...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE pAAV.hSyn-FLEX.iGABASnFR pAAV.hSyn-FLEX.iGABASnFR.F102G pAAV-Syn-Archon1-KGC-GFP-ER2...different iGABASnFR have been developed and are suited for different applications. The iGABASnFR GABA sensor...pAAV.hSyn-FLEX.iGABASnFR.F102G) is noticeably dimmer than iGABASnFR, and accumulates in the endoplasmic...sensor (pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant (pAAV.hSyn-FLEX.iGABASnFR.F102G...larger fluorescent responses to GABA. Find the iGABASnFR and its F102G variant in AAV1 at Addgene! Archon1...

...Biosensors Yulong Li New viral prep pGP-AAV-GFAP-iGABASnFR2-WPRE AAV1, AAV5 Biosensors GENIE Project New...prep with multiple serotypes pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE AAV1, AAV5 Biosensors GENIE Project... prep with multiple serotypes pGP-AAV-syn-iGABASnFR2-WPRE AAV1, AAV5 Biosensors GENIE Project New... prep with multiple serotypes pGP-AAV-syn-iGABASnFR2(no bind)-WPRE AAV1, AAV5 Biosensors GENIE Project... with multiple serotypes pGP-AAV-syn-flex-iGABASnFR2-WPRE AAV1, AAV5 Biosensors GENIE Project New...

...Maryland College Park, but she quickly realized it wasn’t the place for her. “I went to grad school with ...science, but didn’t know what she would do if she wasn’t at the bench.  “I was literally sitting in my office...current position at Fred Hutch because her skill set wasn’t matching up with the local job postings. “There...

...something about myself and my future career. If it wasn’t something I liked – well, I was used to experiments... take shape, was when I started volunteering. I wasn’t sure yet what a career in science outreach would...angry, disappointed graduate students because I wasn’t sure what I wanted to do. A few weeks into volunteering...

... While easiest from a production standpoint, it wasn’t ideal for our Lab Protocol videos. It’s really ...: I loved the end result, but it was clear this wasn’t going to work for our other videos. For starters...possible when showing lab methods. So, this just wasn’t going to work for the majority of our educational...

... of lab work lived up to reality, and, if it is wasn’t a good fit for me, then I didn’t need to explain... is with until just before the interview). This wasn’t exactly my idea of a dream job, but I knew that...academic jobs do exist, but working in industry just wasn't for me, and as has been highlighted recently it...

...Further examination of the mck-PGC-1a1 mice found it wasn’t that they were simply immune to stress altogether...the Karolinska Institutet, the discovery really wasn’t what they’d expected. "Our initial research hypothesis...

...leads, I probably should have been nervous), but I wasn’t. The presentation went along smoothly and was followed... into this incredible experience.  Of course, I wasn’t presenting to upper management and building out...

...luminescence to get around these issues, but this solution wasn’t practical due to the low intensity of luminescent...comparable fluorescent proteins. This weak emission hasn’t stopped some scientists from using chemiluminescent...

...Twitter handle, but I wasn’t using them to my professional advantage. It wasn’t until I wrote a blog post...

...postdoc, I realized that working in the wet lab wasn’t the right path for me. I began exploring alternative...trajectories within the industry, but the transition wasn’t easy - I had to reinvent myself and redefine the...

...reading other reviews because, as I mentioned, I wasn’t an expert in the field. To find reviews, I just...hours and experiments. A comment about citing: I wasn’t sure if I should cite reviews or the primary literature...

...was still laborious as epifluorescence microscopy wasn’t able to give a clear view of cellular movement ... protein when using 2-photon microscopy as this hasn't been well reported for many fluorescent proteins...

...truly examined what caused this glowing phenomenon wasn’t until much later when, in the 1960s, Osamu Shimomura...

...regulates them? You may be surprised to learn that it hasn’t even been 50 years since the creation of the United...

...the things that really made me feel welcomed. I wasn’t just an intern but I was an Addgenie. I thought...

...I wanted to be. But prior to graduate school, I wasn’t sure a master’s was a good decision. So how do ...

...single-domain antibody fragment was exciting, and it wasn’t long until the field was off and running with camelid-derived...

...having a good interview experience.” Although luck wasn’t the only factor in Caitlin landing her job. All...

...Biosensor Tim Brown New viral prep pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE AAV1, AAV5 Biosensor GENIE Project...