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We narrowed to 497 results for: SUB

Showing: 181 - 200 of 497 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... be helping the untagged protein enter cells. Subsequent work found that both NaCl and non-detergent sulfobetaine... be used to transduce Cas9 and a gRNA, which subsequently enter the nucleus and cleave the specified target...

  2. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...conditions will derail a research program (think a subtle difference in mouse chow, a different water filtration...did. Admittedly, the differences are often very subtle, and it can take a lot of detective work to figure...

  3. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...hairpins into the gRNA tetraloop and went through subsequent design iterations to increase stability and minimize... used to visualize relative locations of the subtelomeric region of the pericentromeric region (PR1), ...

  4. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ... could not enter cells expressing murine ACE2 (Subbarao et al., 2006). Now that a similar phenomena is...: . https://doi.org/10.1038/s41467-020-15562-9 Subbarao K, Roberts A (2006) Is there an ideal animal model...

  5. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...Every few months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository... here!   Andrews NP,  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

  6. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ... as a whole or can be used to isolate specific subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can... lymphatic system or even the human skin and subcutaneous models (Groell et.al, 2018). Treating these ...

  7. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...bodies are a polymer of many copies of monomeric subunits. Unlike cytoskeletal polymers, however, R bodies...propagating through an enormous lattice of identical subunits. A brief history of R bodies R bodies have been...

  9. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...freeze-thaw-freeze cycle before, or otherwise been subject to mishandling?  Is the experiment you’re planning...the same assay. This is one of the most complex subjects in antibody-based protocols - but thankfully this...

  12. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...promoters commonly used in plant research. Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit ... releases heat shock factor 1 (HSF-1), which subsequently binds to heat shock elements in the promoter...

  14. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...It should be no surprise then that a lot of our subject matter comes straight from you, whether you realize...second review from someone less familiar with the subject for clarity. Our content covers a lot of very ...

  15. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...CLOVER Center at CalTech? If so, you may have submitted a question that didn’t get answered live - but...Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors Blog Posts ...

  16. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...MFI makes it easier to identify and study these subtle shifts. Additionally, you can gate off the histograms...the presence/absence of your marker but there are subtle shifts in expression, using a histogram plot and...

  17. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...transgenes via increased nuclear export. LTR Subcomponents: -U3 -R -U5 LTR; Long terminal repeats; U3...bigger than ∼3 kb are packaged less efficiently. Subcomponents: U3; Unique 3'; region at the 3' end of viral...

  18. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside... genes that allow for the use of a particular substrate in defined medium, without which the cells cannot...

  19. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-ligase IV); together with some...Aprataxin, Tdp2) that function to make ends better substrates for ligation. Although we do not describe these...

  20. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. coli...experiments. These background proteins were then subtracted from Ramanathan et al.’s RaPID datasets. Applications...
Showing: 181 - 200 of 497 results