960 results

Getting start with mouse models? Here, we break down how to cross mice to create double knockout lines and Cre-lox lines.

Aug. 6, 2019, 12:55 p.m.

There are many ways to study Dopamine including in vivo microdialysis and genetically encoded biosensors. Read on to learn about two recently developed biosensors: dLight and GRABDA.

July 30, 2019, 12:59 p.m.

Transient plant leaf transformation can save you time... months in fact. Learn more about this method and get some tips and tricks for designing your transient plant leaf transformation experiment.

July 25, 2019, 12:03 p.m.

Crossing the blood brain barrier is difficult for many AAVs. But, PHP.B has impressive CNS transduction in some mice. Learn more about how PHP.B and about its strain specific performance.

July 23, 2019, 12:46 p.m.

Learn about the different open resources for C. elegans like OpenWorm, microPublication, and 3'UTRome, and find C. elegans tools at Addgene.

July 18, 2019, 12:55 p.m.

Zebrafish make a great model organism for studying neuroscience. Here, we describe how optogenetics can be used to study zebrafish behavior like swimming.

July 16, 2019, 12:59 p.m.

Learn about the common techniques to creating engineered mouse models at embryonic stages and the pros and cons of different genome editing techniques.

July 11, 2019, 1:26 p.m.

Learn ways you can bring your science to elementary and middle school by hosting events and giving talks geared towards elementary and middle school students.

July 9, 2019, 12:52 p.m.

Learn about CRISPR activation tools for neuroscience including a SPH transgenic mice, neuron-optimized viral vectors, and high-throughput screening.

July 2, 2019, 12:51 p.m.

There are many tools available for studying the microbiome. Learn about plasmid tools for engineering gut microbes, detection, and synthetic biology.

June 27, 2019, 12:25 p.m.

Learn about the different ways you can introduce DNA or RNA into cells using methods such as transformation, transduction, conjugation, and transfection.

June 25, 2019, 12:54 p.m.

Learn about recently available viral vectors and viral vectors coming soon from Addgene's viral service including our new trial size AAVs for serotype testing, jGCaMP7c variant in AAV1, Flp-expression AAV, and more.

June 18, 2019, 1:15 p.m.

Addgene has many videos to walk you through lab protocols. Find videos about AAV purification, AAV titration, and the basics working with tissue culture.

June 13, 2019, 12:19 p.m.

Learn about free tools for making scientific graphics: BioRender, Vectr, Inkscape, Google Drawing, Smart Servier Medical Art, and Google Image Search.

June 11, 2019, 1:16 p.m.

Learn about new plasmids including optogenetic control of synaptic transmission, acoustic reporter genes, miRNA reporter vectors, and CRISPR-Cas9 delivery for in vivo screening.

June 6, 2019, 12:41 p.m.

Learn about the fluorescent protein work being supported by the Michael Davidson and Rogers Tsien Commemorative Travel Awards.

June 4, 2019, 1:02 p.m.

Read on to learn more about this technique to isolate specific genomic regions using CRISPR.

May 30, 2019, 12:44 p.m.

Learn about the latest CRISPR plasmids deposited at Addgene such as an E. coli genome-wide CRISPR inhibition library, SECURE base editors, and Nme2Cas9.

May 28, 2019, 12:49 p.m.

What you get out of a conference depends on what you put into it before, during, and after the meeting. Read on to find tips for honing your elevator pitch, the elevator pitch, navigating talks, networking, and social media.

May 23, 2019, 12:26 p.m.

FlipGFP is a genetically encoded caspase reporter that contains a flipped beta strand of GFP. There is no fluorescence unless the caspase cuts the cleavage site between the strands.

May 21, 2019, 12:10 p.m.

Addgene is hosting #AddgeneCRISPRweek on Twitter. Check it out for anything from CRISPR basics to new CRISPR tools.

May 13, 2019, 12:01 p.m.

CellTag is a lentiviral based tagging strategy that combines scRNA-seq and lineage tracking at the single-cell level.

May 9, 2019, 1:15 p.m.

CTD2 FASMIC is collection of plasmids that can help scientists functionally characterize thousands of mutations associated with cancer.

May 7, 2019, 12:39 p.m.

Learn more about unique organisms that are being developed as model systems in this blog post.

May 2, 2019, 12:41 p.m.

mNeonGreen is a bright green fluorescent protein. Along with mWasabi and mTFP, it can be used for traditional imaging, FRET, and multicolor imaging.

April 25, 2019, 3:01 p.m.

Create a more sustainable lab by making small changes in your day-to-day or aim for long-term conservation goals. Find easy action items in the blog post.

April 22, 2019, 12:26 p.m.

CRISPR-Sirius is a new tool to visualize genomic loci using dCas9. It's brighter than its predecessor CRISPRainbow and unlike fluorescent in situ hybridization, can be used in live cells.

April 18, 2019, 12:53 p.m.

The Addictive Brain is a new platform to communicate science to broad audiences. Check out this blog post to learn more and for some science communication tips.

April 16, 2019, 1:05 p.m.

Model organisms are species that are widely studied because they are easy to maintain and modify in the lab. Common model organisms include the mouse, the fruit fly, yeast, zebrafish, and the worm.

April 11, 2019, 12:39 p.m.

Learn about some things to consider when designing your fusions - where to make the fusions, the size and shape of the protein, and linker size.

April 9, 2019, 1:13 p.m.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

April 4, 2019, 12:53 p.m.

Addgene's conference rooms are named after gene names. Learn more about these genes including sonic hedgehog, sleeping beauty, groucho, and cookie monster.

April 2, 2019, 1:44 p.m.

Learn about recently available viral vectors and viral vectors coming soon from Addgene's viral service including calcium sensors, GABA sensors, voltage sensors, and control AAV.

March 26, 2019, 1:40 p.m.

This blog series showcases some of the new CRISPR tools we have at Addgene. Read about CRISPR-Cas12b for human genome editing, plant base editors, string assembly gRNA cloning, lineage tracing tools, and more.

March 21, 2019, 12:47 p.m.

Learn all about new plasmids available at Addgene including an anti-CRISPR optogenetic tool, the fluorescent voltage indicator Archon1, photoswitchable proteins, and a simplified 2in1 cloning method.

March 19, 2019, 1:31 p.m.

Exosome-AAVSs, found in small membrane vesicles that are secreted from cells, can be purified and have some advantages over traditionally prepared AAVs.

March 14, 2019, 1:54 p.m.

The academic job interview chalk talk is essentially a million dollar sales pitch about your research vision. Learn how to define your research vision, organize your chalk talk, and practice efficiently before your next interview!

March 12, 2019, 1:37 p.m.

Find ideas to make your research more environmentally friendly. In this blog post, learn how to you can evaluate products to choose more sustainable ones, reuse plastic, and repurpose regulated medical waste.

March 7, 2019, 2:39 p.m.

Stochastic multicolor labeling is a popular technique to label individual cells with different colors and allows for lineage tracing of cell populations over time. Read this blog post to learn about the different systems for various organisms.

March 5, 2019, 1:08 p.m.

Learn about the Reproducibility for Everyone collaboration and how you can bring reproducibility workshops to your conference or university.

Feb. 28, 2019, 1:34 p.m.

Download Addgene's Science Career Guide and find some career tips from Addgenies.

Feb. 26, 2019, 1:40 p.m.

Targeted mutagenesis helps molecular biologists make changes to a DNA sequence at a precise location. Read on to learn more about EvolvR, a CRISPR-Cas9 based targeted mutagenesis method from the Dueber Lab.

Feb. 21, 2019, 1:15 p.m.

Addgene welcomes Karen Katz, Esq and Carl Paratore, MBA, CPA to their Board of Directors. Meet them and learn more in this Q&A.

Feb. 19, 2019, 1:36 p.m.

Thinking about ways to make your lab more environmentally friendly? In the first installment of Bringing Sustainable Practices to the Lab, learn about how to recycle styrofoam boxes, plastic packaging, and gloves.

Feb. 14, 2019, 2:35 p.m.

A new open source tool for CRISPR experiments, CrispyCrunch, has been developed by the Chan Zuckerberg Initiative to design batch gRNAs, templates, and sequencing primers for CRISPR + HDR experiments.

Feb. 7, 2019, 2:16 p.m.

Leaving the lab bench for a career outside of academic research can be a daunting task! In this blog post, we describe some ways to ease the transition from bench science to the office.

Feb. 5, 2019, 1:04 p.m.

The tumor microenvironment is the cellular milieu that surrounds a tumor and contains immune cells, blood vessels, cells, and signaling molecules. What goes on in the cancer microenvironment affects and is affected and can either promote or repress tumor growth.

Jan. 31, 2019, 1:32 p.m.

Aptamers are nucleic acid oligos that bind to small molecules to induce their fluorescence and can be used to study RNA localization and dynamics.

Jan. 29, 2019, 2:27 p.m.

Stochastic multicolor labeling is a powerful tool for discriminating between neurons. Learn about how a new system named Tetbow (Tetracycline transactivator Brainbow) can bring brighter multicolor labeling to neuronal tracing experiments.

Jan. 24, 2019, 2:24 p.m.

Fluorescent biosensors can be used to measuring changes in autophagic activity over time. Several autophagy biosensors, such as Rosella, Keima, and tandem fluorescent proteins are described.

Jan. 22, 2019, 2:41 p.m.