...Emission Depletion (STED), and Stochastic Optical Reconstruction Microscopy (STORM). These dyes have specific...Throughout their history, fluorescent dyes have enabled the visualization of both organic tissues and...than was absorbed. This phenomenon is known as the Stokes Shift, or the difference in wavelength between ...technologies in fluorescent dyes/ligands Throughout the history of cell biology, fluorescent labeling techniques...fluorescent proteinFluorescent Proteins 101: A History of Fluorescent Proteins  ...

...skeptical reader of historical fiction (I can personally attest to this!). Following history from the Ice Age...Wrong - and the New Research That's Rewriting the Story by Angela Saini "This book is a smart and carefully...Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction. Two dueling magicians in the...of England by Edward Rutherfurd "A 10,000 year history of southern England. While the length may be intimidating...

... examples with plasmids deposited at Addgene.  PRESTO-TANGO: The ligands that activate many GPCRs are...Roth’s lab created an open source resource called PRESTO-TANGO (parallel receptorome expression and screening...transcriptional output - TANGO) (Kroeze et al., 2015). PRESTO-TANGO uses a modified Tango beta-arrestin recruitment...ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab...McCorvy JD, Giguère PM, Sciaky N, Roth BL (2015) PRESTO-Tango as an open-source resource for interrogation...

...initial reversal drive cannot restore the exact original sequence, it can restore the original protein-coding...are eliminated by natural selection, gene drives distort inheritance in their favor and consequently can...they should not be relied on exclusively because historical studies of pathogen research have conclusively...using a recoding strategy; a subsequent drive can restore the wild-type sequence (save for the residual sgRNAs...

...Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is the shift in wavelength... most FPs, Stokes shift is less than 50nm (often much less).  For LSS proteins, the Stokes shift is ≥ ...

...when balancing a handful of demanding courses, a capstone paper I really wanted to hit out of the park, .... I need it just as much in my current role as Customer Support and Operations Manager at Addgene. In ...about to keep silent and move on to other things…. Stop! Take a step back and think of a time you’ve lead...collaborator or co-worker and showed that you really understood their side of an issue. Maybe you also showed...article from The Muse. Kai Strickland is a former Customer Support and Operations Manager at Addgene and ...

... wrong. It brought the whole field to a grinding stop and only now, 2 decades later, is it finally back...showing that AAV-mediated delivery of the RPE65 gene restores vision in the dog model of LCA (Lancelot, one ... 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector for ... again 3. Acland G. et al., 2001. Gene therapy restores vision in a canine model of childhood blindness...PubMed PMID: 11326284. 4. Bennett J, 2017. Taking Stock of Retinal Gene Therapy: Looking Back and Moving...

...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ... factor. Zeocin is handy because it can be a one-stop shop for selection across systems and species. The...entirely detoxified, even when Sh ble is expressed (Trastoy et al). Thus, it may not be a good choice for experiments...Hadley, W. K., & Brooks, G. F. (1986). Effect of storage temperature and pH on the stability of antimicrobial...11, 56. https://doi.org/10.1186/1475-2859-11-56 Trastoy, M. O., Defais, M., & Larminat, F. (2005). Resistance...

...celebration, we thought it only fitting to share some stories of ways we’ve turned the available into the accessible...countries, import and export regulations, differing customs processes, and the distances the packages needed...other things, could ensure our packages navigated customs and arrived at their destination in a timely manner... to Design a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture Collections...scientists, from techs to grad students to professors, stopping by to say, “Everything I know about virus, I learned...

...chose needed to fulfill certain requirements: Customizable to our specific needs Modern and well-supported...the order and usage of the scripts needs to be customized in the pipeline for the specific needs of the...Since each process can use a unique container, we customized each container for its particular purpose and...bbduk process.    At first, to make sure we understood the concept of channels and implemented the input...user interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment our ...