We narrowed to 610 results for: STO;

1. Addgene Begins Distribution of Recombinant Antibodies

   Type

   Blog Post

   Published

   March 28, 2022, 4:30 p.m.

   ...2019), validated in both western blot and immunohistochemistry. Additionally, many of the hybridomas from...

2. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

   Type

   Blog Post

   Published

   Sept. 14, 2021, 1:15 p.m.

   ...Test multiple colonies before saving a glycerol stock to ensure the colony you selected contains the full...

3. New and Upcoming Viral Vectors - Spring 2019

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   Blog Post

   Published

   March 26, 2019, 1:40 p.m.

   ...AAV1 at Addgene! Archon1, a voltage sensor AAV Historically, electrical activity in the brain was measured...

4. Fluorescent Biosensors for Measuring Autophagic Flux

   Type

   Blog Post

   Published

   Jan. 22, 2019, 2:41 p.m.

   ...of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting...

5. Multifunctional Neural Fibers: Modulation from Gut to Brain

   Type

   Blog Post

   Published

   Aug. 31, 2023, 1 p.m.

   ...flexible than the brain fibers and importantly distortion of the fibers didn’t affect their function (the...

6. Viral Vectors 101: What is a Virus (and a Viral Vector)?

   Type

   Blog Post

   Published

   Aug. 18, 2023, 4:15 p.m.

   ...Virus? In M. W. Taylor (Ed.), Viruses and Man: A History of Interactions (pp. 23–40). Springer International...

7. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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   Blog Post

   Published

   April 12, 2018, 1:02 p.m.

   ...Biological Resource Center (ABRC) is one of two global stock centers for Arabidopsis thaliana (Arabidopsis). ...

8. What Do I Do Now? Academic v. Non-Academic Career Decisions

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   Blog Post

   Published

   March 9, 2017, 3:40 p.m.

   ...exit survey data from postdoctoral programs in the Boston area that demonstrate that 85% of leaving postdocs...

9. New and Upcoming Viral Vectors - December 2019

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   Blog Post

   Published

   Dec. 19, 2019, 2:40 p.m.

   ...We now offer AAV9 for 2 more constructs from Christopher Harvey’s lab. These plasmids localize opsins ...

10. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    Published

    July 7, 2015, 3:36 p.m.

    ...directed repair (HDR), which enables insertion of custom genomic modifications, is very robust in C. elegans...

11. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type

    Blog Post

    Published

    April 7, 2015, 4:21 p.m.

    ...that lacks endonuclease activity coupled with a customizable single guide RNA (sgRNA) that bears complementarity...

12. Advice for Moving Into Sales After Your Science Postdoc

    Type

    Blog Post

    Published

    Aug. 21, 2014, 3:15 p.m.

    ... and allows you to work with and alongside the customer. From there a move with either promotions or further...

13. Mentoring for Scientists: I Have a Mentor, What Now?

    Type

    Blog Post

    Published

    March 6, 2014, 7:12 p.m.

    ...track progress, adjust expectations, and record milestones in reaching goals. The more time and thought ...

14. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type

    Blog Post

    Published

    March 4, 2014, 4:49 p.m.

    ...who may not be experts, can you give me a brief history of optogenetics? How long has this idea been around...

15. Networking Follow-up: I Met a Person. Now What?

    Type

    Blog Post

    Published

    Jan. 12, 2021, 2:15 p.m.

    ... honestly, she was the only person who really understood what I was going through. (Parenthetically, when...

16. Reagent Repositories Are Speeding up Science During the Pandemic

    Type

    Blog Post

    Published

    Aug. 4, 2020, 1:15 p.m.

    ...support to maintain repositories’ capacity for storing, validating, and distributing such reagents are...

17. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type

    Blog Post

    Published

    July 21, 2020, 1:15 p.m.

    ...protocols and tips Find the best way to elute and store your plasmid DNA Learn about the different grades...

18. DAP arrays for multiplex gene editing

    Type

    Blog Post

    Published

    July 7, 2022, 1:15 p.m.

    ...editing at each site. A 75-nt human cysteine tRNA stood out from their initial testing. It outperformed ...

19. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type

    Blog Post

    Published

    June 1, 2021, 1:15 p.m.

    ...easily started and easily credited. And nothing stops these one-time favors from becoming starting points...

20. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type

    Blog Post

    Published

    April 28, 2021, 1:15 p.m.

    ... saved to your Mendeley library. The library is stored in the cloud, so you can access it from anywhere...

21. New and Upcoming Viral Vectors - September 2019

    Type

    Blog Post

    Published

    Sept. 12, 2019, 1 p.m.

    ...recently packaged a soma-targeted opsin from Christopher Harvey’s lab. According to their publication ...

22. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type

    Blog Post

    Published

    Nov. 20, 2018, 8:17 p.m.

    ...persistent and exist online indefinitely. PIDs historically referred to objects that exist online, but this...

23. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type

    Blog Post

    Published

    Feb. 13, 2024, 2:15 p.m.

    ...their potential binding affinity to major histocompatibility complexes (MHC class I and II).  There are...

24. Trading Spaces - Visiting Scientist Edition

    Type

    Blog Post

    Published

    Aug. 22, 2023, 1 p.m.

    ...institute may have a piece of equipment that is custom-built, hard to find, or inaccessible to you for...

25. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type

    Blog Post

    Published

    March 30, 2023, 1:15 p.m.

    ... system. TF screens can be designed to identify custom TF programs to acutely initiate disease onset, ...

26. Learning Technical Skills at a Lab Central Workshop

    Type

    Blog Post

    Published

    Feb. 20, 2018, 2:30 p.m.

    ...make lab processes more efficient. Being in the Boston area, we get many opportunities to learn from other...

27. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    Published

    March 24, 2016, 2:30 p.m.

    ...publication:         References 1. Richardson, Christopher D., et al. "Enhancing homology-directed genome...

28. Components of CRISPR/Cas9

    Type

    Blog Post

    Published

    Feb. 2, 2016, 5 p.m.

    ... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...

29. Advice for Scientists Starting a Lab

    Type

    Blog Post

    Published

    April 29, 2014, 1:43 p.m.

    ...tips for starting a lab. Or share your success stories below. And don't forget to stay tuned for the last...

30. Finding and Joining Your Dream Lab

    Type

    Blog Post

    Published

    April 8, 2014, 2:50 p.m.

    ... hires are simpler because of their documented history in science. Potential graduate students typically...

31. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    Published

    Feb. 2, 2021, 2:15 p.m.

    ...Consequently it’s best to draft a cover letter that is customized for each internship. Lastly, have your resume...

32. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    Published

    May 26, 2020, 1:15 p.m.

    ...mutate in the genome) and an out-of-frame H2B (histone)-GFP reporter into the genome of mammalian cells...

33. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type

    Blog Post

    Published

    March 17, 2020, 1:32 p.m.

    ...be expressed until the base editor corrects early stop codons within T7 RNAP. A phage encoding the deaminase...

34. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...interact with personnel from the industry and brainstorm with each other. The event also featured a seminar...

35. Antibodies 101: ChIP

    Type

    Blog Post

    Published

    March 10, 2022, 3:25 p.m.

    ...antibody on crosslinked proteins. In the case of histone modifying proteins, which bind quite strongly to...

36. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ...antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western...

37. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9...

38. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ...podcast segment As the primary cause for both the stomach flu and food-borne illnesses, noroviruses can infect...

39. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ... S., Lee, W., Walsh, N., Iozza, K., Huang, N., Preston, G., Drouin, L. M., Jia, N., Deng, J., Hebben, ...

40. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    Published

    April 27, 2023, 1:15 p.m.

    ...mechanisms by which retrograde AAV work are not yet understood, one thing is clear: this application opens a...

41. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    Published

    April 13, 2023, 1:15 p.m.

    ...in your gene of interest, for example. Some customization is required, but many viral constructs have ...

42. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    Published

    March 21, 2023, 1:15 p.m.

    ...associations: common colds, COVID-19, norovirus (the stomach flu), and many more. Their infectious nature allows...

43. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type

    Blog Post

    Published

    July 28, 2017, 1:30 p.m.

    ...transgene. One such marker is the white gene, which restores eye color to fly lines where the endogenous white...

44. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    Published

    July 6, 2017, 1:59 p.m.

    ...deposited at Addgene was used to produce the glioblastoma model for this study (Ref 6)). In addition, ...

45. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    Published

    May 25, 2017, 2:30 p.m.

    ...fluorescent proteins. This weak emission hasn’t stopped some scientists from using chemiluminescent proteins...

46. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    Published

    May 11, 2017, 2:30 p.m.

    ...one assay per gene approach that has been the historical standard. Specifically, in our recent publications...

47. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-M. A Digital PCR-Based Method for Efficient...

48. Addgene’s Viral Service - Why Virus? Why Now?

    Type

    Blog Post

    Published

    March 23, 2017, 2:30 p.m.

    ...people and organizations who helped us reach this milestone. Viral service supporters We would like to thank...

49. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    Published

    June 30, 2016, 2:30 p.m.

    ...these strains should not be used for long term storage. Additional Resources Review of restriction modification...

50. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...promote replication.  Selection markers for yeast Historically, scientists have utilized auxotrophic selection...