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Showing: 181 - 200 of 520 results

  1. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...proteins reported by other research groups that performed BirA*-based affinity purification mass spec experiments...affect protein binding patterns. Hereditary Hyperferritinemia-Cataract Syndrome (HHCS), a disease characterized...

  2. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...overexpressed from a plasmid but overexpression may interfere with the system. Another method for studying protein... from them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized...

  3. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...identification of desired clones.   ...Becomes a powerful cloning tool Molecular biologists first saw the...applications. While these two strains ultimately perform the same function, there is some evidence that ...

  4. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...determines what secondary antibody you should use when performing indirect immunoassays. The Fab regions, on the...before applying them to samples, such as when performing IHC in a sample where your secondary antibody...

  5. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ... the Liu Lab found that mismatch repair (MMR) interfered with the desired resolution of prime editing ...enhanced prime editing systems improve gene editing performance at therapeutically relevant gene targets and ...

  8. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...similar rates to untreated controls. Ma et al. performed sequencing of 23 potential off-target editing ...advancement in our understanding of how CRISPR will perform in embryos but also shows that many more experiments...

  9. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...case, we naively assume that everything will go perfectly and our software simply follows a linear progression... need to be repeated. We also now have a good interface to begin tracking analytics data for lab procedures...

  10. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...concentration, and not all providers are equipped to perform Sanger sequencing from glycerol stocks, so make...Alternatively, you can use DNA-editing software to perform alignments of multiple sequences to visually check...

  11. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...abundance of a specific clone; lineage tracing is performed via barcode sequencing. Using the ClonTracer library...the time to talk with us and for providing this powerful resource to the Addgene community! Carrie Bhang...

  13. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...transcription factors found in metazoans. The superfamily is comprised of 48 human and 49 murine members...version of which will feature a simplified user interface, expanded numbers of curated molecules, including...

  14. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated gene silencing mechanism...be combined with high-throughput sequencing to perform multiplexed analysis of cells pre-treated with ...

  15. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...embryonic genes are individually depleted via RNA interference (RNAi). Scientists monitor the effects of RNAi...RNAi using four-dimensional contrast interference (DIC) microscopy and computer imaging processing. The...

  16. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...policy requires broad interest in issues at the interface of science and society, and an ability to talk...in the legal, ethical and policy issues at the interface of biology and society.     Additional resources...

  17. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...’t common to non-scientists and may make your perfectly reasonable paragraph unintelligible to a lay audience...Science Communication Skills 5. Practice makes perfect, apply for small grants too There are actually ...

  18. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...general questions about job responsibilities, performance evaluations, professional development, and company... research is a deliverable. Find 10 steps to a perfect science talk Be prepared for potential technical...

  20. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... a destination vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick...sequencing and found 98% of the plasmids were perfectly correct. Because they created the assemblies in...
Showing: 181 - 200 of 520 results