We narrowed to 714 results for: MAK

.... Before you read out your plots, you’ll want to make sure you’ve also adjusted voltage and compensated...tips on adjusting voltage: During data acquisition, make sure that the signal for the positive population... dot plot in Figure 2. Quantification using MFI makes it easier to identify and study these subtle shifts...

...promise there is the ability share the information, making it available to everyone, that creates more breakthrough...looking for those “what could go wrong scenarios” and making sure we have adequate process and controls in place... in a particular area or profession, you need to make sure you have learned and mastered the fundamentals...

...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...however can interfere with other genetic pathways making it difficult to link a phenotype to the gene being...The increased efficiency of TurboID and miniTurbo makes PL a viable method for proteomics in model organisms...

...  The antibody-producing B cells that are used to make hybridomas come from animals that were immunized...ligand during your experiment, then you’d want to make sure that you choose an antibody that recognizes...disadvantages of monoclonal antibodies will help you make the best decision for your experimental needs.   ...

...is the fact that Cas9 can be easily programmed to make a DNA double strand break (DSB) in the genome wherever...cleaning enzymes (Aprataxin, Tdp2) that function to make ends better substrates for ligation. Although we...Cas9-generated breaks is useful if the intent is to make a knockout in your gene of interest, as it is prone...

...20,000 genes, it is not possible to test each one and make sure it’s working. You can only do that screen with...system by screening for genes that, when turned on, make melanoma cells resistant to treatment with a BRAF...please ensure that you are equipped and authorized to make and use lentivirus. Also, note that due to the size...

...Palladian Partners. “I was lucky to have been able to make that transition really smoothly,” says Susan. After... a very painful process. And certain stories can make you feel like ‘I’m a terrible writer. I have never...science communicators. “The power of a story is to make a human connection with a reader, whether it’s a...

...and share some thoughts from the community that makes us… us. “The Addgene not-for-profit is an amazing...resource. I really appreciate the work you do to make sharing reagents and protocols so easy!” — Nina ...people knew how to access Addgene, and that we were making it possible for scientists to both deposit and ...

...one light chain. When assembled, the four chains make a Y-shaped protein that can be divided into the ...use Fab fragments as primary antibodies, you can make your own antibody fragments as needed.  In contrast...format. Reporter-conjugated anti-IgG Fab fragments make good secondaries for cases where you need to pre-form...

...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...inhibition is spatially and temporally ubiquitous, making them a rather blunt instrument for controlling ...al, Nat Chem Biol 2018. PubMed PMID:29483642. Making protein-protein interaction studies easier with...

...scientists starting their own labs get excited about making their first deposit to Addgene. We are also ...cutting edge practices and benefits and these are making a difference. We serve scientists around the globe...about being able to do good in the world. We are making an impact.  {% video_player "embed_player" overrideable...

...end, you’ll have a better understanding of what makes polyclonal antibodies unique and what experiments...together, all the B cell clones in the immune system make different isotypes of antibodies that recognize ...other antibody formats. This low barrier to entry makes polyclonal antibodies a common reagent in many labs...

... always excited when depositors find new ways to make this workhorse protein even more useful! From FPs...retain normal neuronal function, this system may make optogenetics in specific cell populations even easier...applications, including optogenetics, and they should make it much easier to conduct functional studies in ...

...plasmid such as pX330. This cloning strategy not only makes it easy to create a single gRNA-expressing plasmid...clone up to 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages of Golden Gate...TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt...

...it also helps you to hone your writing skills and make a name for yourself. Pick a topic that is close ...’t you record a podcast or put some effort into making a video? Use your unique talent to communicate ...Since then, I have tried many things: photography, making videos, presenting in pubs and at science festivals...

...Requiring an additional plasmid for transfection can make these optimization experiments a bit more challenging...experimental volumes accordingly. While you may need to make some final adjustments, this is generally a good...additional resources. References Kumar M, Keller B, Makalou N, Sutton RE. Systematic determination of the packaging...

...vehicles for genetic information. Therefore they make excellent tools for genetic engineering. There are...vectors when used in experiments. Retroviral vectors make up a large toolbox used by researchers for (among...shRNA or miRNA. The ever expanding CRISPR field makes wide use of retroviral delivery systems, especially...

...spatiotemporal control or an “on/off switch.” This makes them great tools for providing a global overview... of the bait protein will be biotinylated. This makes it’s impossible to distinguish proteins that directly... proteins. This extended incubation period could make it difficult to detect proteins with short half-...

...proteins had to be re-engineered for each gene, making wide-scale gene activation seem next to impossible...activation compared to other CRISPR activators, making it a popular method for gene activation experiments...design (SunTag). This streamlines its delivery, making it a common choice for CRISPR activation. Newer...

...precipitates that permanently stain the membrane, making that section of the blot unusable for reprobing... if the mild is not effective. You may choose to make your own stripping solution using a recipe like ... Assess the stripping Now it’s time to check to make sure the antibodies from your first round of detection...

...biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation of errors ...ensure the entire team is using the same rubric and making the same decisions when analyzing the wide variety...the QC team leaves a note on the plasmid page to make requesting scientists aware of the identified discrepancy...

...crucial if we want to make sure that everyone can be informed and empowered to make their own decisions ...posts, lesson plans, or information briefs for policymakers. The rapidly changing landscape of genetics ...

...Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J,...plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, ...

...meeting in person is often much more productive and makes it easier to build relationships. Nonetheless, the...responsibilities Being a leader in an organization, you need to make sure the organization speaks in “one voice” whilst...such as leadership skills can only assist you and make you more qualified for future career opportunities...

... who keep changing their minds and have trouble making decisions. In my work as a thesis coach for graduate...be reviewed, bring them to the meeting. This will make it easy for your supervisor to support you. 3) Explain...articles on our blog Read Joanne Kamen's Post on What Makes a Good Mentor Resources on the Addgene Wesbite ...

...School of Medicine. In the Lindberg Lab we often make cell lines that overexpress genes of interest; more...Parents of infants will have no problem with this!) Make sure your colonies are well separated. For this,...taking both early- and late-arising clones, and making sure we always maintain a backup replicates of ...

...system), and the third post will describe how you can make your own mutant flies. Find Drosophila Resources...bristly hairs. Females do not have sex combs. To make genetic crosses properly, it is important to isolate...emerging at night. An incubator with a light cycle makes for very predictable work! When flies first emerge...

...the localization of your protein of interest is to make it detectable. Visualization can be achieved using... and their potential overlap, it is essential to make sure that the selected FPs do not influence each...your next localization experiment and invite you to make any fluorescent protein constructs you create in...

...usurped old methods or complemented them in ways that make them much easier. A special class of FPs, the FP...functionality with a single gene Although it is possible to make a timer system composed of two separate FPs (one... This one protein method simplifies cloning and makes your experimental setup less cumbersome. 3. Portability...

...that commercially available cloning kits can often make the process much more streamlined, because they ...the reaction in favor of the desired assembly to make the process close to 100% efficient. Gateway cloning...Multiple inserts: There are some cloning methods that make working with multiple inserts a breeze. Among them...