic modification...researchers additionally fused a TAL effector to LSD1 histone demethylase to demethylate enhancer regions (Mendenhall...regulate transcription, also includes light-regulated histone methyltransferases and deacetylases. CRISPR and... acetyltransferase increases levels of H3K27ac histone modification at specified loci. Charles Gersbach... Figure 1: dCas9-p300 adds H3K27ac marks to histones in promoter and enhancer regions. These marks ...and repair pathways work downstream of Tet1 to restore the DNA after cytosine removal. Recently, Albert...

  43. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...bound to modified proteins called histones. We can detect modified histones with “GPS for proteins,” ChIP-... of modified histones, a genomic region that has mono-methylated and acetylated histones, but not tri-...map with three coordinates: chromosome, start, and stop. For instance (chr3, 1, 10) indicates a stretch ...Locates ChIP-seq Proteins (for our example later, histones) ChiRP-seq RNA Hi-C DNA (genome-to-genome ...tri-methylated histones, functions as an enhancer. We can therefore denote enhancers as follows: (mono_methylation...of epigenomics: H3 – one of NANOG’s associated histone proteins K4 and K27 – locations of the amino ...usually housed in a single flask. Through a bit of stochastic magic and Poisson statistics, each sub-population...

  44. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... are provided in both open (no stop codon) and closed (including a stop codon) formats. The collection..., et al. Cell Rep. 2015. PubMed PMID: 26190106 PRESTO-TANGO: An open-source resource for drug discovery...receptors. Bryan Roth's lab has a newly available PRESTO-TANGO kit which allows researchers to interrogate...and Screening via Transcriptional Output-TANGO (PRESTO-TANGO) to screen the NCC-1 library of approved ...discovery. Researchers can use plasmids from the PRESTO-TANGO kit directly for drug screening or easily...Luckily, researchers from Douglas Densmore’s lab at Boston University have greatly simplified the process ...CIDAR MoClo Parts Kit are available as glycerol stocks in a single plate with every plasmid or as individual...

  45. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...thawing a fresh vial. In many labs, the history of the cells in storage may be a bit dubious due to lack of... especially when the lentiviral stock needs to be titrated. If storing for less than a day, lentivirus...For long-term storage, viral preps should be divided into single-use aliquots, and stored at -80°C. Some...want to consider concentrating your viral prep. Storage can also play a role in the success of your lentiviral...factors such as the transgene expressed, the viral storage buffers used, and other experimental parameters...dry ice/ethanol bath or liquid nitrogen prior to storing. Know your target cells Labs tend to follow the...serve as the cellular receptors for vesicular stomatitis virus.” Proceedings of the National Academy of...

  46. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...publishers. Nonetheless, the initial release caused a storm as the organization left open the question of how...for advice about the scope of the policy. End of story? Not quite. The PLOS policy on data access is a...statistical methods, omission of some data, etc.  The firestorm that followed implementation of the policy comprised...easily available, organized in a form that can be understood by others, balanced against how often such data...of research, raw data requires huge amounts of storage. For example, mouse behavioral geneticists may ...data should be included? We should recognize that storing data in a sharable form is indeed a significant...carried out. While no one is suggesting all data be stored in a retrievable format, we too rarely preserve...

  47. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...were discussed at the 2018 Keystone meeting for Chromatin. Note that histone genes frequently have many...context of chromatin?” Though it’s true that adding histones and chromatin architecture to every diagram portraying...Chromosome Architecture and Chromosome Organization Keystone Symposium. As it happens, there are a lot of scientists...transcription of a gene a megabase away, or looking at the histone methylation status at a double-strand break that...the presentations and posters I saw at the 2018 Keystone Chromosome Architecture and Chromosome Organization...Sharon Dent Archa Fox Elaine Fuchs Kai Ge Robert Kingston Thoru Pederson Phil Sharp Julie Ahringer Alexander...RANbodies) as versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl ...

  48. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ..., Word docs, and the like is likely stored in online file storage tools like Dropbox, Box, One Drive, ...commonly used online file storage tools, as illustrated in this video, and to data stored on institutional and...like image banks and sequencing files is likely stored in institutional and lab servers specifically designed... conveniently reference specific pieces of data stored externally in the record of research compiled in...fully supported API that enables development of custom integrations with other tools and resources. As... in the documents to data in the external file storage resources remain live. This means that someone ...research and commercialization community in greater Boston, while at the same time keeping one foot on the...

  49. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...CDS1, coding sequence with stop codon; CDSns, coding sequence with no stop codon; TAG, C-terminal tag;... own glycerol stocks for more frequent use. This way, if one of your new glycerol stocks gets contaminated...in your laboratory as plates of frozen glycerol stocks. It is very important that these stay frozen at...rounds of freezing and thawing will affect bacterial stock viability. When handling your MoClo plate, always...preferably on dry ice. Careful use of your glycerol stocks When your lab adopts the MoClo technology, you ...you will quickly go through your plasmid stocks. To avoid cross-contamination, we recommend retrieving plasmids... miniprep a lot of plasmids from your bacterial stocks beforehand. You can also use high-throughput methods...

  50. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...human cryostat tissue sections. J Histochem Cytochem : Off J Histochem Soc 38:923–926. https://doi.org/...Fighting with antibodies to produce immunohistochemistry images that are crisp, bright, and lacking in...avoiding background secondary binding Direct immunohistochemistry (Figure 2A)  How do you avoid having your...Randolph-Habecker J (2013). A Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free...Immunofluorescence, and Multiple Antibody Staining. J Histochem Cytochem 62:197–204. https://doi.org/10.1369/0022155413511620...Monoclonal Antibody to Mouse Tissue Sections. J Histochem Cytochem 46:977–983. https://doi.org/10.1177/002215549804600813...Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical technique for demonstration of specific binding...
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