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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: Ncl

Showing: 181 - 200 of 628 results

  1. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...you realize whether or not you have a natural inclination for this skill that you could strengthen. The...everyone who visits your poster to agree with your conclusions. That conversation you’re having--that conflict...of the conflict. It can be easy to leap to the conclusion that a conflict is personal (”Person A and Person...

  2. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...chloramphenicol-resistant strains, we have also included versions with gentamicin-resistance (amplified...broad group of Gram-negative Enterobacteriaceae including in E. coli O157:H7, Shigella flexneri, Enterobacter...where conjugation may be difficult, we have also included versions of pTOX that encode one of two visible...

  3. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...qualitative and quantitative measurement of processes including RNA movement by tagging RNA sequences with an ...Kellenberger et al., 2013). Other variants of Spinach include Baby Spinach, a miniaturized version of Spinach...aptamer fluorophores, more are in development, including Carrot and Radish from the lab that developed ...

  4. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...have been used to purify a variety of peptides including antibiotic efflux pumps and ankyrin seven-repeat...constructs. Some applications of plasmids from this kit include the production of a suicide switch in cyanobacteria...PUF protein fusions reported in the literature include RNA detection, RNA cleavage, and translational ...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...choose a looser gate here. I am not worried about including irrelevant events in my analysis, as these will...Lastly, you learned which gates should always be included in your analysis, regardless of your experiment...Sharpe at Harvard Medical School. His projects include investigating negative regulators of T cells and...

  6. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...integrins are key to a diverse array of processes, including cell adhesion, migration, and signaling, as well...available through Addgene’s repository. The collection includes 26 unique antibodies that bind discrete subunits...domains. All bind specific integrin alpha subunits, including αL, αM and α5. IPI’s anti-integrin rAbs are unconjugated...

  7. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...N-terminus, but you don’t have the tag already included in the vector you want to use. If you inserted...primers to amplify the two fragments while also including regions of homology to the vector or neighboring...sequences between fragments. Some of these products include: GeneArt® Seamless Cloning and Assembly (Thermo...

  8. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...resources needed to accomplish your science, and can include: equipment, reagents, animals, glassware, and even...the institution/funding source. Major equipment includes large centrifuges, sequencers and analyzers, and... as many of these tools as possible, this even includes clocks, timers, and phones.  As for reagents and...

  9. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...works. Advances in Cre-lox recombination have included inducible Cre, such as the tamoxifen-responsive...immediately following the promoter. The STOP cassette includes a mutant YFP that does not fluoresce, but can ...More variants have been created by other labs, including R26R-Confetti described in Hugo J. Snippert, et...

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...phenotype in order to conclude that the phenotype is due to an on-target effect. Conclusions Selection of gRNAs...modification of interest, it would be unwise to make conclusions on the basis of the activity of a single gRNA...

  11. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...expression, but causality and mechanisms remain unclear. Today, targeted epigenetic modification at specific...which uses light to regulate transcription, also includes light-regulated histone methyltransferases and...has deposited mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300...

  12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    .... Other practices that fall into this category include performing experiments with too few replicates ...institute better experimental design practices including talking to statisticians when necessary and encouraging...Hines Minisymposium on Reproducibility Booklet - Including descriptions of many organizations promoting reproducibility...

  13. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ... cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive chimeric GPCRS (OptoXRs) (Tichy...experiments!  First, closely read the publication, including the supplemental data. You’re most likely to find...hardware, optogenetics tools, viruses, and more.  Conclusion   Optogenetic tools provide a powerful way to...

  14. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...mentioned above. The initial IMP cohort of 78 mentees included: 47 women (60.2%) 20 graduate students (25.6%...directly attributable to participation in the program included improvements in writing fellowships, giving oral...impressions of the IMP experience. Conference highlights included remarks by the Spanish Secretary of Research, ...

  15. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...reproducibility of experiments and the validity of conclusions. Antibody engineering also benefits antibody-...antibody. (B) Examples of engineered antibodies, including chimerization, conjugation, and antibody fragments...There are different ways to achieve this goal, including error-prone PCR, degenerate primers, gene shuffling...

  16. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...Wild-type A549 cells do not express CAs9 and are included as a control (WT). Vinculin is a loading control... assay before, consider following their tips, including: Before digesting the DNA duplexes with Surveyor...PAGE instead of agarose to get sharper bands. Conclusion Overall, making a human cell line with a gene...

  18. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...Science is about fighting scientific partisanship, including more scientists in our leadership, and raising... Anschutz Medical Campus.  He studies viruses including HIV and is interested in global health policy....Committee about climate change. All the scientists – including global warming skeptics – insisted that more data...

  19. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e. HTRF and LanthaScreen...dynamic range of your assay. These experiments include producing a positive control construct where the... Women’s Hospital in Boston, MA. His interests include developing novel fluorescent tools for labeling...

  20. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ... in Table 2 is a standard and should always be included—the live/dead stain. This stain is in fact not...www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer.   Conclusion You made it! In this post, you learned about ...Sharpe at Harvard Medical School. His projects include investigating negative regulators of T cells and...
Showing: 181 - 200 of 628 results