We narrowed to 469 results for: NOL
-
TypeBlog PostPublishedFeb. 23, 2017, 1:30 p.m....Writer Meteorologist Journal Editor Textile Technologist/Designer Fieldwork and traveling Many...desired, as roles are becoming more reliant on technology, and good computer skills will give you an advantage...
-
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog PostPublishedAug. 4, 2020, 1:15 p.m....materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently suppresses basal... tagged proteins, a common problem of the AID technology (Yesbolatova et al., 2019). Find the mAID tagging... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog PostPublishedNov. 27, 2018, 2 p.m....Ernesto Valdivia Silva, Universidad de Ingeniería y Tecnología (UTEC), Peru Julio Ernesto Valdivia Silva is ...synthetic biology resources and microfabrication technologies to generate a self renewable sensor, a bacterium... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....developers’ willingness to make their CRISPR technologies available to the academic research community...Addgene, we’ve helped distribute many of these technologies in plasmid form and are proud to have facilitated... -
Resume Writing for Non-academic Science Careers
TypeBlog PostPublishedDec. 18, 2014, 4:06 p.m....presented chronologically. With a resume however, most often people choose a reversed chronological style ... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....allow a protein to be more effectively used in a technology or as a therapeutic. A (very) brief history ... mostly accessible to experts. That many GCE technologies have idiosyncrasies presents a significant barrier... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses. 2011 Jun;3(6):677-713...An Overview of the Application of Viruses to Biotechnology. Viruses. 2021 Oct 14;13(10):2073. DOI: 10.3390... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. In my previous...guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has had nearly ... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog PostPublishedMay 9, 2017, 2:12 p.m.... is one of the latest developments in CRISPR technologies. It’s a simple and effective method with multiple...CRISPR–Cpf1 Asing a Single crRNA Array." Nature Biotechnology 35.1 (2016): 31-34. Pubmed PMID: 27918548. 2... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog PostPublishedDec. 12, 2019, 2:22 p.m....visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items. The technology behind Addgene’s new search Like millions of... -
Hot Plasmids - May 2022
TypeBlog PostPublishedMay 26, 2022, 1:15 p.m.... for example). Given that the liver is an immunologically active organ, this advance is likely to afford...Kaufman, et al, 2022. This new barcoding technology would allow researchers to avoid the off-target... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....multiprotein complexes. Fig. 2. Multiplex immunolabeling with subclass-switched recombinant antibodies...monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:e43322. doi:... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....live-cell imaging, single-molecule tracking, immunolabeling of fixed cells, Western blotting, and more....future genetic screens. DeWeirdt PC, et al. Nat Biotechnol. 2021. doi: 10.1038/s41587-020-0600-6. The Penn... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....you have any questions or thoughts about this technology, leave them in the comments below. References... Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Jun;32(6):569-76. doi: 10.1038/nbt.2908... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's... require access to next generation sequencing technology. These libraries are lentiviral in nature, ... -
To Each HIS Own
TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m....Proteins with a Novel Metal Chelate Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff...growth factor I from culture medium of E.coli. Bio/Technology. 1987, 5:379-382. Bornhorst JA and Falke JJ. ... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....References and resources References Bendall SC, Nolan GP, Roederer M, Chattopadhyay PK (2012) A deep profiler... profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it.2012.02.010 Dennler... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016): 528-30. PubMed PMID: 27088723.... -
Transferable Skills Guide: Cross-team Communication
TypeBlog PostPublishedNov. 21, 2019, 1:53 p.m.... the lab to be using words for their field or technology in a consistent way. Just creating a lexicon ...first weeks or months when I was learning the terminology of a new field. Annotate: When creating important...