We narrowed to 884 results for: ica

...Diecke, S., et al., Recent technological updates and clinical applications of induced pluripotent stem...opened up vast possibilities for the clinical and therapeutic applications of iPSCs. The generation of human... patients, specifically children and patients with skin disorders for research and clinical trials, as...derivation of clinical grade hiPSCs, reprogramming methods that do not involve modification of the original... OriP and induces amplification of the episomes concurrently with DNA amplification in the host cell. ...system thus provides a practical way to generate transgene-free, virus-free clinical grade hiPSCs from easily...therapy trials and remains a safety concern in the clinical field [4]. Three major reprogramming methods are...

...experiments with too few replicates leading to suspect statistical significance (you can read more about...someone has replicated it precisely. You can find a great discussion of different types of replication in Nosek...formulating new biological concepts. However, rates of reproducibility for preclinical studies, as reported...perfect story for a “high impact” publication can lead to the publication of rare flukes showing positive...more practical) is pre-registration and review of experimental design as part of the publication process...on solutions to reproducibility issues in the biological sciences and featured speakers from academia,...Overview - Jeffrey S. Flier, Researcher at Harvard Medical School, former dean of the faculty of Medicine ...

...Series 4. Become a science communicator More and more fellowship applications are asking for a 200-300 ...good quality application. Even following this advice, only about 50% of my grant applications have been ...your grant application to your peers and collaborators can also help you get some critical insight into...do need to be able to research and write your application, then give it to someone else to read and / or...it). It’s easy for reviewers to spot when an application is rushed (whether the idea isn’t properly researched...researched / developed or the application is full of typos) and these are unlikely to get past the initial...someone else in the department) to read over your application and give you feedback. They’ve most likely been...

...production and purification that yield high titers (see also AAV protocols for production, purification and titration...higher specificity and reduced technical variability compared to chemical tracers. rAAV2-retro also has...ability to spread trans-synaptically on to subsequent neurons. AAV vectors are replication incompetent and cannot...intracerebral application, which is more invasive and has a much higher risk of complications. References...between different brain regions. For example, communication between the hippocampus and the prefrontal cortex...memory, a special type of memory which includes autobiographical events (see Jin & Maren, 2015). Directional...intracellularly spread away from the site of application and aid in visualization of the morphology of...

...research? I grew up knowing little to no biological or biomedical scientists and had no idea what the daily... global problems. Biomedical research, for instance, contributes heavily to medical practice, and understanding...comes your way. Think critically about whether it’s something you want to dedicate your time and energy...These conferences have a lot of programming specifically dedicated for career advancement, and for students...entire page dedicated to different career options from science policy to science communication and much ...the Daily Psychological Experiences of First-Generation College Students. Social Psychological and Personality...hazardous liquids. Working in a lab is nothing like a typical part-time or 9-to-5 job. I had to ask to find out...

...get there and more practically, when do you need to be there? A typical biomedical postdoc fellowship ... findings that fail to replicate. Plan experiments logically and strategically to increase your rate of...theJanelia Research Campus of the Howard Hughes Medical Institute. Today's postdoctoral fellows (PFs) face...need? Multiple first or corresponding author publications (published, not in preparation) A history of...submitted manuscript in a high-profile journal typically takes 6-12 months depending on the revisions and... to be ready to write by Year 3.5. Thus, it is critical to get an experimental system up and running very...The other items will follow. If you get more publications, you will be more competitive for fellowships...

...Waugh, 2002). The tag can also be proteolytically cleaved after purification, so as not to perturb protein...for downstream applications. Tag cleavage must be performed after primary purification and requires a ...thoughtful planning, affinity tags can make protein purification a cinch. Types of affinity tags, the proteins... Affinity tags are used primarily as protein purification tools (and can have secondary functions as detection...single protein is expressed, with the intent of purification, it will be expressed along with all other cellular...available antibodies, resins, beads, and other purification aids readily available. Choosing the best tag...tag to remain on your protein for downstream applications Below, we describe a few of the most common...

...the sandwich and typically takes less than an hour. However large proteins typically do not transfer as...validated applications, pay close attention to the controls listed on the vendor’s website. Typically, the ...downregulated in a system, detect post-translational modifications, quantify protein levels relative to standards... boiling in the presence of a reducing agent, typically containing thiols, to cleave disulfide bonds. ...gel on top of the resolving gel. A stacking gel typically has a different ionic strength and lower pH and...are “sticky” and have the potential to bind nonspecifically to the antibodies used for staining. To address...search, first pay attention to the validated applications listed on the vendor’s website and choose an...

...would be a good time to talk about the amplification and verification processes we use to ensure high-quality...your own experiments. Library amplification 101 Library amplification seems straightforward: E. coli ...represented. Typically, Addgene uses electrocompetent Stbl4 cells when performing amplifications, and we use...transformed with the library DNA, they grow and replicate, and then their DNA is harvested, much like one...library has its own depositor transformation and amplification protocol, but the overall workflow is similar...ll need to remember when undertaking library amplification and sequencing. Maintain representation of the...ensure a good transformation efficiency. For a typical library, Addgene usually does four to eight electroporation...

... expression for experimental, biotechnological, and medical applications. Researches can visualize proteins...of complex post-translational modifications. Extraction and purification of recombinant proteins from ...of proteins including antibodies and pharmaceuticals, specifically interleukins. Yeast expression systems...downstream applications. Cell-free expression systems however, are expensive and very technically challenging...modified via an array of post-translational modifications including protein folding, formation of disulfide...mRNA and the addition of post-translational modifications Learn more molecular biology in our Plasmids...expression systems used for protein production and purification. These include mammalian, insect, bacterial,...