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We narrowed to 854 results for: TOR

Showing: 1 - 20 of 854 results

  1. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...This Depositor Week, we're highlighting eight amazing posts on research done in our depositor labs. Some...written by Addgenies and some are written by the depositors themselves. All of them have cool new tools, ...scientists to use. As always, a big thank you to our depositors for sharing your science with us! Corralling ...with Tim Miles: While not technically a research story, I couldn't resist adding in this excellent Q&A ...one of our favorite posts from 2022! Wow! Our depositors do some really incredible science - I hope you've...try in your own work!) If you've got a research story you want to share on our blog, please reach out ...

  2. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...fluorescence or voltage indicators.   Viral Vectors and Voltage Indicators As previously mentioned...Voltage indicators are voltmeters for cells! In this blog we will review how these indicators work, their...as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness of fluorescence...upstream component, the chemical factor, of a naturally occurring voltage indicator. To give an example, a genetically...specific cell types is where viral vectors become invaluable. Viral vectors are mechanisms by which genetic...Resources on the Addgene blog Viral Vectors 101: Chemogenetics Viral Vectors 101: Optogenetics Fluorescent...their use as biological tools. Why Use Voltage Indicators? Electrical signals are integral to many facets...

  3. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many...types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral vector components of...of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for all the...NY): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from:...adeno-associated virus (AAV). The components of these vectors are also split across several plasmids (often three...called helper plasmids. Below, we’ll breakdown the vector components first by virus and then by function....respectively, act as RNA polymerase II promoter and terminator regions and are slightly different in sequence...

  4. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...understand this regulatory network. The transcription atlas and library The transcription factor library ...Transcription factors (TFs) act as the gate keepers for gene expression, turning transcription on and...Computational analysis was used to infer differentiation trajectories and identify both up- and down-regulated pathways...deliver multiple TFs to each cell, allowing for combinatorial overexpression programs on cellular processes...Resources  References Joung J. et al., A transcription factor atlas of directed differentiation. Cell. 2023. ...

  5. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...about her mentoring style. Set goals with a mentor: The secret of accountability To make a mentoring relationship...posts in this Mentoring for Scientists series, you have: realized the value of having a mentor, developed...finding mentors and, perhaps, asked someone to support your career development as your mentor. How do ...with a mentor.The responsibility of arranging meetings and setting the direction in a mentoring relationship...An undergraduate can mentor a high school student, a graduate student can mentor an undergraduate, a postdoc...postdoc can mentor a graduate student – you get the idea. There are many advantages to being a Mentor: Learn...want more great advice on mentoring, click below to download the "Mentoring for Scientists" ebook, which...

  6. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...opportunities: Try virtual mentoring at Mentornet.net. This is an online database of mentors you can tap for email...someone to be your mentor Mentoring is a “loaded” word.  If you ask someone to be your mentor, it may sound..., get more advice on mentoring: What Makes a Good Mentor? Choosing a Good Mentor for Scientists  ... are potential mentors all around you. This third article in the Addgene Blog Mentoring series will cover...meet, the more potential mentors you will identify. Finding potential mentors is one of the many reasons...tips for finding mentors in this recent blog 5 Strategies for Finding Your Ideal Mentor. One of the best...researcher Connie Cepko to learn about her mentoring style. Mentors at home and away We should all have a ...

  7. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...lentiviral vectors, how they work, and why you might choose them over integrating lentiviral vectors.  Before...integrase-deficient lentiviral vectors, let’s briefly revisit the basic biology of lentiviral vectors (for a more detailed...lentiviral vectors that are competent for one round of infection (AKA transduction in the viral vector world...life cycle of a lentiviral vector with an integrase-deficient viral vector. Created with BioRender.com...lentiviral vectors? There are several reasons why you might choose an IDLV over a lentiviral vector: 1. No...include detecting vector particle components or performing quantitative PCR titration of vector DNA in samples...Lentiviral vectors have been a staple in molecular biology for over three decades. Widely used across...

  8. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101:...create far safer viral vectors that can be used in BSL-1 or BSL-2 labs. Viral vectors are viruses that have...increase the viral vector’s carrying capacity. In order to allow the viral vectors to reproduce when experimentally...expressed via cells cultured with the vectors. This allows the viral vectors to reproduce only in experimentally...of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook...viral particle is called a virion. Finally, viral vectors, which are used in the lab, are distinct from viruses...copies itself and “splits” into two cells. Viral vectors Viruses are quite appealing as molecular biology...

  9. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    Published
    Jan. 10, 2019, 2:38 p.m.
    ...search of phages for therapy Phage Directory started out as a directory of phage researchers and the phages...can be sent out to the directory earlier, but the researchers in the directory are volunteering their ...says. As the labs in the directory step forward to help, the Phage Directory team must decide which labs...Creating a repository of phage data and information Since the inception of Phage Directory ~1 year ago...). The pair quickly teamed up to create Phage Directory in a whirlwind three days in November 2017. ...find much-needed phages for patients.   A phage directory is crucial because it's hard to find the right...a last resort, so a request for phages to the directory does not typically come out until nearly the last...

  10. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ... Esq and Carl Paratore, MBA, CPA to the Addgene Board of Directors. Katz is the Director of Intellectual...School and Paratore is the General Auditor at Tufts Health Plan. The addition of Katz and Paratore onto the...Learn more about Carl Paratore, MBA, CPA Mr. Paratore serves as the General Auditor at Tufts Health Plan...presence in the biotech sector in both patent law and general life science regulatory law. My role at Suffolk...employment front. Paratore: I work at Tufts Health Plan and my role there is general auditor. I oversee a ...the Board of Directors brings complementary experience and insight to the now 10 member board at Addgene...that Addgene was looking for new board members. Paratore: Kristen Darby, who’s also a board member, reached...

  11. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...a good mentor? Where can I find a Mentor? What is this I hear about “group” or “peer” mentoring? How do... been organizing mentoring programs for scientists and doing training to help mentors and mentees have...to learn about her mentoring style. Top 7 questions scientists ask me about mentoring In this blog series... scientists ask me about mentoring. Here they are: What makes a good Mentor? How do I choose an Advisor... do I ask someone to be my Mentor? I have a Mentor – what do I do now? What are the benefits of taking...seeking out mentoring as an important aspect to their ongoing success. There were unfortunate stories from researchers...did not serve as good mentors, but many had examples of very productive mentoring relationships with advisors...

  12. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...2022 Blue Flame Depositors Hall of Fame  Thursday: 8 Great Depositor Research Stories   Friday: Specialized...Hello and welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social...we’re certainly going to try!      Here’s our Depositor Week schedule:  Monday: Thank you post (this is...Specialized Depositor Shout-Out  We'll also be doing giveaways on Twitter, so keep an eye out there for a ...cool Addgene swag.  And, of course, to all our depositors: thank you so much!!!  (A special shout-out to...

  13. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...tend to have a story structure: beginning, end; cause, effect; lesson, learning. Storytelling is a crucial...even tell short stories during weekly lab meetings. When scientists tell good stories, they engage their...appreciation for their research. Long story short, telling a good story is something that can be learned, ... your career. Story Telling at work Don’t believe me? Here’s an example of storytelling to answer an informational...conclusion. Advice for Telling a Good Story Start at the start Any good story needs a solid point to kick off...of versions of your story and find the one that fits your audience. Enjoy the story you’re telling There...collective endeavour. Your story should be too. Use drama Use any rhetorical technique you have in your...

  14. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ... for a curated list of coregulators). Coregulators can function as coactivators and corepressors depending...complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex is composed...feature afforded by coactivator complexes suggests that coactivators may be integrators of multiple cell ... W. (2002). Combinatorial control of gene expression by nuclear receptors and coregulators. Cell 108, ...tissue and organ level by nuclear receptors (NRs), their coregulators, and their physiological endocrine...Nuclear receptors and their ligands NRs represent the largest family of transcription factors found in...illnesses, and the metabolic syndrome. Coregulators Coregulators constitute a group of some 400 molecules...

  15. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ... as a shuttle vector? Are viral vectors shuttle vectors? The production of viral vectors typically involves...their vectors in fast-growing bacteria first. In fact, most yeast vectors and many mammalian vectors are...shuttle vectors are involved in the production of viral vectors, we consider the viral vectors themselves...between species. Figure 1: Shuttle vectors are vectors that can function in more than one host species...Not quite. Shuttle vectors have to address several big differences in the ways vectors are used across species...needed to retain the vector as cells divide. In transient transfection, the vector remains episomal, meaning...of the vector, the deficient cells are grown in media that supplies the key nutrient. The vector provides...

  16. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...Production at Addgene Viral Vectors 101: What is a Virus? Viral Vectors 101: Viral Vector Elements ...nucleic acid cargo of your choice. First, a viral vector is selected to perform the transduction with: either...desired DNA to be integrated can be cloned into the vector that you have selected. The nucleic acid will be...produced, and viral particles are packaged, when the vector is expressed. Once the design and construction ... it’s time to make virus! The constructed viral vector containing the cargo of interest is then typically...layers of risk management when working with viral vectors. Typically, one day after transfection into the...variety of broadly applicable and popular viral vectors. Check out our viral selections to see if we have...

  17. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can ...Three generations of adenoviral vectors: the first generation vectors are deficient in the E1 and E3 regions... second generation vectors are deficient in E1, E2, E3, and E4. Gutless Ad vectors (helper-dependent (...creating adenoviral vector constructs. It consists of 2 plasmids: a shuttle vector (in which the transgene...Adenovirus type 5. Three generations of adenoviral vectors The adenovirus virion is a non-enveloped particle...medicine (2017).   1st Gen AdVs are stripped of regulatory genes E1 and E3. Without these genes, AdVs cannot...lysate is your primary low titer rAdV-S, and can be stored at -80C for later use or used immediately for amplification...

  18. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...method for targeting receptor expression as a ligand and receptor pair. Targeting Receptor Expression Using... translation to human patients. Picking receptors The receptor you choose is largely dependent on what... designer receptors exclusively activated by designer drugs, are G protein-coupled receptors (GPCRs), ...derived from muscarinic receptors. The most popular agonist for muscarinic receptor derived DREADDs has been...pharmacologically selective actuator modules, were developed with the goal of creating receptors with less endogenous...on the Addgene blog New to viral vectors? Browse our Viral Vectors 101 blog posts Read blog posts about... relies on genetically introducing engineered receptors that are only activated by otherwise inert drugs...

  19. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...specifically recognized by another factor, the receptor. The receptor could be something like an antibody...for multiplexed imaging for advanced monitoring of different factors, in different or the same cellular ...wide-range of uses to monitor the fundamental cellular building blocks. Physiological factors Unlike the previously...engineered into viral vectors, so if you want to use them, you’ll need to use the vectors (or redesign the ...directly proportionate to the amount of a given factor within a biological system. Basically, biosensors...antibody, enzyme, DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte...analyte. This signal depends on the system and the receptor, but readouts could involve fluorescence, pH change...

  20. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...of the Fc receptors FcγRIIB1 and FcγRIIB, inhibit) specific immune responses. These receptors are specific...so IgG isotypes bind to Fcγ receptors; IgE isotypes bind to Fcε receptors; and so on and so forth. There...of different receptors, but their downstream effects can be sorted into three main effector functions.  ... about Fc effector functions and all that antibodies can do! For instance, some Fc receptors, once bound... RA, Towers TL, Presta LG, Ravetch JV. Inhibitory Fc receptors modulate in vivo cytotoxicity against tumor...antibody's binding affinity (Janda et al., 2016; Torres & Casadevall, 2008) but…does it really do anything... the innate immune response, by binding to Fc receptors on other immune cells. (psst! Need a quick review...
Showing: 1 - 20 of 854 results