We narrowed to 552 results for: PPL;
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TypeBlog PostPublishedJune 13, 2017, 2:30 p.m.... be mostly on the brain, but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts... infect) can influence an AAV’s value greatly. Applying this mantra to AAV selection, there are three ...only can we compare these methods in specific applications, we may also be able to use these comparisons...
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RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....silencing can be easily induced by exogenous application of long or short dsRNA. However, it is important...have benefited from the ease of use in dsRNA applications. Figure 1: Schematic of an RNAi-inducing..., which is conserved in many organisms. When supplemented with adenine analogs such as 2-fluoroadenine... -
9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m.... have been completed. Therefore, to be ready to apply for a Group Leader position, key first author manuscripts... project. Furthermore, Group Leader position applications require 3 letters of reference- the graduate...informal recommendations, more positive grant application and manuscript reviews, etc. You'll also meet... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....Shakespeare for the first time. The discovery and application of CRISPR-Cas9 kicked off over a decade of frenzied...mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Molecular Cell, 84(3), 463-475.e5. https://...Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system. Gene and Genome... -
New Antibodies in the Lab Animation!
TypeBlog PostPublishedNov. 19, 2024, 2:15 p.m....Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications. Thanks for watching, and we hope you found... -
Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab
TypeBlog PostPublishedSept. 7, 2021, 1:15 p.m....New to the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the...techniques. It’s also great for teachers looking to supplement their curriculum. From the details about personal... -
We’re Going Viral This Week!
TypeBlog PostPublishedOct. 14, 2019, 1 p.m.... information on the basics of viral vectors, applications, and some new resources available from Addgene... first paper came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to ... -
#Deckthelab Contest Winners 2017
TypeBlog PostPublishedJan. 31, 2018, 2:44 p.m.... a lab supply tree and set a challenge for her lab mates to create the best-recycled lab supply ornament... -
Antibody Data Repositories and Search Engines
TypeBlog PostPublishedOct. 31, 2024, 1:15 p.m.... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any... -
CRISPR Cheat Sheet
TypeBlog PostPublishedJan. 27, 2021, 2 p.m....an organism’s genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences ...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the... -
Summer Fun at Addgene!
TypeBlog PostPublishedMay 12, 2022, 1:15 p.m.... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of... -
Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana
TypeBlog PostPublishedDec. 20, 2016, 3:30 p.m....awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...scientist's molecular biology needs and why Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:... -
Editor's Choice, July 2016
TypeBlog PostPublishedAug. 5, 2016, 3 p.m....target and use FRET flurophores for a variety of applications. While editing this post, I learned a lot about...this post to anyone wondering if and how they can apply FRET in their own research. All posts from July ... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog PostPublishedNov. 12, 2014, 3:54 p.m....that it is also possible that their system may be applied to efficient viral vector-mediated multiplex genome...genes at the same time.” For more details on how to apply the new system, see Yamamoto and Sakuma’s protocol... -
Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m....significant advances in the understanding and application of CRISPR/Cas9 technology are coming along at...of Chase L. Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing an antibiotic... -
Microbes: Look, Listen, and Tell
TypeBlog PostPublishedAug. 28, 2018, 12:04 p.m....cellular imaging with ultrasound. In his research he applies naturally occurring bacterial “molecular buoyancy...biomolecule-based dyes. This technology is widely applicable as bacterial expression of dyes allows dye visualization... -
Designer PUF Proteins for Any RNA Target
TypeBlog PostPublishedJune 17, 2014, 7:57 p.m.... The new tool was developed and implemented by applying the Golden Gate cloning method to human proteins...says the new tool might even find therapeutic application in addition to their use in basic biology, for... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog PostPublishedMay 17, 2016, 2:30 p.m....with the gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications we currently... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m....Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion One concern... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF...GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight is suitable for...