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Showing: 181 - 200 of 398 results

  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...two-step staining with antibodies. Nanobodies also simplify multicolor staining experiments because they don...epitope-mapping and native protein complex isolation. eLife. PubMed PMID: 26633879 PubMed Central PMCID: PMC4755751...

  3. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...park, part time work, bills, (at times) a social life and rest. I need it just as much in my current role... team. All while still paying bills and having a life outside my job. The same can be said about teamwork...

  4. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...- they relieved some of the pressure of start up life and maintained their sanity by throwing fun into...      Additional Resources Read other posts about life at Addgene!Read more of our careers posts!    ...

  5. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...base pair sequence surrounding your gRNA. Once amplified, you can insert this region into a pCAG-EGXXFP...to the day you have your F0 generation (barring life’s little accidents and stuff ups). This is a huge...

  6. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...knowledge of the software, sharing knowledge allows for life to happen without impacting the overall work. Each...and tidy it up. Conclusion While none of us are life science majors, we do our best to understand the...

  7. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...staining. Direct ELISAs also lack the signal amplification afforded by secondary antibodies and thus are... bind to a single primary antibody and thereby amplify the signal. Since the primary antibody does not...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...photostable than GFP. In addition, antibody-based amplification of their signals is weak and increases background...sites for primary antibodies which can be used to amplify the direct smFP signal within sub-cellular structures...

  10. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...to that of a professional email. Notes from real-life hiring managers In preparation for updating this...that stage, cover letters can be a barrier for qualified applicants. In short, while a cover letter can...

  11. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...two other areas that could potentially improve the life expectancy of mesothelioma patients: early detection...use, they will have a much greater impact on the life expectancy and overall survival of mesothelioma ...

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...possible, especially if the infection is serious or life-threatening, treatment will often begin before this...development of resistance; it’s a natural process of life and one which has allowed bacteria to evolve and...

  13. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...expertise in science careers, women in STEM, work-life negotiation, and mentorship, we decided to have ...questions about science careers, women in STEM, work-life negotiation, and mentorship. We can direct people...

  14. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...University and became a lecturer in the College of Life Sciences that same year. Her research is focused...studying biochemistry at the University of Southern California. He is also an intern at the Yang Lab of the ...

  15. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...acceptor photobleaching method and the fluorescence lifetime imaging microscopy (FLIM) method. Acceptor photobleaching...not to destroy the donor molecule. Fluorescence lifetime imaging microscopy method FLIM has been developed...

  16. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...involving genomic screens, the data stream is so prolific that raw information is made available to everyone...types? And how long should data be archived? Simplifying data access Some of the above mentioned issues...

  18. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...from Gary Yellen’s lab is LiLac, a fluorescence lifetime sensor for lactate developed using a high-throughput... for performance under two-photon fluorescence lifetime imaging. B) LiLac responses are highly resistant...

  19. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...The Gomis-Ruth lab designed the pCri system to simplify the protein purification process by making it ...Illumina-M13F bridging primer. These bridging primers are amplified and used to generate Illumina sequencing libraries...

  20. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ... can virtually eliminate plasma HIV-1, improving life expectancy and quality for HIV-1 patients. However...along with Cas9 in the T-cell line 2D10. PCR amplification and Sanger sequencing of pooled samples showed...
Showing: 181 - 200 of 398 results