We narrowed to 297 results for: SAM activators

...gene, as well as to study the effects of inactivating/activating mutations. In biomedical research, modeling...insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly and GeneArt cloning...plasmid. For insertions, it’s possible to use the same technique just using a large primer known as a megaprimer...

...human pluripotent stem cells: a transcription activator-like effector nuclease (TALEN) kit consisting ...of individuals and find several mutations in the same gene or in multiple genes, all of which seem to ...insert all of those variants into stem cells with the same genetic background and do an apples-to-apples comparison...

... expertise, publications, and plan for future activities; including the interest of the greater scientific...least 3 full-time people engaged in researcher activities. The EB-1B requires that you provide two out ...working in October of the same year. The Industry H-1B visa is not the same as an academic H-1B visa and...journal editors to request to be a reviewer. Proactivity is very important in this case.  You can still...individually, as a judge of the work of others in the same or allied academic field Evidence of original scientific... prestige of the employer makes EB-1Bs a very attractive option.    Your Expert Letters of support can...

...Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation." Nature biotechnology...has been surrounded by a whirlwind of research activity and development since its inaugural year in 2012...that the CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over...later it became quite clear that others thought the same. Since January 2013 there have been 33 CRISPR software... “HTML”, “visual track”, “plot”, “tabular”, “interactive”, and “save”. Trends: Most tools provide tabular...knowledge from laboratories employing CRISPR on the same organism could be used as a proxy for this last ...

...and more productive. Higher Productivity. Taking active measures in improving cross team communication ... and engineers were attending the same meetings and hearing the same words, they were leaving these meetings...aforementioned lab manager, said the word “sample,” did the word convey the same concept to Morgan, our senior engineer...help get everyone on the same page. Others in the meeting might also have the same question and asking can...When everyone at a meeting is using the same words with the same meanings consistently, meetings will be...counsel, used the word “service,” did he mean it the same way as Bryan, our customer support manager? When... an Addgene-specific definition. Words such as “sample,” “material,” “item,” and “format” all received...

...antibodies, although not all isotypes have the same ability to activate each of these functions: Recruitment ...appropriate isotype control. An isotype control has the same constant heavy chain domain as your primary antibody...results in the B cell expressing an antibody with the same variable domain but a different heavy-chain constant...

...with a little mutagenic assistance). Quicker CAST activity allowed certain mutated phages to outcompete their...they met the directed evolution expertise of the Samuel Sternberg and David Liu labs. Evolving CASTs for...

...scientific publishing process. There is a rich and active community of science communication professionals...aspects of bioscience into context, while at the same time working through the details of experimental...

...also observed reliable optically evoked neuron activity for at least several months after implantation...interact during various behaviors. Either tissue samples were harvested after the behavior, or the animals...

...from “TGA” to “TGG.”  After identification of ABE activity, XMAS-TREE can also be used to purify these modifications...found in the metagenome datasets from hypersaline samples, were engineered and adapted for RNAi experiments...

... quantitative assays such as antibody specific activity for each reagent batch. To supplement this, Addgene...blog post. Hello, NABOR! Addgene will bring the same open-access benefits to antibodies that you have...

...untagged control could enter cultured cells and activate expression of a luciferase reporter. CPP was not... better than immortalized lines derived from the same cell type; however, their usage has been limited...

...dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable single guide RNA (...The multiple scRNAs all individually utilize the same dCas9 protein. By using an inducible promoter to...

...product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced...produce the same signal, so only one HRP-conjugated RANbody can be used at a time. Samples can be sequentially...fluorescently labeled tyramide molecule into a highly reactive radical, which is then covalently linked to residues... lower background and better penetration of the sample due to the smaller size of RANbodies compared to...sequentially stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible...it is approximately as sensitive as detecting the same antigen with a standard primary/labeled secondary...staining of tissue because they have limited cross-reactivity with commonly used anti-rodent or -rabbit secondary...

...HRP, a chemiluminescent conjugate that can be activated to emit light with a simple kit, and is readily... the antibodies time-sensitive, using precious samples, or being compared to other data in a manner that..., simple to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason...signaling molecule, that all need to be detected in the same assay. This is one of the most complex subjects ...

...Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc 12:828–863 . https://doi.org...holes are then filled with other samples in our queue such as viral samples for Viral Genome Sequencing (...preps and pooled libraries.  Viral samples are filled into the same process as our normal Complete Plasmid...unique barcodes per sample so we can assign sequencing reads to the correct sample. The PCR is then performed... process that yields high-quality isolated DNA samples of sufficient quantity for sequencing. This process...yielding anywhere from two to six plates of 96 samples per week. This is where our new process will begin...of a pipeline that takes the raw data from each sample in our pool and individually assembles the reads...

...variety of ways. How do scientists focus on the activity or function of a single protein? Oftentimes, they...metal ions are immobilized on a bead or resin and a sample containing the protein of interest flowed through...interest and the metal ions.      Fig. 1: A sample containing a polyhistidine tagged protein of interest...tags are also recommended if you need a very pure sample. Because binding strength is proportional to the...

...variety of applications: base editing, CRISPR activation and inhibition, among others. Over the past few...combination of MS2, PP7, or boxB sequences in the same sgRNA will generate additional colors. Image from...

...time, we have also demonstrated our technology on active virus, as well as shown that we can detect the ...high and above what would be expected in patient samples (e.g.: blood, urine). Adapting toehold switches...pathogen’s genome as unique barcodes to identify samples containing the virus. Of the 48 sensors, targeting... diagnostic results.  Our approach now detects samples at clinically relevant concentrations (1-3 fM; ... look at viral load in patient blood and urine samples; we showed that we can detect the virus directly...Interview with Previous Collin's Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes...

...brain, mapping neuronal networks, and selectively activating signaling pathways. Many scientists came to the...While walking around the exhibition area, I got a sampling of the broad variety of tools in neuroscience ...