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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: ACE;

Showing: 181 - 200 of 610 results

  1. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...recombination; SSA, single strand annealing) took place and reconstituted the EGFP expression cassette. ...fragment containing the sgRNA target sequence can be placed in multi-cloning site (MCS) between EGFP fragments...extra precautions needed when dealing with RNA (e.g. face mask, and a dust free and specially cleaned work...mice that may have no mutation, costing money and space. As a rule, screen all of your pups unless you have...

  2. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...), the inner membrane space of mitochondria and chloroplasts, the extracellular milieu, and gram negative...should be taken into account when deciding where to place an FP within a fusion construct [1,2], i.e. positioning...mitochondria, lysosomes or they are secreted into the extracellular milieu. Many of the resident proteins within...

  3. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...is by no means exhaustive, but should be a good place to start when trying to pick your perfect promoter...promoter down regulation due to methylation or deacetylation. Ubc General expression mRNA Mammalian promoter...calmodulin. GAL1, 10 General expression mRNA Yeast adjacent, divergently transcribed promoters Inducible with...orientation to your gene Although this list is a great place to start, the tables above do not delve into the...

  4. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen...for DNA repair synthesis. The ssDNA strand then displaces one strand of the homologous DNA donor and can...formation of a DNA structure referred to as the displacement loop (D loop). The recombination intermediates...synthesized portion of the invasive strand is displaced from the template and returned to the processed...

  5. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...The source of those conflicts can frequently be traced back to one of a few trouble spots:  Communication...here is pretty intuitive: You need to be willing to face conflict head-on. That doesn’t mean you have to ...and compromising. Though there may be a time and place for avoiding, competing, or accommodating, if you...knowledge to resolve conflicts. Conflict in the workplace is inevitable, but having who can help resolve...

  6. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...consuming and difficult. Finding volunteers who had space to shoot was another hurdle. As we slowly started...reopened. But there were still many restrictions in place, most importantly, masks.  Besides the muffled voices...strict safety protocols to keep Addgenies safe. Space was at a premium, so I had to figure out a solution... we were finally refining the process down to a place I could be happy with it. Cycle 5 - The Future! ...

  7. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...precision editing of the dystrophin gene would take place, with minimal risk of off-target editing. In order...sites. Each paper characterizes additional, unique facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR...the desired phenotype. Precision genome editing faces the challenges above and more. One chief challenge...approval. Even with the challenges precision editing faces, it’s encouraging to see such progress in the more...

  8. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...finding yourself having issues, you’re in the right place! In this post, we’ll talk about western blot optimization... visible to the naked eye.   If you see a smiley face in your gel, or if you see smeared bands, your voltage...voltage is likely too high. If you’re seeing a smiley face at an appropriate voltage (usually 10–15 V/cm of...bleed!), cut off the upper left-hand corner, or place a double ladder on the lefthand side. Whatever you...dry the membrane, wet it with 20% methanol, and place over a light box to visualize proteins after transfer...

  9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... site and adjacent PAM. Your knock-in design may naturally remove the gRNA sequence or place it far from...100 bp homology on each side. For optimal repair, place the loxP sites precisely at the cut site for each...the oviductal lumen, tweezer-type electrodes are placed to mediate electroporation. Ohtsuka et al. compared...

  10. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...members of your field, and present your research. Face to face meetings can make favorable impressions on potential...Medical Institute. Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods...manuscripts. 4. Balance focus and exploration Researchers face a fundamental conflict. Science is about exploration...

  11. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...reaching the detector. The pinhole is specifically placed to only allow light from the focal plane to reach...coverslip at an angle such that when it reaches the interface between the glass coverslip and a sample in aqueous...within a few hundred nanometers of the glass/water interface. Super resolution microscopy Super Resolution ...sample thin (< 15 μm) or thick? These questions will place each sample into one of four categories that are...

  12. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...plasmids and your viral packaging service in the same place, making it easier to go from selection to viral ...to order at your convenience. After an order is placed, we’ll facilitate the necessary MTA, then start...your plasmid of interest in your desired serotype, place your order when ready, and we’ll take care of the...

  13. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...Can you imagine how much space a million plasmids would fill? Two weeks ago, Addgene edged passed our... hard to picture one million plasmids. How much space would one million bacterial stabs take up? If 81...upcoming months, we will be moving into a bigger space so that we can make an even bigger impact - we are...

  14. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...depends on a surface glycoprotein – the only viral protein present on the virion surface – making it critical... system in a novel way, by shielding host cell surface proteins. Researchers at the University of Virginia...

  15. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...factors – to specific sequences of DNA has become commonplace. This ability has opened up a world of possibilities...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...tips: First, she recommends using standard BsaI in place of the high fidelity version (despite what the original...

  16. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...transfers. When Addgene entered the plasmid sharing space, we heard over and over again that the legal documents...experiments with confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform... Recipients: Streamlined Processing: When you place an order, Addgene generates the MTA and emails it...

  17. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...relatively the same for quite a while and hadn’t kept pace with our growth over the last few years. Some of... professional, and modern. Instead of trying to place all of the content above the fold, we decluttered...elements and allowed our content to dictate the space. We wanted to comfortably tell our story, to get...

  18. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...perspectives. Such activities foster a mindset that embraces the unexpected and encourages exploration of tangents...ideas and approaches. Moreover, the ability to embrace serendipity fosters adaptability, a crucial skill...your academic journey, remember to leave a little space for the unexpected — it might just be the key to...

  19. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ... free online molecular biology tools all in one place. Plasmid mapping These tools are for viewing, editing... looking at plasmid maps and viewing sequencing traces, while the paid version provides more tools for...Removes non-DNA characters, such as numbers and blank spaces, from the text of a DNA sequence, making it ready...

  20. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ... a double-strand break, and when used with two adjacent gRNAs, can lower the probability of off-target... of the targeted region, whereas PAM-in designs place the PAMs closer together in the middle of the targeted...sites separated by 51-68 bp Use D10A for HDR Place intended insert in between nick sites Use 40 nt...
Showing: 181 - 200 of 610 results