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We narrowed to 33 results for: Dre recombinase

Showing: 1 - 20 of 33 results

  1. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ... using multiple recombinases. To make multiple, precise genome edits, the recombinases used must have ...excision of the stop sequence. In contrast, Dre recombinase, the close relative of Cre, displayed an excision...determine the cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested...however, the other recombinase/reporter pairs were inactive, even at very high recombinase levels. These data...toxicity of these recombinases by crossing flies homozygous for a UAS-driven recombinase with flies heterozygous...tubulin-GAL4. For Dre, Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4...recombination in Drosophila, read on to learn about new recombinases suitable for this system. Gerald Rubin’s lab...

  2. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...inventory of recombinases and recombinase-dependent vectors. We recently released several recombinases in various...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...Calcium sensors and GABA sensors Optogenetics Recombinases And more! Control AAV For many AAV experiments...pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (124651-AAV9) Recombinase AAV Targeting and manipulating specific, genetically-defined...requested samples of AAV for testing purposes. To address this, we recently released our serotype testing...

  3. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...under the control of Flp recombinase together with inducible Cre recombinase (CreERT2), visualizes individual...Kranz, Andrea et al. 2010. “An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis...incredibly popular and powerful site specific recombinase (SSR) system, but it only gives you a single ...control. What is FLP FRT and how does it work? FLP recombinase, which is named after its ability to invert or...a similar fashion to the Cre system. The FLP recombinase recognizes FRT sites and initiates site directed...However FLPe is still less efficient than Cre recombinase. FLPe codon optimization resulted in FLPO, which...Cre (Kranz et al. 2010). These differences in recombinase efficiency are important to keep in mind when...

  4. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ... EGFP Recombinase AAV Finally, we are excited to add Flp-expression AAV to our recombinase AAV inventory...Flp-expressing AAV. See our recombinase AAV inventory here New recombinases Plasmid Serotype Name ...inventory. Flp is another useful recombinase that can be used in combination with Cre for intersectional... µL size serotype testing) pAAV-hSyn-EGFP Recombinases Plasmid Serotype Name 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo...FDA-approved drugs. See our inventory of DREADDs / Chemogenetics AAVs New DREADDs Plasmid Serotype Name 75033...Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also expanded our inventory...activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab are especially useful for...

  5. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...pAAV-hSyn-DIO-hM3D(Gq)-mCherry Bryan Roth   Recombinases Recombinase systems such as Cre-lox allow for more...types, recombinases allow you to further restrict gene expression to areas in which the recombinase and ...and construct are coexpressed. Three new recombinases from the Cepko Lab are now available as AAV1. CRE-...83896 AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 ...SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral...plasmid encoding a Cre dependent version of the DREADD hM3D is now also offered as AAV1 (along with AAV...AAV2, AAV5, AAV8, and AAV9). Once activated, this DREADD acts through the Gq pathway and promotes neuronal...

  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ... in recombinase-dependent sequences. Recombinase-dependent sequences may be subject to recombinase-independent...date, we have analyzed hundreds of viral vectors and estimate that recombinase-independent recombination...pre-recombined population that does not require the recombinase for transgene expression. Once packaged in AAV...pre-recombined DNA could lead to transgene expression in recombinase-negative animals, producing misleading results...transgene expression but limits the likelihood of recombinase-independent expression. Next-generation sequencing...

  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...site-specific serine recombinase. First, the prime editor installs the recombinase “landing sites,” then...then the recombinase handles the installation of the donor sequence. The latest work evolves and engineers...engineers Bxb1 recombinase variants to optimize PASSIGE efficiency (Pandey et al., 2024). The best-performing...editing installs two sequences containing attB/P recombinase landing sites on opposite DNA strands. When the...edited sequence with the attB/P landing site. A recombinase integrates a sequence from a donor plasmid containing... in mammalian cells via continuously evolved recombinases and prime editing. Nature Biomedical Engineering...low-abundance targets or natively isolated complexes. To address these limitations, the Funabiki Lab developed a...

  8. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...only become activated in the presence of Cre recombinase, which is limited to the hippocampus. Thus, only...titers for efficient retrograde transport. For recombinase-dependent vectors, this can cause challenges ...impact of this phenomenon is to use a retrograde recombinase-expressing vector (e.g. Cre or FLP) to transduce...projection pathway neurons and then inject a recombinase-dependent chemogenetic vector directly into the...the neck bone,” but with about a hundred billion neurons and six hundred trillion connections (synapses....

  9. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...viral vector is used to deliver DNA encoding Cre recombinase under the control of the synapsin promoter. It... cre-recombinase...running, and you can learn more about this useful, DREADD-containing AAV here. But the top requested AAV ...

  10. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ... PMID: 30678704. Sparse cell labeling using recombinase competition Article contributed by Aliyah Weinstein...Weinstein Sparse Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for...binds to calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin...-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to showing that this vector ...

  11. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...First, instead of using piggyBac™, you could use recombinase technology (Cre/loxP) to remove the donor cassette...[7], it is generally not as efficient as Cre recombinase. However, the use of negative selection largely...to DNA toxicity. More importantly, unlike Cre recombinase, the PBx is highly specific, and has not been... you will need to screen somewhere between one hundred and one thousand clones to find a cell line with...

  12. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...2003). This is achieved through site-specific recombinases such as Cre or Flp that induce DNA recombination... "Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents." Scientific reports...recombination. Purification and properties of the Cre recombinase protein." Journal of Biological Chemistry 259.3...26611576. PubMed Central PMCID: PMC4971574. Hooper, Andrew, and Jamie Maguire. "Characterization of a novel...

  13. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...Cre-based Brainbow, but is controlled by FLP/FRT recombinase. When placed under different promoters, Brainbow...143(1):134-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide...Drosophila melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature Methods...

  14. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...mCherry)-EGFP 50457 AAV2 pAAV-hSyn-DIO-EGFP Recombinases Plasmid Serotype Name 87306 AAVrg, AAV5... Email us or check our website to access them! DREADDs Plasmid Serotype Name  44361  AAV1 pAAV-hSyn-DIO-hM3D...

  16. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...open-reading frame (DIO). Upon coexpression with cre recombinase (either from a mouse line or viral co-injection...specificity dependent on the promoter of the cre recombinase while still taking advantage of the robust expression...things in science, optogenetics is best used to address a specific question. Optogenetics is a tool and...

  17. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...once injected, the DREADD will only be expressed in cells that express cre recombinase. Typically, this ...consists almost entirely of viruses expressing DREADDs from Bryan Roth’s lab at the University of North...floxed human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control of the ...further specifies neuron-specific expression of the DREADD (Kügler et al., 2003), which can then be exclusively...mice, rats, or non-human primates. To activate the DREADD, CNO is delivered either through drinking water...

  18. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ... of certain genes. A popular method uses Cre recombinase on transgenic flox animals to introduce modifications...very old branch of science, dating back several hundred years, with historical ties to psychology and philosophy...an introduction to behavioral neuroscience, and address experimental design considerations used in all ...enough to target larger areas, typically a few hundred nl. Alternatively, for larger brain areas, repeated...

  19. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...strategies that combine prime editing with a recombinase to install large sequences (up to 10 kb) into... in mammalian cells via continuously evolved recombinases and prime editing. Nature Biomedical Engineering...This recent contribution by the Adamson lab also addresses the problem of RNA stability. Using a CRISPRi ...

  20. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...AAV serotypes, and the ever-growing variety of recombinase systems. Picking Ligands Picking the right ligand...used in chemogenetics are DREADDs and PSAMs. Using DREADDs DREADDs DREADDS, or designer receptors exclusively... Other DREADDs, such as the KORD receptor, increase this threshold and silence neurons. DREADD-activated...function (Saloman, JL, et al., 2016).   DREADD ligands Several DREADDs, including hM3D(Gq), have been derived...cellular responses. The cellular response set off by a DREADD depends on what G-protein and, consequently, what...what pathway the receptor acts through. The DREADD hM3D(Gq), for example, lowers the threshold for neuronal...popular agonist for muscarinic receptor derived DREADDs has been clozapine-N-oxide (CNO). However, concerns...
Showing: 1 - 20 of 33 results