We narrowed to 251 results for: SPI;

1. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   Published

   Nov. 5, 2024, 8:32 p.m.

   ... of your baculovirus is straightforward — simply spin down the supernatant in a centrifuge to remove any...

2. Magnetic Control of Proteins: More than a Dream

   Type

   Blog Post

   Published

   Sept. 24, 2024, 1:15 p.m.

   ...deposited their plasmid with Addgene, hoping to inspire others to explore the phenomenon, improve the tool...

3. Pooled Library Amplifications

   Type

   Blog Post

   Published

   May 30, 2024, 1:30 p.m.

   ... Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 144-188 bp 100 ...

4. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   Published

   Dec. 1, 2016, 3:30 p.m.

   ... inactivate specific genes in mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in...

5. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ...postdoctoral training from Massachusetts General Hospital and Harvard Medical School. Connect with her on...

6. Plasmids 101: Optimizing Plasmid Yields

   Type

   Blog Post

   Published

   May 26, 2016, 2:30 p.m.

   ..., or a home-brew optimized for plasmid yields. Spiking your media with supplements such as magnesium salts...

7. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...Vanderperre S, Paul R, Björklund M, Ahr D, Ahmed AW, Spinelli L, Brun C, Basler K, Merabet S (2018) Generation...

8. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   Published

   July 16, 2020, 1:15 p.m.

   ... the U.S. (42%), Europe (23%), and China (14%). Spikes in plasmid requests correspond to publications ...

9. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ...begin and end on time? Did the conversation remain spirited and focused as opposed to a wandering monologue...

10. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    Published

    Sept. 28, 2017, 2:01 p.m.

    ...gene-transfer tools in the study of the brain and spinal cord, which together are known as the central nervous...

11. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type

    Blog Post

    Published

    July 1, 2025, 1:15 p.m.

    ...flow-through solutions and use clean tubes? How about spin times, were these long enough?  There are, of course...

12. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    Published

    Aug. 13, 2024, 1:15 p.m.

    ...Brechmann et al. al., 2021) while the media is aspirated. The beads are then washed, followed by antibody...

13. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ...sense of what AdVs are about – and we hope that it inspires further use of existing vectors, as well as research...

14. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    Published

    Feb. 9, 2023, 2:15 p.m.

    ...Kimball et al., 2020). Benefits and Challenges CUREs inspire students to take ownership of their work and become...

15. Uncovering Ancestral Bias in CRISPR Research

    Type

    Blog Post

    Published

    Nov. 29, 2022, 2:15 p.m.

    ...'s Genetic Perturbation Platform to modify the CRISpick algorithm to prevent guides from being designed...

16. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    Published

    Dec. 3, 2019, 11:38 a.m.

    ...Flame is more than an award for depositors and inspiration for our updated mascot, it’s also a convenient...

17. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    Published

    March 17, 2016, 2:30 p.m.

    ...Karl-Klaus Conzelmann. "Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is...

18. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...that extra two miles which is way more fun. And despite those who may wish to tell you so – towel corners...

19. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ... slightly higher, than those of agarose beads, despite the size difference. Magnetic beads are particularly...

20. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements, and ...