We narrowed to 198 results for: LRE

1. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

   Type

   Blog Post

   Published

   Nov. 2, 2021, 1:15 p.m.

   ... to optimize the serotype, except now you will already have a starting “optimal” injection volume and ...

2. Antibodies 101: Validation

   Type

   Blog Post

   Published

   March 24, 2022, 1:15 p.m.

   ...large-scale proteomic and transcriptomic datasets already exist.   Example: You want to use an antibody to...

3. Typing CRISPR Systems

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   Blog Post

   Published

   March 18, 2025, 1:15 p.m.

   ... candidates for CRISPR proteins. A type VII is already on the horizon (Altae-Tran et al., 2023)! References...

4. Antibodies 101: Designing Your First Flow Panel

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   Blog Post

   Published

   May 28, 2024, 1:45 p.m.

   ...Since CD45 is a common marker, finding an antibody already conjugated to my fluorophore of choice will not...

5. Plasmids 101: Modular Cloning Applications and Kits

   Type

   Blog Post

   Published

   May 14, 2024, 1:15 p.m.

   ...adaptable system with lots of tools and reagents already in place. MoClo is a fantastic way to save time...

6. With an Eye Towards the Future, We Look Back at the March for Science

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   Blog Post

   Published

   Oct. 24, 2017, 1:54 p.m.

   ...Our marches across the world have had an impact already, gaining additional funding and the eyes of the...

7. Custom CRISPR Screens & the Green Listed Software

   Type

   Blog Post

   Published

   July 11, 2017, 2:30 p.m.

   ...which validated inhibitors or even approved drugs already exist. If you are lucky and find that a druggable...

8. Special Delivery: Fluorophore Targeting for FRET Studies

   Type

   Blog Post

   Published

   July 19, 2016, 2:30 p.m.

   ...Addgene plasmids. His-tag labeling reagents You may already have a FRET acceptor-binding site on your protein...

9. A Primer on Optogenetics: Introduction and Opsin Delivery

   Type

   Blog Post

   Published

   Sept. 10, 2015, 2:30 p.m.

   ...opsin you use); the efficacy of optogenetics has already been proven. Therefore, a concrete, well defined...

10. Selecting Your Plasmid Purification Kit

    Type

    Blog Post

    Published

    May 27, 2025, 1:15 p.m.

    ...  The default option is to use the kit that is already available in your lab or the one you are most familiar...

11. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ... guide RNA. But how to test DNA bases that are already snugly paired to each other? At this point, the...

12. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...and Add Another Gene/Insert". The data you have already entered will be saved and the page will open a ...

13. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    Published

    Sept. 10, 2024, 1:15 p.m.

    ...it may be helpful to find an antibody that has already been validated for flow cytometry through this ...

14. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type

    Blog Post

    Published

    Dec. 20, 2017, 2:33 p.m.

    ...enough. Casseus Law suggests that if you are not already reviewing papers that you should start contacting...

15. Troubleshooting and Optimizing a Western Blot

    Type

    Blog Post

    Published

    Sept. 17, 2024, 1:15 p.m.

    ...lower lot-to-lot variability. If you’re running an already-developed assay, and you have faint or no bands...

16. 22 Hot Plasmid Technologies from 2014

    Type

    Blog Post

    Published

    Jan. 6, 2015, 5:21 p.m.

    ...to red when it is oxidized. The Timer tool has already been used by several groups to monitor protein ...

17. Viral Vectors 101: Systemic Capsids

    Type

    Blog Post

    Published

    Oct. 12, 2023, 1:15 p.m.

    ... Cell Reports, 34(13). https://doi.org/10.1016/j.celrep.2021.108754 Ravindra Kumar, S., Miles, T. F., ...

18. 27 Hot Plasmids from 2016

    Type

    Blog Post

    Published

    Dec. 22, 2016, 3:03 p.m.

    ...growing collection of dCas9-VPR plasmids, which already includes dSaCas9-VPR in an AAV transfer vector ...