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We narrowed to 29 results for: AdRed

Showing: 1 - 20 of 29 results

  1. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...conceptualize where the populations are, try drawing a quadrant over the plot that divides it into four, using...different populations by imagining (or drawing) a quadrant over the dot plot. Created with biorender.com....Biorender! While a helpful visualization tool, a quadrant will often be too clean for real-world data. Most...these populations, I suggest using the imaginary quadrant to help (Figure 5). Even though this real-world...real-world data is far messier than biorender plots, quadrants can still help me as a reader if I'm struggling...strategy from Barlow-Anacker et al., 2017, with quadrants to help visualize the different populations. Used... This flow data, from Boix et al., 2018, uses quadrants to gate and has numbers representing the percentage...

  2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... tested by Quadros et al. When delivering the ssDNA template and CRISPR machinery, Quadros et al. recommend...pups born, with typical efficiencies of 30-60%. Quadros et al. attribute much of this variation to differences... locus may also influence efficiency. However, Quadros et al. saw success with Easi-CRISPR across three...Channabasavaiah B. Gurumurthy, Masato Ohtsuka, and Rolen M. Quadros for assistance with editing this blog post. References...PMID: 26242611 PubMed Central  PMCID: PMC4525291 Quadros, Rolen M., et al. “Easi-CRISPR: a robust method...Central PMCID: PMC5434640 Miura, Hiromi, Rolen M. Quadros, Channabasavaiah B Gurumurthy, and Masato Ohtsuka...

  3. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...and spread over the second quadrant. The process continues until all quadrants are used, hopefully revealing...plate, first the bacteria are inoculated over a quadrant of an agar plate. Then, a stick is dragged through...revealing single, isolated colonies in the last quadrant after incubation. The challenge is usually successful...

  4. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...Dronpa that doesn't become inactivated as quickly. Padron is a monomeric, reversible PA-FP derived from mutating... / P141L / G155S / V157G / M159Y / F190S) (7). Padron’s “positive” switching behavior is the opposite ...opposite of Dronpa; when exposed to blue light, Padron is photoconverted from a dark state, to an activated state...bright green fluorescence. When exposed to UV light Padron is inactivated. The rs-Cherries (8) are another...UV-Violet Dark/Green N/A (high) High 503/ 518 Padron Monomer Blue Dark/ Green N/A (high) Low 503/ 518...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...experimentally-specific gates. Two-dimensional staining (quadrant gating) For our last example plot, I will show...cells (CD8+) from dendritic cells (CD11b+) using a quadrant gate. Within my “lymphocyte + single cell + live... two stains in relation to each other. Using a quadrant gate, I can divide my cell population into DN,...for each stain respectively). (B) Instead of a quadrant gate, I am free to use multiple individual gates...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...itself. Many thanks to our guest blogger Subhadra Jayaraman! Subhadra Jayaraman is currently a doctoral candidate...This post was contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University...

  7. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...determined it is most likely adrenal in origin. (Anatomy refresher: the adrenal gland is next to the kidney...

  9. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...the size limitations of ssODNs is Easi-CRISPR (Quadros et al., 2017). Easi-CRISPR in vitro transcribes...long ssDNA donors and CRISPR ribonucleoproteins. Quadros RM, et al. Genome Biol. 18(1); (2017). PubMed.  ...

  10. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    .... M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017). Programmable...., Waterbury, A. L., Koblan, L. W., Kim, Y. B., Badran, A. H., & Liu, D. R. (2017). Improved base excision...
Showing: 1 - 20 of 29 results