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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 463 results for: MEA

Showing: 181 - 200 of 463 results

  1. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...aim for somewhere between 30x and 200x coverage, meaning that you need 30 to 200 times as many colonies ...recombination, we recommend ensuring the cells used are meant for repetitive sequences (such as STBL4 or Endura...

  2. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...suggest retransforming the plasmid into a strain meant for DNA purification. It sounds like extra work,...harvesting and miniprepping at various time points. Measuring the optical density of the culture can indicate...

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...vectors do not have to exclusively be used as a means of detection; they are useful almost anytime you...Glycine and Proline residues found on the C-terminus meaning the upstream cistron will have a few additional...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...Importantly, it is an immune privileged compartment, meaning that it can tolerate the presence of antigens (... size of its DNA cargo - 4.8 kb at most - which means that a gene larger than 3 kb cannot be delivered...

  6. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...going: If your PTA has a general meeting and a means to request funding for events, consider reaching...as coming from networking at the time, but that means it was the best kind of networking! Since nearly...

  7. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ... a stable, easily purified protein with easily measured activity, MDH is particularly amenable to in-class...tag, and compared their specific activities by measuring NADH production.   CUREs are beginning to...

  10. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... natural selection of sequences that evolve a measurable property of a protein. As a proof of concept,... allow for sensitive and high spatiotemporal measurements of norepinephrine. These norepinephrine sensors...

  11. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...complexes, wash away unbound protein, elute and measure the target. However, the two different approaches...the additional benefit of permanent linkage. This means that the primary antibody will not co-elute with...

  12. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ... a result, mesothelioma is often misdiagnosed, meaning that by the time it has caught it has usually started...in the body. Such misdiagnosis and late discovery mean that most mesothelioma patients have an extremely...

  13. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...transient transfection, the vector remains episomal, meaning it stays separate from the genome. One option for...replicate during the course of your experiment, meaning you don’t have to worry about vector replication...

  14. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...frameshift or 21+ bp indel. This score is a useful measure to determine how many of the contributing indels...frameshift or 21+ bp indel. This score is a useful measure for those who are interested in understanding how...

  15. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...plant sciences “transient transformation” usual means that neither a stable cell line or plant is regenerated...GUS and are therefore good temporal reporters to measure promoter activity, for example. Useful positive...

  16. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...think “scientist.” But leaving the bench does not mean you’re not a scientist anymore. Being a scientist...their entire career. Yet even success in academia means you’ll eventually end up as a PI working what is...

  17. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...it’s difficult to get data out of the ELN. This means that reproducibility is limited in two ways – only...external file storage resources remain live. This means that someone who wants to query and attempt to reproduce...

  18. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...you remember everything? Write it all down! And I mean everything! The bare minimum should be taking notes...invaluable. With a little thought you can build meaningful relationships in your new lab, learn the required...

  19. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... with one of these tools, it doesn’t necessarily mean the gene is going to express well. If you do get...PMC3565066. 7. Quax, Tessa EF, et al. "Codon bias as a means to fine-tune gene expression." Molecular cell 59.2...

  20. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...Share science, video usurps the written word as the means for communicating research. Short videos of 3 to... helps researchers go beyond citation rates to measure the impact of their contribution. It offers a 5...
Showing: 181 - 200 of 463 results