We narrowed to 262 results for: INO

1. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   Published

   Jan. 14, 2016, 3:30 p.m.

   ... sequence from a typical reaction, or about 250 amino acids worth, so make sure the feature you want to...

2. Plasmids 101: Golden Gate Cloning

   Type

   Blog Post

   Published

   Aug. 27, 2015, 2:30 p.m.

   ... with an attB recombination scar encoding eight amino acids, but Golden Gate assembly can be designed ...

3. Progress Towards a PAM-Free CRISPR

   Type

   Blog Post

   Published

   March 7, 2024, 2:39 p.m.

   ...knock-in a patient mutation or need to make a precise amino acid change, there is very little flexibility in...

4. Hot Plasmids February 2024

   Type

   Blog Post

   Published

   Feb. 20, 2024, 2:45 p.m.

   ...the epitope tags we know and love, the FLAG tag (amino acid sequence DYKDDDDK) may be one of the most versatile...

5. Hot Plasmids and Viral Preps - March 2021

   Type

   Blog Post

   Published

   March 23, 2021, 1:15 p.m.

   ...fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence) tagged proteins...

6. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   Published

   March 12, 2020, 1:15 p.m.

   ...Szikra, T., Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez, C.P., Keleş, Ö., Kusnyerik, A., Azoulay...

7. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

   Type

   Blog Post

   Published

   May 22, 2018, 1:38 p.m.

   ...Thomas, D., & Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol...

8. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ... which terminus, N or C, to tag and which short amino acid linker to use. These decisions were made based...

9. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   Published

   June 27, 2017, 1:03 p.m.

   ...end positions without changing the order of the amino acids in the protein) may be able to boost FRET ...

10. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ... editing efficiencies of the chimeric and fully albino mice ranging from 11% to 100%, a level similar ...

11. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    Published

    July 11, 2019, 1:26 p.m.

    ...first blog post in this series, I’ll highlight terminology you should be familiar with before working with...

12. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

13. 6 Tips for Grant Writing

    Type

    Blog Post

    Published

    Oct. 18, 2016, 2:30 p.m.

    ...other scientists that we forget that some of the terminology we use routinely isn’t common to non-scientists...

14. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...was not possible, but one of our members took an Arduino microcontroller and a motor controller and made...

15. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    Published

    July 16, 2024, 1:15 p.m.

    ... https://doi.org/10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. ...

16. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ...different trinucleotides (corresponding to different amino acids) in the regions that bind to the antigen. ...

17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...bacteria where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM...

18. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...additional effector domains (4). Taken together, these minor gRNA alterations can be exploited to quickly and...

19. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...viral production. This recombination produces a minor pre-recombined population that does not require ...

20. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...blastx results start the alignment at a more distal amino acid. We consistently run our blastx searches with...