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We narrowed to 194 results for: LAS

Showing: 1 - 20 of 194 results
  1. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción...podríamos estar más emocionados! Después de recibir la traducción, Addgene contrató a una traductora científica...
  2. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...factors impacting prime editing and found the protein La (a small RNA-binding exonuclease protection factor...They developed a new prime editor that incorporates La’s N-terminal domain, PE7 (Figure 2).  PE7 shows enhanced...the improved efficiency is at least in part due to La binding and stabilizing the 3’ end of the pegRNA,...
  3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable repression and activation of ....org/10.1038/nmeth.2600 Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) .../10.1038/s41598-019-54179-x Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD (2014) A Protein-Tagging...
  4. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...by an optimized CRISPR/Cas system. Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park ...expression and fluorescence imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell. 2014 Oct 23;159...
  5. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...spent one day at Addgene last summer, through the La Vida Scholars summer program. I was unsure whether... Amy was, which was the impression I got from the La Vida Scholars tour. After some time had passed, I...
  6. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...RNA-binding exonuclease protection factor La. They found that La, which is ubiquitously expressed in eukaryotes...tail of pegRNAs (Yan et al., 2024). The team fused La to the C-terminal end of PEmax to produce PE7. PE7... ~ ~ ~ ~ ✖ Fuse RNA-binding protein La to the prime editor Improve RNA stability   ...
  7. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...Goldberg GW, Duportet X, Fischetti VA, Marraffini LA (2014) Exploiting CRISPR-Cas nucleases to produce...Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable repression and activation of ...04901-6 Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA (2013) RNA-guided editing of bacterial genomes using....1038/s41564-018-0327-z Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) ...
  8. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...bacteria with a pressure to keep your plasmid. Viva la (bacterial) resistance!  What are antibiotics? Antibiotics...
  9. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...This editor includes the fused RNA-binding protein La to protect the end of the pegRNA. Adding 3′ polyU... epegRNAs) can improve either endogenous or fused La binding (Yan et al., 2024). Figure 9: epegRNAs...
  10. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...–567 . https://doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead...doi.org/10.1038/nchembio.1753 Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD (2014) A Protein-Tagging...
  11. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...Here at Addgene, we often refer to plasmids as lab or experimental tools. They certainly are very handy... Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz, F. (2023). Plasmid classifications. Plasmid...
  12. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...work is held by The Museum of Jurassic Technology in LA, where it is currently on display.   Many universities...
Showing: 1 - 20 of 194 results