A Control for All Seasons
Type
Blog Post
Published
Oct. 5, 2023, 1:15 p.m.
...example, if you are studying the effect of a new tau protein kinase inhibitor on slowing diseases progression...alternative but well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples...look for the molecular weight shifts indicative of tau phosphorylation. By including the positive control...western against a protein standard (P) to check for tau molecular weight shifts.
A negative control ...protocol. In the experiment above, you could repeat the tau western blot several times with the same samples ...variability. If one were studying the effects of tau kinase inhibitors on mice, for example, it would ...