We narrowed to 41 results for: golden gate

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Aug. 27, 2015, 2:30 p.m.

Data-driven design of Golden Gate Assembly overhangs greatly increases the number of fragments that can be used in cloning.

Aug. 1, 2023, 1:15 p.m.

Learn a variety of techniques you can use increase the fidelity of multi-part Golden Gate Assembly reactions.

Oct. 11, 2018, 12:30 p.m.

Learn about the best tips and tricks for modular cloning (MoClo) of plasmids!

July 8, 2025, 1:15 p.m.

GreenGate is a cloning system for rapidly assembling plant transformation constructs and is based on the Golden Gate method.

Jan. 23, 2014, 3:23 p.m.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Sept. 10, 2020, 11:45 a.m.

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

June 12, 2014, 3:30 p.m.

Looking for a way to test a lot of different parts in your cloning, or maybe interrogate some complex genetic circuits? MoClo just might be the approach for you!

March 12, 2024, 1:15 p.m.

We describe the different modular cloning kits available for different applications and organisms to help you find the kit that's right for your experiment.

May 14, 2024, 1:15 p.m.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Aug. 21, 2018, 12:31 p.m.

In this Episode of the Addgene Podcast learn all about the Synthetic Biology research going on in Tom Ellis' lab at Imperial College London.

Nov. 29, 2017, 2 p.m.

Fragmid is a quick, easy way to design and create CRISPR plasmids. Read about the Doench lab's new modular cloning-based approach in this blog post!

Aug. 29, 2024, 1:15 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.

Learn about new tools in Addgene's collection, including new biosensors for neuroscience research, plasmids for a common lab enzyme, and more!

May 5, 2026, 1:15 p.m.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Sept. 6, 2017, 1:01 p.m.

A roundup of all the 2023 guest posts on Addgene's blog!

Dec. 5, 2023, 2:15 p.m.

Recent highlights from Addgene's collection include tools for epigenetic engineering, tracking cell-cell interactions, and a new auxin inducible degron system for C. elegans.

March 23, 2021, 1:15 p.m.

Find tips for transforming arabidopsis with your plasmid construct of interest.

Oct. 25, 2018, 1:23 p.m.

An all-in-one CRISPR/Cas9 Multiplex Genome Engineering System for transfecting and editing cell lines.

Nov. 12, 2014, 3:54 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Dec. 22, 2016, 3:03 p.m.