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We narrowed to 599 results for: REV;

Showing: 181 - 200 of 599 results

  1. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...approach, your workflow, or your physical setup - to prevent the mistake, just like you would change variables...reagents from left to right as they use them.    Prevent Interruptions If you need to focus, a large set...Down At the suggestion of a postdoc, to whom I am forever grateful, I started setting aside 3-5 minutes to...

  2. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...internship. Lastly, have your resume and cover letter revised by your network, which includes your friends, family...personality leap out of the page. Finally, as previously mentioned, enlist the help of your network to...week before the deadline. The day of submission, review your application with a fine comb and hit submit...

  4. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...altered by blue light illumination. This ability to reversibly bind and release a protein of interest in response...allows for tagless protein purification as well as reversibly regulation of endogenous signaling activity in...while cycling between dark and blue light which revealed a blue light-dependent shift in mCherry fluorescent...

  6. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...formaldehyde or UV light to create strong but reversible bonds. This will keep the protein:DNA complexes...washing and then incubate with Proteinase K to reverse the crosslinking. Finally, purify your DNA with...Shengliu via Wikicommons.   Okay, let’s do a quick review. At this point you have (1) crosslinked your proteins...

  7. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...methyltransferases, methylate DNA at particular sequences to prevent them from being degraded by restriction endonucleases...used for long term storage. Additional Resources Review of restriction modification systems NEB's Resource...describing Dam and Dcm Methylases Discovery of DpnI Review describing dam-/dcm- E. coli Resources on the ...

  8. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...next-generation sequencing. Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing...16S rRNA isolated from the microbial community) reveals the RBS and allows translation of the reporter ...Cameron, et al. "A distributed cell division counter reveals growth dynamics in the gut microbiota." Nature ...

  9. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ... are two heart defects that are 50 times more prevalent in women with TS. “The goal of our study is to...chromosome gene dosage in TS cells as a first step to reverse the developmental defects that cause heart problems...monitor intracellular signaling,” says Hébert. Previous research has shown that GNB1 mutations lead to...

  10. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...Advanced microscopy, University of Amsterdam. The previous post in this series described a practical approach...photostable fluorescent protein. Photobleaching is the irreversible destruction of a fluorophore under the influence...photobleaching, fluorescent proteins may display reversible intensity changes (Shaner et al, 2008; Bindels...

  11. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...marriage of two simple platforms CRISPR has revolutionized how we decode the genome by making it easy ...CACCGNNNNNNNNNNNNNNNNNNNGTTTGGGTCTTCGAGAAGACCTATTCXXXXXXXXC -3’; Reverse oligo: 5’- AATTGXXXXXXXXGAATAGGTCTTCTCGAAGACCCAAACNNNNNNNNNNNNNNNNNNNC... Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities...

  12. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146/annurev-biochem-051410...transcription start site function would also help to reveal the impacts of a lncRNA on nearby genes and its...

  13. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...field forward to where it is today. Below we will review some of the CRISPR-based editing therapies that...’s own cells that have been modified ex vivo, previously edited healthy donor cells can be delivered immediately...safety is important for allogenic therapies to prevent a graft vs. host response. Off-target effects are...

  14. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...therefore already have ways to prevent AAV infection. For example, prevalence of neutralizing antibodies ...or potentially curing human diseases that were previously thought to be incurable. Rapid advances in next...

  16. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...Editing Technology at Beam Therapeutics, used a previously developed bacterial selection strategy (Gaudelli...more efficient. Informed by these mutations and previously described TadA mutations (Gaudelli et al., 2017...mitigated with the expression of fetal hemoglobin. Previous studies have shown that one base change in the...

  17. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...mutations in the PMM2 gene. Videira’s group recently reviewed and reported that PMM2-CDG patients suffer from... congenital disorders of glycosylation (CDG): a review. J. Inherit. Metab. Dis. 39, 765–780 (2016). PubMed...acetylcholine. Given its unique molecular biology and previous approval by the FDA, GdnHCl could, in principle...

  18. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...symposia held across the USA during this time have revealed that young scientists are aware of, and are engaged...Academy of Sciences. “The Postdoctoral Experience Revisited” 2014 Appendix B p93-95). For those advocating...25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations from the...

  19. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...a table. It adds a formality and distance that prevents interaction with each other and the audience. ...moderator takes control It is the moderator's job to prevent anyone from derailing the show. The moderator must... active at all times keep things moving and to prevent any one panelist or audience member from taking...

  20. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited...genetically-encoded voltage indicator (GEVI) than previous versions. ASAP5 can be imaged using one- or two-photon...across many cell types in varying conditions, revealing context-dependent responses and molecular patterns...
Showing: 181 - 200 of 599 results