PITChing MMEJ as an Alternative Route for Gene Editing
Type
Blog Post
Published
Feb. 23, 2016, 3:30 p.m.
...of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect repair” like...making it easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed ...systems
The ready-made PITCh plasmids available from Addgene are perfect for expressing GFP from a given promoter...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...26678082.
Find the plasmids from this publication at Addgene.
2. Dalvai, Mathieu, et al. (2015). “A Scalable...26456817.
Find plasmids from this publication at Addgene.
3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and ...57. PMID: 26701308.
Additional Resources on the Addgene Blog
Brush up on Homology Directed Repair and ...