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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 41 results for: RPA;

Showing: 1 - 20 of 41 results

  1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...RNA samples are amplified by PCR instead of RPA. Unlike RPA, this technique uses Taq polymerase which is...isothermal recombinase polymerase amplification (RPA) using a commercially available amplification kit...regions of gene S and Orf1ab gene are amplified. RPA reactions also contain reverse transcriptase to produce...fluorescence upon cleavage. Upon amplification by the RPA primers, the Cas12a-crRNA binds to the complementary...nucleic acids, the target sequence is amplified by PCR/RPA using biotinylated primers. These are then immobilized...

  2. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...amplified using RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-RPA, respectively...respectively. RPA is coupled with T7 transcription to convert amplified DNA to RNA for subsequent detection by ...first amplified through isothermal amplification by RPA. When Cas12a-cRNA base pairs with the dsDNA of interest...

  3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...Recombinase Polymerase Amplification (RPA) or Reverse-transcription RPA (RT-RPA), respectively. Amplified nucleotides...

  5. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant pathways (Liu...grows at higher cell densities than most of its counterparts, yielding higher production power from a given...

  6. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do just that (Doench... Find CRISPRko (Brie), CRISPRi (Dolomiti), and CRISRPa (Caprano) libraries for the mouse genome! Other...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...myofibroblasts to cell death via down-regulation of TRPA1 channels. British Journal of Pharmacology, 178(15... antibodies for the specific detection of human TRPA1. Scientific Reports, 9(1), 18500. https://doi.org...

  8. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...creativity, cleverness, and crafting skills have surpassed your colleagues. Success is sweet – as sweet as...

  10. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...again reached new heights on the Addgene blog: we surpassed 60,000 views for the month of September! Historically...

  12. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...llamas, and alpacas (and their undomesticated counterparts) - not exactly species that jump to mind when...

  14. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...mammalian plasmids differ from their bacterial counterparts, including how replication occurs and whether...

  17. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...and its gRNAs are also larger than their Cpf1 counterparts. Table 2. Cas9 Multiplexing Options  Multiplexing...
Showing: 1 - 20 of 41 results