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...call Epi-fluorescence microscopes. (See left image above for an example image). Point Scanning Confocal Microscopy... plane to reach the detector and all light from above or below is excluded. Parallelized Confocal Microscopy...due to limitations in light penetration mentioned above (5). Thick static samples Ex: Fixed tissue sections...sectioning. Point scanning confocal microscopes (see above) often provide the highest quality images for this...

...fluorescence microscope where the light source is mounted above (epi) the specimen and the excitation light passes...technique that allows imaging of living tissue up to about one millimeter in depth. Two low-energy photons ...response, but the study of these dynamics was still laborious as epifluorescence microscopy wasn’t able to give...skin. Two-photon intravital microscopy. The video above depicts the infiltration of neutrophils expressing...

...community.  You'll also find tons of information about Addgene's ready-to-use antibodies, viral vectors...and four! So you'll also find Addgenies out and about, attending sessions, visiting posters, and leading...Sunday, from 12 pm - 2 pm. If you want to learn about, and practice, principles of clear, accessible writing...

...scientists using AAV to share practical information about their experiences. It will contain all of the juicy...data to find patterns that we can share with you about promoters, serotypes, expression patterns, and more... more. If you have feedback or suggestions about the AAV Data Hub, we welcome you to share your thoughts...

...expedite the application process.  More information about export control licenses is available through the...Industry and Security. If you have any questions about this or other Addgene shipping and ordering processes...customer support team at [email protected].  Curious about how rabies plasmids are used in the lab? Check out...

...supporters of science communication. To help you think about ways to effectively communicate your science, we... of programs SITN runs and learn how they think about communicating science. Listen to the interview ...Interview with Science Rapper Tom McFadden Learn about SITN's work at DayCon 2015 5 Reasons to Use Reddit...

...multiplexing options, but also check out our blog post about multiplexing with Cpf1.   Figure 1: Multiplexing...assembly.  Unlike some of the plasmids described above, this vector does not contain Cas9, so you’ll need...similar to the Csy4 polycistronic system described above, except that they use an endogenous nuclease system...The Proper Cas9 Variant for Your Experiment Learn About Multiplexing with Cpf1 Additional Resources on ...

...researchers time by quickly relaying key points about an unfamiliar topic or lab process. Along with our...goals, or to answer any questions you might have about Addgene. The page is divided into three main sections...a variety of roles at Addgene.  You’ll hear all about their career paths, future goals, and get some useful...

... Cepko. Listen to the podcast below to hear all about the research being conducted in Professor Cepko'...and mentoring styles. If you'd like to learn more about Professor Cepko's recent work on developing GFP-...the time to talk to us and look forward to hear about more exciting discoveries from her lab. Special ...

... Addgene’s repository which have been requested about 1,500 times from labs in 45 countries*. While the...this article in the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve’s lab as an example...fascinating Addgene history that you might not have known about. Here's the schedule for the week: Monday: Fun ...

...to thinking about the downstream results of actions. I’m also pretty adept at thinking about risks and ...background were in demand. Q: Can you tell me a bit about your job currently? What is your day-to-day like...

...you’ll see a summary of the main topic and more about each of the topics in the dropdown menu. These pages...out the new author pages, where you can read more about the Addgenie behind the blog post and find more ..., and keep you up-to-date. Be the first to know about new articles on the Addgene blog by subscribing ...

... if we could equip plants with a way to warn us about concerning growing conditions? That was the goal...d see in a photo of a field of crops taken from above. A trained machine learning algorithm was able to...tissue. Those might be the most important to know about, anyway! Interested in developing these sentinel...

...Synthetic Biology Learn about the Addgene, Seeding Labs Plasmid Grant Read about Plasmid Grant Recipient...HEAD outreach program, and educating the public about dietary health. 40:17 - 44:14 : Microbiome research...

...need them the most. Check back here to learn more about future awardees and their inspiring research. Additional...the Superhero 5k Find New Cancer Resources Learn About Our Grants to Rare Disease Research Resources on...on Addgene.org Check out our Cancer Pages Learn about Global Plasmid Distribution ...

...challenges locally. We look forward to more collaborations with Seeding Labs and organizations like it...Additional Resources on the Addgene Blog Learn About Our Recent Visit to China Read Zafira Castano Corsino...Scientific Conferences Resources on Addgene.org Read about Global Plasmid Distribution Check out the List of...

...more scientists offering to share their stories – about their research, their job hunts, and their tips ... that are looking for more detailed information about this new technology. Our Plasmids 101 series continues...like to know what topics you want to learn more about. Our post on “Making Your Own Competent Cells” has...

...synthetic yeast chromosome. Listen to learn more about Dr. Ellis, synthetic biology, synthetic chromosomes...Additional Resources from the Addgene Blog Learn about the Synthetic Biology Education Nonprofit BioBuilder...BioBuilder Plasmids 101: Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with...

...s Fluorescent Proteins blog topic to learn more about FRET and other techniques using FPs! Otherwise, ...Addgene are shipped as bacterial stabs? You can learn about what format your material will come as (and more...biology tools in your research or not! And learn more about the variety of careers available to scientists on...

...Blog Learn about photoswitchable kinases Learn more about fluorescent biosensors Learn about using phosphoserine...

...Addgene helped unlock unprecedented scientific collaboration. Together, we distributed 184,824 materials ...help and guidance. Every plasmid shared, every collaboration formed, and every breakthrough enabled brings...accelerating the future of science. Want to learn more about our latest developments? Subscribe to the Addgene...

...study probably doesn’t say as much about open access as it does about the state of peer review. 8)     ...University Open Data Access (YODA) Project, “a collaborative endeavor guided by aspirations to promote open...

... development at the center of her lab (pictured above). DePace holds yearly planning meetings with each... their work rather than overwhelmed or confused about what’s coming next. Goal setting and career planning...and their next steps. Addgene would love to hear about the steps taken in your lab to promote personal ...

...to COVID-19 Learn more about the Ginkgo COVID-19 plasmid collection Read about plasmids in the COVID-19... are showing an unprecedented amount of support about allowing use of plasmids for research by anyone....

... tumor antigen. I asked Howarth to tell us more about SpyLigase, its development, and its potential uses...were no weak links in the chain.  Addgene: What about the Streptococcus pyogenes protein domain made it...this purpose? What led you to it? Howarth: Our collaborator Uli Schwarz-Linek at St. Andrews discovered ...

...ReFigure Analyzing replications as in this ReFigure about whether Natronobacterium gregoryi Argonaute has ...functionality Collecting preclinical and clinical data about a disease or therapeutic Side-by-side comparison...share your science at We Share Science Learn more about Data Sharing Resources at Addgene.org Deposit ...

..., see the table below and read on to learn more about GeoCas9.   SpCas9 GeoCas9 Size 1368 AA 1087...vivo. What aspect of GeoCas9 are you most excited about? Thermostability? Longevity in human plasma? Something... Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome Engineering   ...

...engineered Learn about the parts of the AAV transfer plasmid  Resources on Addgene.org Learn about AAV Find ...plasmid! Check out this blog post to learn more about the AAV transfer plasmid! References: Naso MF...

...substitutions don’t fare much better: they largely abolished cleavage by both Cas9 and Cas12 (Kim et al., 2020...deamination by a base editor. We at Addgene heard about RNA-DNA hybrid guides at the annual American Society...Society of Human Genetics meeting. It’s great to hear about the creative new ways these tools are being developed...

... in the hold status and reach out to depositors about them, but we all know how easy it is to miss an ...happy to distribute unpublished materials. What about preprints? Addgene supports preprints and encourages...appreciate your feedback. If you have questions about depositing, check out our website, Help Center, ...

... 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection Resources...levansucrase that converts sucrose into a toxic metabolite in gram-negative bacteria. Plating on sucrose...

...that their teams are working well together and collaborating effectively with other teams. While they sometimes... they are as engaged as possible. To learn more about being an effective manager, read the other posts...our podcast interview with Connie Cepko to learn about her management style. Leaders look to the future...

...molecular biology, yet scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation... sterile filter unless otherwise specified. The above table lists some antibiotics commonly found in the...or light – do not add them to media hotter than about 55oC and store plates/stocks wrapped in foil if ...

...most popular tweet from the conference is shown above. The blog post I mentioned, John Doench’s How to...forward. To do that, we need to learn much more about cellular DNA repair pathways. An emphasis on cellular...at the Keystone meeting had great things to say about CRISPOR, a tool to help you design, pick, and close...

...with users of the software. We welcome suggestions about common features from anyone who uses SnapGene or...Product Management at SnapGene. She is passionate about building software that people love to use, and likes...  Additional Resources on the Addgene Blog Read about our SnapGene powered plasmid maps Learn how we use...

...Additional resources on the Addgene blog Read about Brainbow Learn about using AAVs for neuronal tracing Visit...page Additional resources on Addgene.org Learn about Addgene's viral service See our fluorescent protein...

...materials as quickly as possible. (To learn more about why MTAs matter, check out another blog post: Why...provide specific examples if possible Tell them about the Golden Stopwatch! Any organization that deposits...California Institute of Technology Cold Spring Harbor Laboratory  Dana-Farber Cancer Institute  Fred Hutchinson...

...during development. Read this post to learn more about recombinase-based state machines and how they function...Albert Cheng and Mark Wanner from the Jackson Laboratory for Genomic Medicine described Casilio, a system...your target gene. Read this post to find out more about how the Casilio system works and how you can customize...

...impaired NgAgo activity, with three mismatches abolishing activity. One-guide faithful NgAgo guide loading... how it’s working in the comments section! Read About NgAgo User's First Impressions Here References ... Additional Resources on the Addgene Blog Read About Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants...

...eyes open for any new technologies that will bring about the next revolution in biological research. We’re...Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your next ...Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...