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Showing: 451 - 500 of 728 results

  1. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Alan Wong. The complexity of biological systems can hinder...iterative one-pot reactions with straightforward restriction digestion and ligation steps to build barcoded...

  3. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    .... These plasmids have reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other... after reverse transcription (RT-LAMP) and a colorimetric readout that can be completed in just 45 minutes...

  4. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...laboratory of Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will... function in receiver cells. There are over 40 trillion cells that make up the human body and G-baToN ...

  5. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...photostable YFP to date. As such, mGold is expected to contribute to experiments that require high photostability...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth...

  6. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...green, blue, red, and far-red. There is also a ratiometric GECO sensor (GEX), where one wavelength changes... working with a stably integrated biosensor. Ratiometric measurements with certain single FPs (such as...

  7. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...References and resources References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application...(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary...

  8. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...efficiency and any unpaired fluorescent proteins can contribute additional noise to the measurement. If the distance... FRET experiments are expected to continue to contribute to our understanding of diseases and even assist...

  10. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ... to prep large quantities of DNA because some countries just can’t receive bacteria through the mail due..., if you have any great lab automation tips and tricks, be sure to share them in the comments section....

  11. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...although both are of course preferred! When trying to strike that balance, you may wonder: how close is close...significant amount of flexibility compared to a strict single-compatibility sequence like NGG. Nonetheless...

  12. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...plays an important role in RNA processing and contributes to variability in RNA half-life, and ultimately... Rho-independent termination is also known as intrinsic termination, and it relies on the formation of...

  13. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...Cardiovascular and diabetes mortality determined by nutrition during parents' and grandparents' slow growth ...

  14. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...This post was contributed by Brittany L. Uhlorn, a PhD Candidate at the University of Arizona. Perhaps... next poster session! There many other tips and tricks for presenting at scientific conferences, so let...

  16. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...Updated Apr 6, 2021. This post was contributed by Laura Desrochers, a postdoctoral scientist at AstraZeneca... academic lab is closely involved with clinical trials or has ties to industry, the drug discovery process...

  18. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...standard curves, and normal controls must be run in triplicate.  The nice thing about qPCR is that since it ... of reactions, it does not need to be run in triplicates. ddPCR is an extremely accurate application, ...

  19. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...This post was contributed by guest blogger Iris Lindberg, Professor at the University of Maryland School...Cloning cells using an agarose overlay does have one tricky step. It is necessary to pour the agarose onto ...

  20. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...This post was contributed by guest bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss...relative nuclease efficiencies as well as to more stringent targeting rules that result in a decrease in available...

  21. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...genes for drug discovery or to understand the intricacies of molecular pathways. These screens have gotten...solutions have been found to get around this restriction. The most obvious workaround is to use a multi-plasmid...

  22. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...under which the plasmid is distributed.  All Addgene plasmids are distributed under the terms of the UBMTA...

  23. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...are treated with a drug of interest and gRNA distribution is analyzed in the drug-resistant population...6 gRNAs per target gene, and maintaining the distribution of each gRNA within the population is key. Loss...

  24. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to get around...

  25. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...multicistronic vectors work? Scientists have "borrowed" some tricks discovered in positive single-stranded RNA viruses...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...

  26. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how... short as 100ms (EBFP) or as long as 1 hour (mAmetrine1.2). However, for most FPs it is a few seconds ...

  27. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...This post was contributed by Laura Lee, a graduate student at Stanford University. Arabidopsis is a fantastic... it pretty much is, but there are some tips and tricks you can use to obtain optimum transformation efficiency...

  28. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...noticing unusual cases of acute hepatitis in the pediatric population. Once ~400 cases had amassed in places...removal of certain adenoviral genes that apparently contribute to virulence (Tollefson et al., 1996) – cell ...

  29. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...’ve got you covered in this blog, with tips and tricks for harnessing the homology-directed repair pathway...are overly confluent or don’t have sufficient nutrients in their media, they will not undergo DNA replication...

  30. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...conditions commonly encountered in human gene therapy trials. Human Gene Therapy Methods, 26(2), 71–76. https...Qu, G. (2005). Identification of factors that contribute to recombinant AAV2 particle aggregation and ...

  32. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...functions differently. Since Cpf1 loci are widely distributed across bacterial species, Zetsche et al. hypothesized...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...

  33. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ... This post was contributed by Jo Miller, founder and principal of J. Miller & Associates, Inc., a national...professionals is learning to succeed in grants through trial and error, through winning and losing funding, and...

  34. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...them! Note: A. Max Juchheim, Marcy Patrick, and Meghan Rego contributed to the writing of this article. ...

  35. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...an elderly family member, want to train for a triathlon, finally buy that charming fixer-upper house you... every school performance, chaperone every field trip or cheer at every soccer game. Tip: take turns going...

  36. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...changes to a gene sequence can cause unexpected detrimental results to the protein. Codon optimization could...ZE, Kimchi-Sarfaty C (2011) Understanding the contribution of synonymous mutations to human disease. Nat...

  37. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with... 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed plants." Plant cell...

  39. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the overarching knowledge gap. Aims...

  40. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/11...innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/07...

  41. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...than any other one. Luckily, you can do a lot to contribute to the relationship dynamic and set yourself ...notes on general protocol steps and other tips and tricks your mentor shares. Additionally, go ahead and ...

  42. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ... libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled libraries...cells at a concentrated seeding density while centrifuging. Spinfection transduction efficiency can vary...

  44. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...start answering questions. r/Science has pretty strict rules about not being too sales-y in posts which...term “implicit bias” which is apparently a troll trigger phrase these days). Redditors ask serious questions...

  45. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...vial of the desired cell line from a reputable distributor such as the American Type Culture Collection ...cloudy. Mycoplasma compete with host cells for nutrients and can alter expression of receptors, ion channels...

  46. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...doing so, they identified the nucleotides that contribute to the instability of Spinach, and were able ...superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat–containing RNA." Nature methods 10.12...

  47. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking Block is used to...validated antibodies? Many companies that produce and distribute antibodies validate them in a variety of applications...

  48. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...This post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College...Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were developed...

  50. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... and last updated Sep 24, 2020 This post was contributed by guest blogger, Addgene Advisory Board member...target sites occur ~1 in every 8 nucleotides, restricting gRNAs to 5 – 65% of the protein coding region...
Showing: 451 - 500 of 728 results