We narrowed to 230 results for: Ott

1. A Career in Grants: How To Become a Grant Professional

   Type

   Blog Post

   Published

   Aug. 20, 2015, 2:30 p.m.

   ...competencies through a multiple choice exam.  The bottom line on being a grants professional The Grants ...

2. Negotiating Work and Life: How to Find the Joy

   Type

   Blog Post

   Published

   March 20, 2014, 3:04 p.m.

   ...time with your friends – no spouses or kids. The bottom line We don’t just “do science” – we are scientists...

3. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

   Type

   Blog Post

   Published

   July 8, 2025, 1:15 p.m.

   ...top). Overhangs must match the Level 0 acceptor (bottom) for CDS1 parts. (B) Example Level 1 assembly. ...

4. Hot Plasmids - November 2023

   Type

   Blog Post

   Published

   Nov. 14, 2023, 3:35 a.m.

   ...fact, Anti-PSD-95 [K28/43R] has been one of the hottest antibodies in Addgene’s collection since we began...

5. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

   Type

   Blog Post

   Published

   Jan. 24, 2023, 2:15 p.m.

   ...-MB-231 cell lysate after 4-days of expression (bottom, scalebar = 0.5µm). (d) Ultrasound imaging of MDA-MB...

6. New CRISPR Tools: Cas7-11 and PASTE

   Type

   Blog Post

   Published

   Feb. 17, 2022, 2 p.m.

   ...whether the samples are from a rain gutter or the bottom of Tokyo Bay. When a weird, sulfurous smelling,...

7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...doi.org/10.1038/nature26155 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O,...

8. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   Published

   April 16, 2020, 1 p.m.

   ...access to reagents and equipment has become a bottleneck. In the last few years, scientists have utilized...

9. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

   Type

   Blog Post

   Published

   July 23, 2019, 12:46 p.m.

   ...the LY6A receptor to gain access to the CNS. The bottom line of viral vector permissiveness While PHP.B...

10. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    Published

    July 2, 2019, 12:51 p.m.

    ...top) and neuron-specific expression of dCas9-VPR (bottom). Created with BioRender.com. The Day Lab used...

11. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ... Disease & Open Science: Interview with Darrell Kotton Four Factors that Differentiate the Stem Cell Field...

12. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ... by producing a detectable signal in urine. The bottom line with plasmid addiction systems While selfish...

13. How to Write a Scientific Review Article

    Type

    Blog Post

    Published

    Feb. 16, 2017, 3:30 p.m.

    ...overthink it. And to quote my adviser, "you just gotta do it." At the end of the day, someone has to write...

14. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    Published

    Aug. 18, 2016, 2:30 p.m.

    ...Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J,...

15. The Challenges of Cell Culture

    Type

    Blog Post

    Published

    May 5, 2016, 2:30 p.m.

    ...public databases. Image attributions (from top to bottom) Database Center for Life Science (DBCLS),  National...

16. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type

    Blog Post

    Published

    Oct. 27, 2015, 3 p.m.

    ...did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health...

17. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    Published

    Aug. 5, 2014, 6:51 p.m.

    ... August 2013) PLOS’ Bold Data Policy - by David Crotty (The Scholarly Kitchen) Read more Addgene blog...

18. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ...Once you've gotten to know what a plasmid is in general, it's time to take a look at some of its parts...

19. Hot Biosensors 2022: Year-End Roundup

    Type

    Blog Post

    Published

    Dec. 6, 2022, 2:15 p.m.

    ...targets to measure! Let’s take a look at some of the hottest fluorescent biosensors new to Addgene this year...

20. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    Published

    May 20, 2021, 1:15 p.m.

    ...wireless monitor. Wireless video technology has gotten very impressive, but due to costs and availability...