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We narrowed to 488 results for: Ret;

Showing: 181 - 200 of 488 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain...

  2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...particularly effective as it can result in the retention of introns or the removal of exons. Disrupting...microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014;11(10):1243-1249. doi:10.1080...

  3. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...resistance genes to help cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids...Resources References Demanèche, S., Bertolla, F., Buret, F., Nalin, R., Sailland, A., Auriol, P., Vogel,...

  5. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...access it later. Pro tip Checking the number of retweets, likes, and comments on a tweet about a paper ...papers are connected via citations, in order to return results that are more relevant to your search terms...

  6. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...than pre-designed sets, so identical searches can return multiple answers. You might want to take advantage...fragments, or defined split regions. SplitSet will then return the highest-fidelity overhang set it can find for...

  7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...quantification of various intermediate steps, a sizing electrophoretic gel, and qPCR quantification of the final libraries...assembly. Most of the time, these plasmids are returned as one or two partial assemblies. If we obtain...

  8. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ..., and Martin L. Yarmush. "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent...., et al. "Expansion and fibronectin-enhanced retroviral transduction of primary human T lymphocytes for...

  9. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...depending on the cell line so old cells should be retired! Conversely, cells younger than the 3rd – 4th passage...

  10. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...scientists have taken advantage of the natural secretion of biochemicals and proteins through plant roots... Tagging recombinant proteins with a naturally secreted plant peptide allows for easier access and purification...

  11. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...fluorescence falls within the linear window for the theoretical timer shown in Figure 1B, you can use the equation...differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells." Nature communications 7 (2016...

  12. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...activators or repressors. These fusion proteins retain the ability to recognize and bind to DNA; when ...occasions truncated gRNAs (≤15nt) have been found to retain some ability to induce Cas9 to make edits (AC, ...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...control of biological systems (Wheeler et al., 2016; Duret et al., 2019). But the field has sometimes seen ...plasmid here!   References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas...

  14. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually introduced...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...

  15. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...standard curve, and most results can be confidently interpreted as integers. Since each sample is partitioned...method you choose, resist the temptation to over interpret your results: Sybr cannot be used to determine...

  16. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...much easier. So you could imagine engineering the entirety of a cell. That's a big long-term technical challenge... What's incredible is the bioeconomy is already pretty substantial. It's estimated that genetic engineering...

  17. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ... bathroom mirror pep talk, forgotten and then retrieved your lucky socks, persevered through a nerve wracking... snapshot of the skills that you can bring into pretty much any other workplace. To get your mind working...

  18. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification...production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized and ...

  19. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...within the target window, possibly due to longer stretches of duplexed RNA. Using an ADAR2DD catalytic mutant...perform C-to-U edits, the authors note that it retains its adenine deaminase ability. The authors then...

  20. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...appear reduced in ExM, which could impact how you interpret a target’s structure. For example, cytoskeletal..., Chen F, Piatkevich KD, et al (2016) Protein-retention expansion microscopy of cells and tissues labeled...
Showing: 181 - 200 of 488 results