We narrowed to 32 results for: Sell

...with the vacuole or lysosome. Rosella fluorescent properties Rosella is a dual color-emission biosensor...color fluorescence Rosella emits based on its localization. Table 1: Summary of Rosella’s excitation and ...fluorescent properties of Rosella.   Table 2: Effect of Localization on Rosella’s fluorescence emission. ...Lysosome  ~4.8  Red    Using Rosella to study autophagy in yeast Rosella is targeted to the cytosol when...Rosella is a pH-sensitive fluorescent biosensor that was recently deposited with Addgene by Dr. Mark ...lysosome for degradation and recycling. The key to Rosella’s autophagy-sensing abilities is that its fluorescence...about prior methods for studying autophagy and how Rosella improves upon them. Methods for studying autophagy...

...everyone, particularly if you’re more interested in selling a product than communicating your ideas with people...with our general mission to share- as opposed to sell- science. 4. You can prepare answers beforehand....small quibble. If you are not too worried about selling yourself and really do just want to get information...

...products, so you have some of the best ideas on how to sell these products to other scientists. Tailoring my...introduction to the financial world. It teaches you about selling products which is an important part of the business...

...company’s FAQs? A lot of companies and organizations selling biological reagents have a ton of data on the catalog...their products from you, unless they’re knowingly selling a bad product (in which case, you should probably...

...minimize jargon. Equally importantly, you really are selling yourself. A sales pitch that requires reading two... foot in the door. Your ability to interview and sell a vision for your contribution to a Department's...

...ways but success has been observed with Phagemids (Selle et al., 2020, Citorik et al., 2015, Bikard et al...used successfully in mice for just such a purpose (Selle et al., 2020).  Targeting a subset of bacteria in... Sci Rep 6: . https://doi.org/10.1038/srep19452 Selle K, Fletcher JR, Tuson H, Schmitt DS, McMillan L,...

...neuroscience trade. There were, of course, many companies selling antibodies, microscopes, and dissection tools, ...

...i.e. primer3) or provided by the major companies selling PCR reagents. Nowadays commercial polymerases are...

...Chinese medicine. There were hundreds of stalls selling a myriad of items, only a small number of which...

...joint. Silly ideas (mining local salt deposits to sell at farmers markets), and serious ideas (how to stop...enjoys spending time with her husband and two Jack Russell Terriers. More SynBio Reading Read more Addgene...

...account aimed at octogenarians is going to be a hard sell!) Digital platforms often have demographic data ...

...you can identify them in yourself and learn how to sell them. Types of Conflict Before explaining my approach...

...varies for different enzymes. Many companies now sell fast digest enzymes that can digest large amounts...

...the last presentation. Yet, PFs need to tell and sell their stories to get jobs. PFs (and mentors) can...

...currently available from Addgene. Rosella autophagy biosensor Rosella is a dual-emission autophagy biosensor... and cytoplasmic Rosella. When fused with a mitochondria-targeting sequence, Rosella can be used to track...). In the absence of autophagy, the untargeted Rosella remains in the neutral pH cytoplasm where it emits...fluorescence. Following the induction of bulk autophagy, Rosella is targeted to the vacuole in yeast or the lysosome...targeted autophagy of mitochondria, or mitophagy. Rosella can also be fused to a protein of interest to track...the cytosol-targeted and mitochondria-targeted Rosella at Addgene! Keima autophagy biosensor Keima is ...autophagy bionsensor via the same mechanism as Rosella: GFP is quenched in acidic environments, while ...

...PMID: 8692687 Tracking autophagy in yeast with Rosella, a pH-sensitive fluorescent biosensor A pH-sensitive...Australian parrot, the dual colour-emission biosensor Rosella consists of DsRed connected to a pH-sensitive variant...7.0) but not at vacuolar pH (6.2). The colour of Rosella therefore varies with cellular location: it is ... #71245; mitochondria, Addgene catalog #71247) Rosella provides an easy way to follow uptake of cellular...

...2 nuclease, Cpf1 (CRISPR from Prevotella and Francisella), was annotated in several genomes and is classified...one to characterize the Cpf1 nucleases. Using Francisella Cpf1 (FnCpf1), they employed an E. coli plasmid...

...editing in mammalian cells since 1998. In that year, Russell and Hirata from the University of Washington reported...inactivation in human cancer.     References 1. Russell, David W., and Roll K. Hirata. "Human gene targeting...

...pricing information that is sent from a buyer to a seller. However, Addgene is a bit different from other...

...Additional Resources on the Addgene Blog Check out the Rosella Autophagy Sensor Read about Aptamer Fluorophores...