...stress and depression, and now researchers have reported a mechanism based on studies of transgenic mice... there is plenty more to explore. “It will be important to identify the key proteins and metabolites that...stress-induced depression. Agudelo LZ, Femenía T, Orhan F, Porsmyr-Palmertz M, Goiny M, Martinez-Redondo V, Correia...

...creating their own opportunities for career development and networking. Aside from the importance of the topics...transferable skills to enable pursuit of these diverse opportunities after training. One of the best recent discussions...

...mutations, both in mice and in human cells. But that important advance was quickly followed by even splashier...All this, and scientists are still working out important details in their understanding of how CRISPR/Cas9s...at the Broad Institute and MIT published another report showing Cas9's structural details. "Understanding...

...We continue to expand our educational content supporting the tools in our collection, such as blogs describing...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also...exactly what you need, faster. We’re also building a portal to easily share sequences and characterization ...

...I’m sitting in the San Francisco International Airport listening to the Lion King soundtrack and writing.... I love running and this event gives me the opportunity to dress up as Mr. Incredible, an added bonus...would like to give my thanks to all the Addgene supporters who spoke with me and deposited plasmids on this...

...focus on an aspect of communication especially important for a manager – getting feedback from the team... This can take practice and training. This is important because it creates a culture of engagement in ...  Seeking feedback frequently One of the most important paths of information flow is the one that brings...

...over $100 million in annual R&D expenditures to support increased public access to the results of federally-funded...on the global science enterprise lending their support to open scientific research data around the world...online survey of scientists showing overwhelming support for open access to peer-reviewed journal publications...

...sharing ideas for the company, but also talking about sports, movies, and just about anything. As our organization...unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style competitions...easy as possible. However, I think it’s just as important to take a break from work to share time with the...

...make a reporter construct. Fluorescent proteins (FPs) are also the foundation of multiple important technologies... itself is photoconvertible, new studies of spatiotemporal dynamics can be undertaken using available ...

...work was with TALENs, in collaboration with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began...correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the...transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via FACS, primer...

..., the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens...the Addgene family Perhaps the most heartfelt portion of the entire night was when Melina and Ken called... recognized. John and Stephanie Fan have been supporting Addgene from the very beginning and have played...

...had certainly realized the importance of seeking out mentoring as an important aspect to their ongoing success...takes us a decade or more for just the “training” portion of our careers. In that time we may have only 2...

...a scientist in training can adopt to ensure opportunities in the future. Studies show that the majority...ignore this in their cover letters. It is also important to express interest, even excitement, in a position... to highlight different skills and to get the important information in the right place on the page. 5)...

...contribution of students in their labs and the importance of the strong, cross-disciplinary collaboration...Prize. What did Dr. Doudna think was the most important part of their work? First, the discovery of a ... research in the scientific community and the importance of this type of experimentation to the eventual...

...Cepko 1. Work hard. There will be periods when opportunities arise. “Grab it!” 2. Don’t get caught up in ... to deadlines, writing up clear and attractive reports, being good at presentations and of course, networking...from heart and hustle. Each PI emphasizes the importance of passion, hard work, collaboration, and staying...

...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...cross-references with cell line catalogs and collections. Importantly, Cellosaurus also identifies problematic cell...

...degree is a means to gain employment, so it’s important to make sure a masters helps you get a job you...from the bench, look for “technical or product support specialist,” “field application scientist,” or ... career ladder is larger and there are more opportunities for promotion. It’s not uncommon for bachelor...

...components that make up a plasmid and why each is important. Our “Plasmids 101” series designed to educate...and selectively grown on antibiotic plates.  Importantly, because the bacteria from which plasmids are...expression plasmids, gene knock-down plasmids, reporter plasmids, viral plasmids, and genome engineering...

...worldwide and the presentation made it clear how important it is for us to improve our resources for this...Needless to say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles...microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists know about...

...therapy - avoiding the use of viruses is also important for social and regulatory reasons. 1.) The transposon...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows...

...” instead of levels because it emphasizes the importance of these very different roles. At any stage in...doing, managing or leading. All three roles are important to accomplishing any goal or finishing any project... or highlighting criticisms. To be given the opportunity to take on the role of leader, one needs to take...

...attendees an amazing opportunity to share their cutting edge science in increasingly important field.  Interested...through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the day. He was followed...

...salt causes arcing during electroporation which would cause the electroporation to fail). When incubating...limit (lower transformation efficiencies have been reported with longer incubation); however, you may need...

...purified protein involves immobilization on a solid support. This allows washing away of unbound material to...binding kinetics of the target ligand. Another important consideration is whether the affinity of an experimental...acids. Unfortunately, the location of biotin incorporation and to a lesser extent, the number of added ...

...with every possible option, we place the most important controls front and center, and make specialized... are in the pipeline. First, now that SnapGene supports “Collections”, which are shareable databases of... missing from our database, or if you see an opportunity to harness common feature detection in a new ...

...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached... as effective for other techniques, but it is important to be aware that the issue is not unique to IHC...

...included in them for downstream control – this is important later! Next, additional components are added -...times more flexible than the brain fibers and importantly distortion of the fibers didn’t affect their ...temperature proximal to the implanted fibers. As important as its capabilities are the practical considerations...

...(Addgene #104102 - #104105) using in utero electroporation or with AAVs (prepared with Addgene #104110...Figure 3) that can be introduced using in utero electroporation. Then at your preferred time points, sacrifice...with an index-optimized clearing agent." Cell reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke...

...new tools and protocols that they can easily incorporate into their own labs. This week we are pleased...following sucrose treatment. Moreover, several reports have shown that cells treated with sucrose or other...test this approach with different capsids. Most importantly, because our AAV yields are improved we will ...

... RNA templates to DNA but suffer from several important shortcomings including low processivity (ie. the...29109157. Nanobody toolkit to monitor retrograde transport at the cell surface Article contributed by Marcy...functionalized nanobodies to study retrograde transport from the cell surface. Their toolkit includes ...

...probes, such as antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs...of antibody-combining sites within the variable portions of heavy and light chains. Proceedings of the ... PMID:4560694. PubMed Central PMCID: PMC427010. Porter, R. (1959). The hydrolysis of rabbit y-globulin...

...stylistic consistency. If you’ve ever doubted the importance of copy editors, the New Yorker’s “Comma Queen...review were discussed. Science communication opportunities If you are interested in the science communications...summer program that gives graduate students an opportunity to work in radio, print journalism, and other...

...sort of talent agency for scientists, where we support health science graduate students with innovative...fund that would apply a percentage of returns to support student projects, but it quickly became apparent...The composition of the management team is more important than the product itself -- investors would rather...

...said. “We know CREB is really important, but where is it really important and when? That is not known.”...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...

...facilitate a fast and agile MTA process. This is important to keep up with the number of requests and deposits...for both research and commercial purposes. It’s important to note that it’s the depositing institute that...signatures from two different institutes. Our online portal automates a lot of the process, from notifications...

...samples stored in our AAV tubes (Fig. 1). It’s important to note that the change in pH only occurred when...., 2014). Based on our results and the results reported elsewhere, we recommend that once you receive ...MC, Fitchmun MI. Protein instability following transport or storage on dry ice. Nat Methods. 2013 Apr;10...

...scientists. In addition to maintaining a laboratory to support both individual and community science projects,...experiments. For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept... involvement and provide all our members the opportunity to do molecular biology, we’ve developed community...

...when performing amplifications, and we use electroporation to ensure a good transformation efficiency....library, Addgene usually does four to eight electroporation reactions using a total of ~400ng of library...hope this post has helped clarify some of the important considerations when using, amplifying, and verifying...

...-hyg will be used to create gene deletions of important unknown regulatory regions in Pseudomonas and ... into a bacterial strain. The sensor will be transported inside a small 3D printed robot that will “swim...the user.   At Addgene we're happy to be able to support diverse groups of scientists working in a wide ...

...difficult during the pandemic era, with fewer opportunities for spontaneous conversations. What we are missing... minds. The final screen can also be saved and exported as a picture. I believe that showing something...fades away the longer we are on it – so it is important to take breaks. After 1 hour (or another designated...